A5086828978- SARS-CoV-2 and COVID-19 Research
- Antifungal resistance and susceptibility
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Fungal Infections and Studies
- Immune Cell Function and Interaction
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Inhalation and Respiratory Drug Delivery
- Immune responses and vaccinations
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Transgenic Plants and Applications
- Viral gastroenteritis research and epidemiology
- Medical Device Sterilization and Disinfection
- Complement system in diseases
- Escherichia coli research studies
- Immune Response and Inflammation
- Respiratory Support and Mechanisms
- COVID-19 diagnosis using AI
- Single-cell and spatial transcriptomics
- Neurobiology and Insect Physiology Research
- vaccines and immunoinformatics approaches
- Plant Pathogens and Fungal Diseases
Innsbruck Medical University
2016-2025
Universität Innsbruck
2016-2025
Charité - Universitätsmedizin Berlin
2021
Ludwig-Maximilians-Universität München
2021
Institute of Virology of the Slovak Academy of Sciences
2021
Institute of Medical Microbiology and Hygiene
2015
University College London
2010
We introduce quanTIseq, a method to quantify the fractions of ten immune cell types from bulk RNA-sequencing data. quanTIseq was extensively validated in blood and tumor samples using simulated, flow cytometry, immunohistochemistry data.quanTIseq analysis 8000 revealed that cytotoxic T infiltration is more strongly associated with activation CXCR3/CXCL9 axis than mutational load deconvolution-based scores have prognostic value several solid cancers. Finally, we used show how kinase...
Different scenarios explaining the emergence of novel variants concern (VOC) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been reported, including their evolution in scarcely monitored populations, animals as alternative hosts, or immunocompromised individuals. Here we report SARS-CoV-2 immune escape mutations over a period seven months an patient with prolonged viral shedding. Signs infection, shedding and mutation events are periodically analyzed using RT-PCR...
Periodontal Ehlers-Danlos syndrome (pEDS) is an autosomal-dominant disorder characterized by early-onset periodontitis leading to premature loss of teeth, joint hypermobility, and mild skin findings. A locus was mapped approximately 5.8 Mb region at 12p13.1 but no candidate gene identified. In international consortium we recruited 19 independent families comprising 107 individuals with pEDS identify the locus, characterize clinical details in those defined genetic causes, try understand...
BackgroundExcessive inflammation triggered by a hitherto undescribed mechanism is hallmark of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections and associated with enhanced pathogenicity mortality.ObjectiveComplement hyperactivation promotes lung injury was observed in patients suffering from Middle East syndrome-related coronavirus, SARS-CoV-1, SARS-CoV-2 infections. Therefore, we investigated the very first interactions primary human airway epithelial cells on...
This article is part of the Dendritic Cell Guidelines series, which provides a collection state-of-the-art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization mouse human dendritic cells (DC) from lymphoid organs various non-lymphoid tissues. Here, we provide detailed procedures variety multiparameter microscopy imaging methods to explore spatial organization DC in tissues dissect how migrate, communicate,...
BackgroundToll-like receptors (TLRs) play a pivotal role in the immunologic response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Exaggerated inflammatory of innate immune cells, however, may drive morbidity and death Coronavirus disease 19 (COVID-19).ObjectiveWe investigated engagement SARS-CoV-2 with TLR4 order better understand how tackle hyperinflammation COVID-19.MethodsWe combined RNA-sequencing data human lung tissue bronchoalveolar lavage fluid cells...
To study interactions of airborne pathogens, e.g. Aspergillus (A.) fumigatus with upper and lower respiratory tract epithelial immune cells, we set up a perfused 3D human bronchial small airway cell system. Culturing normal or (NHBE, SAE) cells under air liquid interphase (ALI) perfusion resulted in significantly accelerated development the lung epithelia associated higher ciliogenesis, cilia movement, mucus-production improved barrier function compared to growth static conditions. Following...
Abstract Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance methods currently used. The participants comprised 12 laboratories from French university hospitals (nine them participating in Modimucor study) 11 Fungal PCR Initiative. For panel 1, three sera each spiked with DNA different species (Rhizomucor pusillus, Lichtheimia corymbifera, Rhizopus oryzae). 2, six concentrations R. pusillus L. corymbifera (1, 10, 100 genomes/ml)...
T cells play a fundamental role in the early control and clearance of many viral infections respiratory system. In SARS-CoV-2-infected individuals, lymphopenia with drastically reduced CD4+ CD8+ correlates Coronavirus disease 2019 (COVID-19)-associated severity mortality. this study, we characterized cellular humoral immune responses induced patients mild, severe critical COVID-19. Peripheral blood mononuclear 37 COVID-19 10 healthy individuals were analyzed by IFNγ ELISpot multi-color flow...
