A5054716842- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Angiogenesis and VEGF in Cancer
- Lung Cancer Research Studies
- Gene expression and cancer classification
- Traditional Chinese Medicine Analysis
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Immune cells in cancer
- RNA Research and Splicing
- Lung Cancer Diagnosis and Treatment
- 14-3-3 protein interactions
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Heat shock proteins research
- Plant Molecular Biology Research
- Cell Adhesion Molecules Research
- Gastric Cancer Management and Outcomes
- Computational Drug Discovery Methods
National Chung Hsing University
2016-2025
Peking Union Medical College Hospital
2022-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2023
National Yang Ming Chiao Tung University
2006-2022
Institute of Biomedical Science
2006-2022
National Taipei University
2004-2021
National Taiwan University Hospital
2000-2017
Renji Hospital
2017
Taichung Veterans General Hospital
2006-2016
Institute of Statistical Science, Academia Sinica
2016
Current staging methods are inadequate for predicting the outcome of treatment non–small-cell lung cancer (NSCLC). We developed a five-gene signature that is closely associated with survival patients NSCLC.
Inflammation plays a critical role in cancer progression. In this study we investigate the pro-tumorigenic activities and gene expression profiles of lung cells after interaction with macrophages.We measured intratumoral microvessel counts macrophage density 41 tumor specimens correlated these patients' clinical outcome. The between macrophages cell lines was assessed using transwell coculture system. invasive potential evaluated by vitro invasion assay. matrix-degrading activity assayed...
Abstract Macrophages in a tumor microenvironment have been characterized as M1- and M2-polarized subtypes. Here, we discovered the different macrophages’ impacts on lung cancer cell A549. The M2a/M2c subtypes promoted A549 invasion xenograft growth. M1 subtype suppressed angiogenesis. enhanced sensitivity of to cisplatin decreased tube formation activity viability cells by inducing apoptosis senescence. Different macrophage regulated genes involved immune response, cytoskeletal remodeling,...
This work introduces Gemma, a family of lightweight, state-of-the art open models built from the research and technology used to create Gemini models. Gemma demonstrate strong performance across academic benchmarks for language understanding, reasoning, safety. We release two sizes (2 billion 7 parameters), provide both pretrained fine-tuned checkpoints. outperforms similarly sized on 11 out 18 text-based tasks, we present comprehensive evaluations safety responsibility aspects models,...
Titanium dioxide nanoparticles (nanoTiO2) have been widely used as a photocatalyst in air and water cleaning. However, these inhalation can induce pulmonary toxicity its mechanism is not fully understood. In this study we investigated the of nanoTiO2 molecular pathogenesis. The adult male ICR mice were exposed to intratracheal single dose 0.1 or 0.5 mg (19-21 nm) lung tissues collected at 3rd day, 1st wk, 2nd wk for morphometric, microarray gene expression, pathway analyses. NanoTiO2...
Abstract Curcumin (diferuloylmethane) is an active component of the spice turmeric and has a diversity antitumor activities. In this study, we found that curcumin can inhibit cancer cell invasion metastasis through activation tumor suppressor DnaJ-like heat shock protein 40 (HLJ1). Human lung adenocarcinoma cells (CL1-5) treated with (1–20 μmol/L) showed concentration-dependent reduction in migration, invasion, metastatic ability, was associated increased HLJ1 expression. Knockdown...
Abstract Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cells. Here, investigated clinical significance of lung adenocarcinoma and role process metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate mRNA surgically resected 54 patients its correlation with survival. We line very low levels vitro vivo...
Abstract The NAD(P)H dehydrogenase complex is encoded by 11 ndh genes in plant chloroplast (cp) genomes. However, are truncated or deleted some autotrophic Epidendroideae orchid cp To determine the evolutionary timing of gene deletions and genomic locations various orchids, genomes Vanilla planifolia , Paphiopedilum armeniacum niveum Cypripedium formosanum Habenaria longidenticulata Goodyera fumata Masdevallia picturata were sequenced; these represent Vanilloideae, Cypripedioideae,...
