A5025368461- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Hepatitis B Virus Studies
- Syphilis Diagnosis and Treatment
- Animal Virus Infections Studies
- Reproductive tract infections research
- Respiratory viral infections research
- SARS-CoV-2 detection and testing
- HIV, Drug Use, Sexual Risk
- HIV-related health complications and treatments
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Pneumonia and Respiratory Infections
- Long-Term Effects of COVID-19
- Influenza Virus Research Studies
- Bacterial Genetics and Biotechnology
- Computational Drug Discovery Methods
- Hepatitis Viruses Studies and Epidemiology
- interferon and immune responses
- Herpesvirus Infections and Treatments
National Taiwan University
2016-2025
National Taiwan University Hospital
2016-2025
Mackay Memorial Hospital
2014-2024
University of California, Davis
2024
Taipei Medical University
2007-2022
Genomics Research Center, Academia Sinica
2020
Industrial Technology Research Institute
2012-2020
Institute of Biomedical Sciences, Academia Sinica
2020
Pharmaceutical Biotechnology (Czechia)
2016
National Yang Ming Chiao Tung University
2004-2015
Methionine aminopeptidase (MAP) catalyzes the removal of amino-terminal methionine from proteins. The Escherichia coli map gene encoding this enzyme was cloned; it consists 264 codons and encodes a monomeric 29,333 daltons. In vitro analyses with purified indicated that MAP is metallo-oligopeptidase absolute specificity for methionine. residues end recombinant proteins interleukin-2 (Met-Ala-Pro-IL-2) ricin A (Met-Ile-Phe-ricin A) could be removed either in or vivo MAP-hyperproducing strains...
Development of specific antiviral agents is an urgent unmet need for SARS-coronavirus 2 (SARS-CoV-2) infection. This study focuses on host proteases that proteolytically activate the SARS-CoV-2 spike protein, critical its fusion after binding to angiotensin-converting enzyme (ACE2), as targets. We first validate cleavage at a putative furin substrate motif spikes by expressing it in VeroE6 cells and find prominent syncytium formation. Cleavage are abolished treatment with inhibitors...
A major challenge to end the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is develop a broadly protective vaccine that elicits long-term immunity. As key immunogen, viral surface spike (S) protein frequently mutated, and conserved epitopes are shielded glycans. Here, we revealed S glycosylation has site-differential effects on infectivity. We found generated lung epithelial cells glycoforms associated with increased Compared fully glycosylated protein,...
The Thermus aquaticus DNA polymerase I (Taq Pol I) gene was cloned into a plasmid expression vector that utilizes the strong bacteriophage lambda PL promoter. A truncated form of Taq also constructed. two constructs made it possible to compare full-length 832-amino-acid and deletion derivative encoding 544-amino-acid translation product, Stoffel fragment. Upon heat induction, construct produced 1-2% total protein as I. induced 3% Enzyme purification included cell lysis, treatment followed by...
We localized the methionine aminopeptidase (map) gene on Escherichia coli chromosome next to rpsB at min 4. Genetically modified strains with chromosomal map under lac promoter control grew only in presence of operon inducer isopropyl-beta-thiogalactoside. Thus, is essential for cell growth.
The elicitation of large amount inflammatory cytokine in serum has been developed as the cause plasma leakage dengue fever (DF)/dengue haemorrhagic (DHF) infection. Virus recognition innate immunity is key. Toll-like receptors (TLRs) play an important role pathogen towards induction among several viruses; however, TLRs on immune against DENV remains unclear. This study aims at interaction between virus (DENV) and human incipient stage infection vitro. Our experiment reveals that stably...
A positive retroregulator that enhances the expression of an upstream gene(s) has been identified. It resides within a 381-base pair (bp) restriction fragment containing transcriptional terminator crystal protein (cry) gene from Bacillus thuringiensis vs. Kurstaki HD-1. This was fused to distal ends either penicillinase (penP) licheniformis or interleukin 2 cDNA human Jurkat cell line. In both cases, half-lives mRNAs derived fusion genes were increased approximately equal 6 min in...
Abstract Background SARS-CoV-2 began spreading in December 2019 and has since become a pandemic that impacted many aspects of human society. Several issues concerning the origin, time introduction to humans, evolutionary patterns, underlying force driving outbreak remain unclear. Method Genetic variation 137 genomes related coronaviruses as 2/23/2020 was analyzed. Result After correcting for mutational bias, excess low frequency mutations on both synonymous nonsynonymous sites revealed which...