Few studies have directly compared virus-specific antibodies and their neutralizing capacity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) wild type (WT) circulating variants of concern despite the reported high efficacy messenger RNA (mRNA)- vector-based vaccines.
Abstract The COVID‐19 pandemic illustrated an urgent need for sophisticated, human tissue models to rapidly test and develop effective treatment options against this newly emerging severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Thus, in particular, the last 3 years faced extensive boost pulmonary model development. Nowadays, 3D models, organoids lung‐on‐chip, perfusion, or precision‐cut lung slices are used study complex research questions primary cells. These provide...
Abstract Upper respiratory tract infection (URTI) has a significant economic and social impact is major factor compromising athletes’ training competition. The effects of ColdZyme® Mouth Spray on URTI were investigated using an in vivo study athletes, combined with novel vitro air–liquid interface human airway model. Endurance athletes randomised to ColdZyme ( n = 78) or placebo 76) monitored over 3 months. They completed daily symptom logs collected throat swabs 7 days during perceived...
The polyene antifungal amphotericin B (AmB) is widely used to treat life-threatening fungal infections. Even though AmB resistance exceptionally rare in fungi, most Aspergillus terreus isolates exhibit an intrinsic against the drug vivo and vitro. Heat shock proteins perform a fundamental protective role multitude of stress responses, thereby maintaining protein homeostasis organism. In this study, we elucidated heat 70 (Hsp70) family members compared resistant susceptible A. clinical...
In this study, we characterize the impact of antioxidative enzymes in amphotericin B (AmB)-resistant (ATR) and rare AmB-susceptible (ATS) clinical Aspergillus terreus isolates. We elucidate expression profiles superoxide dismutase (SOD)- catalase (CAT)-encoding genes, enzymatic activities SODs, anion production signaling pathways involved oxidative stress response (OSR) ATS ATR strains under AmB treatment conditions. show that possess almost doubled basal SOD activity compared to exhibit an...
Infections with enterohemorrhagic Escherichia coli (EHEC) are a primary cause of hemolytic-uremic syndrome (HUS). Recently, Shiga toxin 2 (Stx2), the major virulence factor EHEC, was reported to interact complement, implying that latter is involved in pathogenesis EHEC-induced HUS. The aim present study investigate effect Stx2 on expression membrane-bound complement regulators CD46, CD55, and CD59 proximal tubular epithelial (HK-2) glomerular endothelial (GEnC) cells derived from human...
Anemia of chronic disease is a multifactorial disorder, resulting mainly from inflammation-driven reticuloendothelial iron retention, impaired erythropoiesis, and reduced biological activity erythropoietin. Erythropoiesis-stimulating agents have been used for the treatment anemia disease, although with varying response rates potential adverse effects. Serum concentrations hepcidin, key regulator homeostasis, are increased in patients linked to pathogenesis this because hepcidin blocks...
DCs express intrinsic cellular defense mechanisms to specifically inhibit HIV-1 replication. Thus, are productively infected only at very low levels with HIV-1, and this non-permissiveness of is suggested go along viral evasion. We now illustrate that complement-opsonized (HIV-C) efficiently bypasses SAMHD1 restriction infects including BDCA-1 DCs. Efficient DC infection by HIV-C was also observed using single-cycle HIV-C, correlated a remarkable elevated T592 phosphorylation but not...
Amongst the many strategies aiming at inhibiting HIV-1 infection, blocking viral entry has been recently recognized as a very promising approach. Using diverse in vitro models and broad range of primary patient isolates, we report here that IND02, type A procyanidin polyphenol extracted from cinnamon, features trimeric pentameric forms displays an anti-HIV-1 activity against CXCR4 CCR5 viruses with 1–7 μM ED50 for trimer. Competition experiments, using surface plasmon resonance-based binding...
Small, cysteine-rich and cationic antimicrobial proteins (AMPs) from filamentous ascomycetes represent ideal bio-molecules for the development of next-generation antifungal therapeutics. They are promising candidates to counteract resistance may complement or even replace current small molecule-based antibiotics in future. In this study, we show that a 14 amino acid (aa) long peptide (Pγ) spanning highly conserved γ-core motif Penicillium chrysogenum protein (PAF) has activity against...
Candidemia poses a major threat to ICU patients and is routinely diagnosed by blood culture, which known for its low sensitivity long turnaround times. We compared the performance of novel, Candida-specific multiplex real-time PCR assay (Fungiplex® Candida IVD Real-Time Kit) with culture another established diagnostic (LightCycler SeptiFast Test) respect detection from whole samples. Clinical samples 58 were analyzed standard (BC) simultaneously tested Fungiplex (FP) test (SF) molecular spp....