Angiogenesis is a hallmark of cancer and plays critical role in lung progression, which involves interactions between cells, endothelial cells the surrounding microenvironment. However, gene expression profiles changes biological phenotype vascular after with remain unclear. An indirect transwell co-culture system was used to survey interaction human umbilical vein (HUVECs) adenocarcinoma CL1-5 as well investigate morphological molecular HUVECs. The differentially expressed genes (DEGs)...
The chloroplast NAD(P)H dehydrogenase-like (NDH) complex consists of about 30 subunits from both the nuclear and genomes is ubiquitous across most land plants. In some orchids, such as Phalaenopsis equestris, Dendrobium officinale catenatum, 11 genome-encoded ndh genes (cp-ndh) have been lost. Here we investigated whether functional cp-ndh completely lost in these orchids or they transferred retained genome. Further, assessed nucleus-encoded NDH-related can be lost, resulting absence NDH...
Background It is important to select appropriate targeted therapies for subgroups of patients with lung adenocarcinoma who have specific gene alterations. Methods This prospective study was a multicenter project conducted in Taiwan assessment genetic tests. Five oncogenic drivers, including EGFR, KRAS, BRAF, HER2 and EML4-ALK fusion mutations, were tested. BRAF mutations assessed by MALDI-TOF MS (Cohort 1). translocation tested Ventana method EGFR-wild type 2). Results From August 2011...
The DiseaseConnect (http://disease-connect.org) is a web server for analysis and visualization of comprehensive knowledge on mechanism-based disease connectivity. traditional classification system groups diseases with similar clinical symptoms phenotypic traits. Thus, entirely different pathologies could be grouped together, leading to treatment design. Such problems avoided if were classified based their molecular mechanisms. Connecting pathological mechanisms inspire novel strategies the...
Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel lung cell lines (CL1–0, CL1–1, CL1–5, CL1–5-F4 in order increasing invasive activity) for such genes selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used microarray containing 9600 gene sequences assess expression the CRMP-1 further study. confirmed differential...
Purpose This study was conducted to characterize gene expression profile of survival in patients with surgically curable gastric cancer by using an in-house membrane microarray and developing a prediction model. Materials Methods Data cDNA microarrays were obtained from 18 pairs cancerous noncancerous tissues. Nine who survived > 30 months identified as good survival, the other nine, < 12 months, poor survival. Supervised analysis performed identify Semiquantitative reverse...
Abstract Tanshinones are the major bioactive compounds of Salvia miltiorrhiza Bunge (Danshen) roots, which used in many therapeutic remedies Chinese traditional medicine. We investigated anticancer effects tanshinones on highly invasive human lung adenocarcinoma cell line, CL1-5. Tanshinone I significantly inhibited migration, invasion, and gelatinase activity macrophage-conditioned medium-stimulated CL1-5 cells vitro also reduced tumorigenesis metastasis CL1-5-bearing severe combined...
We previously identified DnaJ-like heat shock protein (HLJ1) as a gene associated with tumor invasion. Here, we investigated the clinical significance of HLJ1 expression in non-small-cell lung cancer (NSCLC) patients and its role progression.We induced overexpression or knockdown human adenocarcinoma CL1-5 cells analyzed cell proliferation, anchorage-independent growth, vivo tumorigenesis, motility, invasion, cycle progression. Expression genes that act downstream was examined by DNA...
Background Non-small cell lung cancer (NSCLC) patients with L858R or exon 19 deletion mutations in epidermal growth factor receptor (EGFR) have good responses to the tyrosine kinase inhibitor (TKI), gefitinib. However, wild-type EGFR and acquired mutation T790M are resistant gefitinib treatment. Here, we showed that curcumin can improve efficiency of NSCLC cells both vitro vivo models. Methods/Principal Findings After screening 598 herbal natural compounds, found could inhibit proliferation...
Key innovations have facilitated novel niche utilization, such as the movement of algal predecessors land plants into terrestrial habitats where drastic fluctuations in light intensity, ultraviolet radiation and water limitation required a number adaptations. The NDH (NADH dehydrogenase-like) complex Viridiplantae plastids participates adapting photosynthetic response to environmental stress, suggesting its involvement transition habitats. Although relatively rare, loss or pseudogenization...