A5040049345- Immune cells in cancer
- Inflammation biomarkers and pathways
- Autoimmune and Inflammatory Disorders Research
- Otitis Media and Relapsing Polychondritis
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Bone Metabolism and Diseases
- Cell Image Analysis Techniques
- Kawasaki Disease and Coronary Complications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Phagocytosis and Immune Regulation
- Peroxisome Proliferator-Activated Receptors
- Immunotherapy and Immune Responses
- Medical Imaging Techniques and Applications
- Inflammatory mediators and NSAID effects
- AI in cancer detection
- Bone health and treatments
- NF-κB Signaling Pathways
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Cell death mechanisms and regulation
- Monoclonal and Polyclonal Antibodies Research
- Eosinophilic Disorders and Syndromes
- Image and Signal Denoising Methods
Universitätsklinikum Erlangen
2014-2025
Friedrich-Alexander-Universität Erlangen-Nürnberg
2014-2025
Imaging Center
2024
Rockefeller University
2023
National Institute of Allergy and Infectious Diseases
2016-2022
National Institutes of Health
2016-2022
Fraunhofer Institute for Cell Therapy and Immunology
2014
<h3>Objectives</h3> Autophagy is a homeostatic process to recycle dispensable and damaged cell organelles. Dysregulation of autophagic pathways has recently been implicated in the pathogenesis various diseases. Here, we investigated role autophagy during joint destruction arthritis. <h3>Methods</h3> osteoclasts was analysed vitro ex vivo by transmission electron microscopy, Western blotting immunohistochemistry for Beclin1 Atg7. Small molecule inhibitors, LysMCre-mediated knockout Atg7...
Abstract The intestinal microbiota influences mammalian host physiology in health and disease locally the gut but also organs devoid of direct contact with bacteria such as liver brain. Extracellular vesicles (EVs) or outer membrane (OMVs) released by microbes are increasingly recognized for their potential role biological shuttle systems inter‐kingdom communication. However, physiologically relevant evidence transfer functional biomolecules from to individual cells OMVs vivo is scarce. By...
Clodronate liposomes (Clo-Lip) have been widely used to deplete mononuclear phagocytes (MoPh) study the function of these cells in vivo. Here, we revisited effects Clo-Lip together with genetic models MoPh deficiency, revealing that exert their anti-inflammatory independent MoPh. Notably, not only but also polymorphonuclear neutrophils (PMN) ingested vivo, which resulted functional arrest. Adoptive transfer PMN, MoPh, reversed treatment, indicating stunning PMN rather than depletion accounts...
Objective To study whether Dickkopf (DKK)-1, an inhibitor of wingless (Wnt) signalling, is involved in the fusion sacroiliac joints. Methods Mice transgenic for tumour necrosis factor (TNFtg mice), which develop bilateral sacroiliitis, were treated with vehicle, anti-TNF antibody or anti-DKK1 antibody. Sacroiliac joints analysed histological signs inflammation, bone erosion, osteoclast formation and ankylosis. Moreover, expression collagen type X, β-catenin DKK-1 was assessed by...
Abstract Eicosanoids are essential mediators of the inflammatory response and contribute both to initiation resolution inflammation. Leukocyte-type 12/15-lipoxygenase (12/15-LO) represents a major enzyme involved in generation subclass eicosanoids, including anti-inflammatory lipoxin A4 (LXA4). Nevertheless, impact 12/15-LO on chronic diseases such as arthritis has remained elusive. By using two experimental models arthritis, K/BxN serum-transfer TNF transgenic mouse model, we show that...
Despite ongoing (macro)pinocytosis of extracellular fluid, the volume endocytic pathway remains unchanged. To investigate underlying mechanism, we used high-resolution video imaging to analyze fate macropinosomes formed by macrophages in vitro and situ. Na+, primary cationic osmolyte internalized, exited vacuoles via two-pore channels, accompanied parallel efflux Cl- osmotically coupled water. The resulting shrinkage caused crenation membrane, which fostered recruitment curvature-sensing...
Blood coagulation is essential for physiological hemostasis but simultaneously contributes to thrombotic disease. However, molecular and cellular events controlling initiation propagation of are still incompletely understood. In this study, we demonstrate an unexpected role eosinophils during plasmatic coagulation, hemostasis, thrombosis. Using a large-scale epidemiological approach, identified eosinophil cationic protein as independent predictive risk factor in humans. Concurrent...
During embryogenesis, the fetal liver becomes main hematopoietic organ, where stem and progenitor cells as well immature mature immune form an intricate cellular network. Hematopoietic (HSCs) reside in a specialized niche, which is essential for their proliferation differentiation. However, molecular determinants contributing to this HSC niche remain largely unknown. Macrophages are first differentiated found developing liver, they important erythropoiesis by promoting erythrocyte maturation...
DCs are able to undergo rapid maturation, which subsequently allows them initiate and orchestrate T cell-driven immune responses. DC maturation must be tightly controlled in order avoid random cell activation development of autoimmunity. Here, we determined that 12/15-lipoxygenase-meditated (12/15-LO-mediated) enzymatic lipid oxidation regulates fine-tunes consecutive Specifically, 12/15-LO activity the threshold via generation phospholipid products induced an antioxidative response...
Uptake of apoptotic cells (ACs) by macrophages ensures the nonimmunogenic clearance dying cells, as well maintenance self-tolerance to AC-derived autoantigens. Upon ingestion, ACs exert an inhibitory influence on inflammatory signaling within phagocyte. However, molecular signals that mediate these immune-modulatory properties are incompletely understood. In this article, we show phagocytosis thymocytes was enhanced in tissue-resident where process resulted inhibition NF-κB and repression...
Despite recent advances in treatment and vector control, malaria is still a leading cause of death, emphasizing the need for an effective vaccine. The life cycle can be subdivided into three stages: invasion growth within liver hepatocytes (pre-erythrocytic stage), blood stage (erythrocytic and, finally, sexual (occurring mosquito vector). Antigen (Ag)-specific CD8+ T cells are effectively induced by heterologous prime-boost viral immunization known to correlate with liver-stage protection....
Bleeding ulcers and erosions are hallmarks of active ulcerative colitis (UC). However, the mechanisms controlling bleeding mucosal haemostasis remain elusive.We used high-resolution endoscopy colon tissue samples UC (n = 36) as well experimental models physical chemical damage in mice deficient for peptidyl-arginine deiminase-4 (PAD4), gnotobiotic controls. We employed endoscopy, histochemistry, live-cell microscopy flow cytometry to study eroded surfaces during haemostasis.Erosions...
This article is part of the Dendritic Cell Guidelines series, which provides a collection state-of-the-art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization mouse human dendritic cells (DC) from lymphoid organs various non-lymphoid tissues. Here, we provide detailed procedures variety multiparameter microscopy imaging methods to explore spatial organization DC in tissues dissect how migrate, communicate,...
Abstract Efficient cellular fusion of mononuclear precursors is the prerequisite for generation fully functional multinucleated bone-resorbing osteoclasts. However, exact molecular factors and mechanisms controlling osteoclast remain incompletely understood. Here we identify RANKL-mediated activation caspase-8 as early key event during fusion. Single cell RNA sequencing-based analyses suggested that parts apoptotic machinery accompanied differentiation into mature A subsequent...