Peter D. Burbelo
A5041450311- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- Salivary Gland Disorders and Functions
- HIV Research and Treatment
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Herpesvirus Infections and Treatments
- Protein Kinase Regulation and GTPase Signaling
- T-cell and B-cell Immunology
- Cellular Mechanics and Interactions
- Hepatitis C virus research
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Hepatitis B Virus Studies
- Biosensors and Analytical Detection
- Adrenal Hormones and Disorders
- SARS-CoV-2 detection and testing
- Viral-associated cancers and disorders
- Parvovirus B19 Infection Studies
- Kawasaki Disease and Coronary Complications
- Animal Virus Infections Studies
National Institutes of Health
2016-2025
National Institute of Dental and Craniofacial Research
2016-2025
University of Maryland, Baltimore
2021
National Institute of Diabetes and Digestive and Kidney Diseases
2012-2021
Government of the United States of America
2021
Nationwide Children's Hospital
2018
North Carolina State University
2018
Columbia University
2018
University of Copenhagen
2018
Hvidovre Hospital
2018
Interindividual clinical variability in the course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is vast. We report that at least 101 987 patients with life-threatening disease 2019 (COVID-19) pneumonia had neutralizing immunoglobulin G (IgG) autoantibodies (auto-Abs) against interferon-ω (IFN-ω) (13 patients), 13 types IFN-α (36), or both (52) onset critical disease; a few also auto-Abs other three type I IFNs. The neutralize ability corresponding IFNs to block...
Integrin receptors play important roles in organizing the actin-containing cytoskeleton and signal transduction from extracellular matrix. The initial steps integrin function can be analyzed experimentally using beads coated with ligands or anti-integrin antibodies to trigger rapid focal transmembrane responses. A hierarchy of actions was identified this study. Simple aggregation triggered localized accumulation 20 molecules, including RhoA, Rac1, Ras, Raf, MEK, ERK, JNK. In contrast, out...
Despite signs of infection—including taste loss, dry mouth and mucosal lesions such as ulcerations, enanthema macules—the involvement the oral cavity in coronavirus disease 2019 (COVID-19) is poorly understood. To address this, we generated analyzed two single-cell RNA sequencing datasets human minor salivary glands gingiva (9 samples, 13,824 cells), identifying 50 cell clusters. Using integrated normalization annotation, classified 34 unique subpopulations between gingiva. Severe acute...
Rho, Rac, and Cdc42 are small GTPases that regulate the formation of a variety actin structures assembly associated integrin complexes, but little is known about target proteins mediate their effects. Here we have used motif-based search method to identify putative effector for Rac Cdc42. A GenBank™ data base similarity with minimum Cdc42/Rac interactive binding (CRIB) region potential protein p65PAK has identified over 25 containing similar motif from range different species. These...
Rac and Cdc42 regulate a variety of responses in mammalian cells including formation lamellipodia filopodia, activation the JNK MAP kinase cascade, induction G1 cell cycle progression. is also one downstream targets required for Ras- induced malignant transformation. containing Y40C effector site substitution no longer intact with Ser/Thr p65PAK are unable to activate pathway. However, they still induce cytoskeletal changes an F37A substitution, on other hand, interacts p160ROCK or We...
Autoantibodies against interferon-γ are associated with severe disseminated opportunistic infection, but their importance and prevalence unknown.We enrolled 203 persons from sites in Thailand Taiwan five groups: 52 patients disseminated, rapidly or slowly growing, nontuberculous mycobacterial infection (group 1); 45 another without 2); 9 tuberculosis 3); 49 pulmonary 4); 48 healthy controls 5). Clinical histories were recorded, blood specimens obtained.Patients groups 1 2 had CD4+...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of disease 2019 (COVID-19), is associated with respiratory-related and death. Assays to detect virus-specific antibodies are important understand prevalence infection course immune response.Quantitative measurements plasma or serum nucleocapsid spike proteins were analyzed using luciferase immunoprecipitation system assays in 100 cross-sectional longitudinal samples from patients SARS-CoV-2 infection. A subset was tested...
Immune and inflammatory responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contribute disease severity of 2019 (COVID-19). However, the utility specific immune-based biomarkers predict clinical outcome remains elusive. Here, we analyzed levels 66 soluble in 175 Italian patients with COVID-19 ranging from mild/moderate critical assessed type I IFN–, II NF-κB–dependent whole-blood transcriptional signatures. A broad signature was observed, implicating activation various...
Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is a rare primary immunodeficiency disorder typically caused by homozygous
Patients with biallelic loss-of-function variants of AIRE suffer from autoimmune polyendocrine syndrome type-1 (APS-1) and produce a broad range autoantibodies (auto-Abs), including circulating auto-Abs neutralizing most type I interferons (IFNs). These were recently reported to account for at least 10% cases life-threatening COVID-19 pneumonia in the general population. We report 22 APS-1 patients 21 kindreds seven countries, aged between 8 48 yr infected SARS-CoV-2 since February 2020. The...
ABSTRACT Hepatitis C virus (HCV) and human pegivirus (HPgV or GB C) are globally distributed infect 2 to 5% of the population. The lack tractable-animal models for these viruses, in particular HCV, has hampered study infection, transmission, virulence, immunity, pathogenesis. To address this challenge, we searched homologous viruses small mammals, including wild rodents. Here report discovery several new hepaciviruses (HCV-like viruses) pegiviruses (GB virus-like that Complete genome...
ABSTRACT Genetic and biological characterization of new hepaciviruses infecting animals contributes to our understanding the ultimate origins hepatitis C virus (HCV) infection in humans dramatically enhances ability study its pathogenesis using tractable animal models. Animal homologs HCV include a recently discovered canine hepacivirus (CHV) GB B (GBV-B), both viruses with largely undetermined natural host ranges. Here we used versatile serology-based approach determine only known...
SARS-CoV-2, the cause of coronavirus disease 2019 (COVID-19), is associated with respiratory-related morbidity and mortality. Assays to detect virus-specific antibodies are important understand prevalence infection course immune response.Quantitative measurements plasma or serum by luciferase immunoprecipitation assay systems (LIPS) nucleocapsid spike proteins were analyzed in 100 cross-sectional longitudinal samples from SARS-CoV-2-infected patients. A subset was tested without heat...
Antibody detection of prior SARS-CoV-2 infection depends on severity, assay, and timing with implications for serosurveillance.
Abstract COVID-19 is known to cause multi-organ dysfunction 1-3 in acute infection, with prolonged symptoms experienced by some patients, termed Post-Acute Sequelae of SARS-CoV-2 (PASC) 4-5 . However, the burden infection outside respiratory tract and time viral clearance not well characterized, particularly brain 3,6-14 We performed complete autopsies on 44 patients map quantify distribution, replication, cell-type specificity across human body, including brain, from through over seven...
Post-infectious myalgic encephalomyelitis/chronic fatigue syndrome (PI-ME/CFS) is a disabling disorder, yet the clinical phenotype poorly defined, pathophysiology unknown, and no disease-modifying treatments are available. We used rigorous criteria to recruit PI-ME/CFS participants with matched controls conduct deep phenotyping. Among many physical cognitive complaints, one defining feature of was an alteration effort preference, rather than or central fatigue, due dysfunction integrative...
No conventional therapy exists for salivary hypofunction in surviving head and neck cancer patients with Radiation Therapy Oncology Group late grade 2-3 toxicity. We conducted a phase I clinical trial to test the safety biologic efficacy of serotype 5, adenoviral-mediated aquaporin-1 cDNA transfer single previously irradiated parotid gland 11 subjects using an open label, single-dose, dose-escalation design (AdhAQP1 vector; four dose tiers from 4.8 × 10(7) 5.8 10(9) vector particles per...
Volume 5, no. 2, doi:10.1128/mBio.00884-14, 2014. Author Peter D. Burbelo’s name was inadvertently omitted. The byline and affiliation line should appear as shown above. Also, the following statement be added to Acknowledgments:
Cyclin D1 is overexpressed in human tumors, correlating with cellular metastasis, and induced by activating Rho GTPases. Herein, cyclin D1-deficient mouse embryo fibroblasts (MEFs) exhibited increased adhesion decreased motility compared wild-type MEFs. Retroviral transduction of reversed these phenotypes. Mutational analysis demonstrated that its effects on migration were independent the pRb p160 coactivator binding domains. Genomewide expression arrays identified a subset genes regulated...
We investigated whether luciferase immunoprecipitation systems (LIPS) can be the basis for a more rapid, specific, and standardized assay diagnosis of Strongyloides stercoralis infection.A LIPS was developed based on immunoglobulin (Ig) G or IgG4 antibody to recombinant antigen (NIE) compared with an NIE enzyme-linked immunosorbent (ELISA). A second antigen, S. immunoreactive (SsIR), tested alone in combination NIE. The assays were using serum samples from patients parasitologically proven...