The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants has altered the trajectory COVID-19 pandemic and raised some uncertainty on long-term efficiency vaccine strategy. development new therapeutics against a wide range SARS-CoV-2 is imperative. We, here, have designed an inhalable siRNA, C6G25S, which covers 99.8% current capable inhibiting dominant strains, including Alpha, Delta, Gamma, Epsilon, at picomolar ranges IC50 in vitro. Moreover, C6G25S could...
ABSTRACT An evolving dominance of human immunodeficiency virus type 1 subtype C (HIV-1C) in the AIDS epidemic has been associated with a high prevalence HIV-1C infection southern African countries and an expanding India China. Understanding molecular phylogeny genetic diversity viruses may be important for design evaluation HIV vaccine ultimate use developing world. In this study we analyzed phylogenetic relationships (i) between 73 nonrecombinant near-full-length genome sequences, including...
The chromosomal beta-lactamase (penicillinase, penP) gene from Bacillus licheniformis 749/C has been cloned in Escherichia coli. locations of the target sites for various restriction enzymes on 4.2-kilobase EcoRI fragment were determined. By matching mapping data with potential nucleotide sequences penP deduced known protein sequence, we established exact position fragment. A bifunctional plasmid vector carrying gene, pOG2165, was constructed which directs synthesis heterologous both E. coli...
The nucleotide sequence of the coding and regulatory regions alpha-amylase gene (aml) Streptomyces limosus was determined. High-resolution S1 mapping used to locate 5' end transcript demonstrated that is transcribed from a unique promoter. predicted amino acid has considerable identity mammalian invertebrate alpha-amylases, but not those plant, fungal, or eubacterial origin. Consistent with this susceptibility enzyme an inhibitor alpha-amylases. amino-terminal extracellular determined,...
The nucleotide sequence of the prolipoprotein signal peptidase (lsp) gene has been determined. lsp was found to be adjacent isoleucyl-tRNA synthetase ( ileS ) gene, such that termination codon overlaps with initiation lsp. These two genes are transcribed in same direction and major promotor for appears upstream . Identification established by amplification activity strains carrying a subcloned 1.1-kilobase Stu I-Acc I fragment further confirmed introducing mutational alterations COOH...
Incidence of Entamoeba histolytica infection and clinical manifestations treatment response invasive amebiasis (IA) in HIV-infected patients have rarely been investigated before.At the National Taiwan University Hospital, medical records who received a diagnosis IA between 1994 2005 were reviewed. The incidence was serial blood stool samples from 670 264 patients, respectively, using serological specific amebic antigen assays. DNA extracted containing E. analyzed by PCR, sequenced, compared....
Site-specific genetic recombinations promoted in vivo by the EcoRI endonuclease has been demonstrated using constructed hybrid plasmids which chloramphenicol resistance gene was inactivated insertion of DNA fragments at an site within gene. Such recombination can involve either joining intracellularly generated cohesive termini same fragment or intermolecular ligation different fragments. cleavage and are precise: recombinant molecules show functional continuity sequence cleaved enzyme...
Herpes simplex virus (HSV), a common latent in humans, causes certain severe diseases. Extensive use of acyclovir (ACV) results the development drug-resistant HSV strains, hence, there is an urgent need to develop new drugs treat infection. Houttuynia cordata (H. cordata), natural herbal medicine, has been reported exhibit anti-HSV effects which partly NF-κB-dependent. However, molecular mechanisms by H. inhibits infection are not elucidated thoroughly. Here, we report that water extracts...
To circumvent the devastating pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, a humanized decoy antibody (ACE2-Fc fusion protein) was designed to target interaction between viral spike protein and its cellular receptor, angiotensin-converting enzyme (ACE2). First, we demonstrated that ACE2-Fc could specifically abrogate virus replication blocking entry of SARS-CoV-2 spike-expressing pseudotyped into both ACE2-expressing lung cells organoids. The...
Coronavirus (CoV) disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has claimed many lives worldwide and is still spreading since December 2019. The 3C-like protease (3CL pro ) papain-like (PL are essential for maturation of viral polyproteins in SARS-CoV-2 life cycle thus regarded as key drug targets disease.