John S. Tsang
A5030115420- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- Immune responses and vaccinations
- Gene Regulatory Network Analysis
- Immune cells in cancer
- Bioinformatics and Genomic Networks
- Immune Response and Inflammation
- Diabetes and associated disorders
- Advanced Biosensing Techniques and Applications
- interferon and immune responses
- MicroRNA in disease regulation
- Respiratory viral infections research
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- CAR-T cell therapy research
- Kawasaki Disease and Coronary Complications
- Mitochondrial Function and Pathology
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
National Institutes of Health
2016-2025
National Institute of Allergy and Infectious Diseases
2016-2025
Center for Systems Biology
2014-2025
Yale University
2014-2025
Chan Zuckerberg Initiative (United States)
2025
Chan Zuckerberg Biohub New York
2025
Local Initiatives Support Corporation
2022
Center for Human Genetics
2022
ActionAid
2021
National Human Genome Research Institute
2020
Interindividual clinical variability in the course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is vast. We report that at least 101 987 patients with life-threatening disease 2019 (COVID-19) pneumonia had neutralizing immunoglobulin G (IgG) autoantibodies (auto-Abs) against interferon-ω (IFN-ω) (13 patients), 13 types IFN-α (36), or both (52) onset critical disease; a few also auto-Abs other three type I IFNs. The neutralize ability corresponding IFNs to block...
Immune and inflammatory responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contribute disease severity of 2019 (COVID-19). However, the utility specific immune-based biomarkers predict clinical outcome remains elusive. Here, we analyzed levels 66 soluble in 175 Italian patients with COVID-19 ranging from mild/moderate critical assessed type I IFN–, II NF-κB–dependent whole-blood transcriptional signatures. A broad signature was observed, implicating activation various...
SOMAscan is an aptamer-based proteomics assay capable of measuring 1,305 human protein analytes in serum, plasma, and other biological matrices with high sensitivity specificity. In this work, we present a comprehensive meta-analysis performance based on multiple serum plasma runs using the current 1.3 k assay, as well previous 1.1 version. We discuss normalization procedures examine different strategies to minimize intra- interplate nuisance effects. implement calibrator samples...
Glucocorticoids remain the most widely used immunosuppressive and anti-inflammatory drugs, yet substantial gaps exist in our understanding of glucocorticoid-mediated immunoregulation. To address this, we generated a pathway-level map transcriptional effects glucocorticoids on nine primary human cell types. This analysis revealed that response to is highly type dependent, terms individual genes pathways affected, as well magnitude direction regulation. Based these data given their importance...
Multimodal single-cell profiling methods that measure protein expression with oligo-conjugated antibodies hold promise for comprehensive dissection of cellular heterogeneity, yet the resulting counts have substantial technical noise can mask biological variations. Here we integrate experiments and computational analyses to reveal two major sources develop a method called "dsb" (denoised scaled by background) normalize denoise droplet-based data. We discover protein-specific originates from...
Several studies have shown that the pre-vaccination immune state is associated with antibody response to vaccination. However, generalizability and mechanisms underlie this association remain poorly defined. Here, we sought identify a common signature could predict across 13 different vaccines. Analysis of blood transcriptional profiles revealed three distinct endotypes, characterized by differential expression genes pro-inflammatory response, cell proliferation, metabolism alterations....
Naïve CD8 T cells have the potential to differentiate into a spectrum of functional states during an immune response. How these developmental decisions are made and what mechanisms exist suppress differentiation toward alternative fates remains unclear. We employed in vivo CRISPR-Cas9–based perturbation sequencing assess role ~40 transcription factors (TFs) epigenetic modulators cell fate decisions. Unexpectedly, we found that knockout TF Klf2 resulted aberrant exhausted-like acute...
Extremely premature infants (EPIs) who are born before 30 weeks of gestation susceptible to infection; however, the trajectory their peripheral immunity is poorly understood. Here, we undertook longitudinal analyses immune cells from 250 μl whole blood at 1 week, month, and 2 months 10 EPIs compared these with samples healthy adults preterm full-term cord samples. Single-cell suspensions individual were split perform single-cell RNA sequencing, T B cell receptor phosphoprotein mass...
Abstract Corticosteroids have been used for decades to modulate inflammation therapeutically, yet there is a paucity of data on their effects in humans. We examined the changes cellular and molecular immune system parameters, or “immunome”, healthy humans after systemic corticosteroid administration. multiplexed techniques query immunome 20 volunteers at baseline, intravenous hydrocortisone (HC) administered moderate (250 mg) low (50 doses, provide insight into how corticosteroids exert...
Annual influenza vaccinations are currently recommended for all individuals 6 months and older. Antibodies induced by vaccination an important mechanism of protection against infection. Despite the overall public health success vaccination, many fail to induce a substantial antibody response. Systems-level immune profiling studies have discerned associations between transcriptional cell subset signatures with responses. However, existing relied on small cohorts not been validated in large...