A5032742125- Angiogenesis and VEGF in Cancer
- Pleural and Pulmonary Diseases
- Lung Cancer Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Trauma Management and Diagnosis
- Immune cells in cancer
- Ultrasound in Clinical Applications
- RNA modifications and cancer
- Cardiac Arrest and Resuscitation
- Pneumothorax, Barotrauma, Emphysema
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- MRI in cancer diagnosis
- Cancer-related Molecular Pathways
- Axon Guidance and Neuronal Signaling
- Cancer Cells and Metastasis
- Tracheal and airway disorders
- Radiomics and Machine Learning in Medical Imaging
- Chemokine receptors and signaling
- Glycosylation and Glycoproteins Research
- Congenital Diaphragmatic Hernia Studies
- Ultrasound and Hyperthermia Applications
- Sepsis Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Ferroptosis and cancer prognosis
The Fourth People's Hospital of Ningxia Hui Autonomous Region
2024
Hospital Kuala Lumpur
2024
National Taiwan University Hospital
2006-2017
National Taiwan University
2003-2017
National Cheng Kung University
2006
Center for Genomic Science
2005
National Chung Hsing University
2004-2005
National Health Research Institutes
2005
Institute of Biomedical Sciences, Academia Sinica
1995-2005
National Taipei University
2004
Current staging methods are inadequate for predicting the outcome of treatment non–small-cell lung cancer (NSCLC). We developed a five-gene signature that is closely associated with survival patients NSCLC.
Inflammation plays a critical role in cancer progression. In this study we investigate the pro-tumorigenic activities and gene expression profiles of lung cells after interaction with macrophages.We measured intratumoral microvessel counts macrophage density 41 tumor specimens correlated these patients' clinical outcome. The between macrophages cell lines was assessed using transwell coculture system. invasive potential evaluated by vitro invasion assay. matrix-degrading activity assayed...
Abstract Macrophages in a tumor microenvironment have been characterized as M1- and M2-polarized subtypes. Here, we discovered the different macrophages’ impacts on lung cancer cell A549. The M2a/M2c subtypes promoted A549 invasion xenograft growth. M1 subtype suppressed angiogenesis. enhanced sensitivity of to cisplatin decreased tube formation activity viability cells by inducing apoptosis senescence. Different macrophage regulated genes involved immune response, cytoskeletal remodeling,...
The purpose of this study was to evaluate the correlation between expression four different vascular endothelial growth factor (VEGF) mRNA isoforms (VEGF121, VEGF165, VEGF 189, and VEGF206) clinicopathologic characteristics, tumor angiogenesis, outcome patients with non-small-cell lung cancer.We examined in 57 cancers using reverse transcriptase polymerase chain reaction angiogenesis immunohistochemical staining.All cancer samples expressed VEGF121, VEGF189 isoforms, three VEGF206 isoform. A...
Abstract Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cells. Here, investigated clinical significance of lung adenocarcinoma and role process metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate mRNA surgically resected 54 patients its correlation with survival. We line very low levels vitro vivo...
Neuropilin 1 (NRP1) is a mediator of lung branching and angiogenesis in embryonic development cancer. The role NRP1 cancer progression not fully elucidated. We investigated the invasion tumor angiogenesis, its signaling pathways, prognostic significance, therapeutic implications.Sixty patients with non-small cell (NSCLC) were studied. mRNA expression was measured using real-time quantitative reverse-transcription PCR. invasion, pathways studied stimulation by vascular endothelial growth...
Angiogenesis is a hallmark of cancer and plays critical role in lung progression, which involves interactions between cells, endothelial cells the surrounding microenvironment. However, gene expression profiles changes biological phenotype vascular after with remain unclear. An indirect transwell co-culture system was used to survey interaction human umbilical vein (HUVECs) adenocarcinoma CL1-5 as well investigate morphological molecular HUVECs. The differentially expressed genes (DEGs)...
Tumor-associated angiogenesis is important for tumor growth and metastasis. Interleukin (IL)-8 was recently reported to be an angiogenic factor both in vitro vivo. In this study we evaluated, the first time, IL-8 messenger RNA (mRNA) expression non–small-cell lung cancer (NSCLC), using real-time quantitative reverse-transcription–polymerase chain reaction, correlated mRNA nontumor samples from 58 patients with NSCLC (29 squamous cell carcinoma 29 adenocarcinoma, of whom 20 had Stage I, 10...
Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel lung cell lines (CL1–0, CL1–1, CL1–5, CL1–5-F4 in order increasing invasive activity) for such genes selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used microarray containing 9600 gene sequences assess expression the CRMP-1 further study. confirmed differential...
This study proposed to evaluate the efficacy of color Doppler sonography in detecting possible differences blood flow patterns between malignant and benign cervical lymph nodes.During a period 12 months, palpable nodes 48 untreated patients were prospectively evaluated with wave analysis. Histopathologic diagnoses obtained by sonographically guided fine-needle aspiration biopsy and/or excisional biopsy.We found 16 (four tuberculous lymphadenitis, four reactive hyperplasia, eight unspecified)...
We have quantified the expression of all 4 isoforms vascular endothelial growth factor (VEGF) mRNA in non-small-cell lung cancer (NSCLC) using a new kinetic quantitative PCR method, real-time (RTQ) RT-PCR, and investigated association between VEGF at protein levels clinicopathologic variables, tumor angiogenesis, patient survival timing relapse. Surgical specimens from 72 NCSLC patients (37 squamous-cell carcinomas, 35 adenocarcinomas) were examined. Twenty-eight had stage I, 10 II 34 IIIA...
PURPOSE: To evaluate interactions between expressions of tumor suppressor gene p53 and angiogenic factors vascular endothelial cell growth factor (VEGF) interleukin-8 (IL-8) their effect on angiogenesis patient prognosis in non–small-cell lung cancer (NSCLC). PATIENTS AND METHODS: p53, VEGF, IL-8, the microvessel endothelium were immunostained, VEGF IL-8 mRNA expression quantified using real-time quantitative reverse-transcription polymerase chain reaction 65 NSCLC surgical specimens....
This study investigated the clinico-pathologic correlation of tumor angiogenesis in non-small-cell lung cancers. Formalin-fixed, paraffin-embedded surgical specimens 55 consecutive patients with primary cancers were examined. Included 26 squamous cell carcinomas and 29 adenocarcinomas. Twenty-five had stage I disease, eight II 22 IIIA or IIIB disease. Among them, 28 nodal metastasis 27 did not. The microvessel was demonstrated by immunocytochemical staining for factor VIII platelet...
Cardiac troponin I (cTnI) is a specific marker of myocardial damage used in the diagnosis acute coronary syndrome (ACS). Recent studies have shown that cTnI levels can also be elevated patients without ACS, such as sepsis and trauma patients, this associated with an adverse prognosis. We evaluated clinical implications prognostic significance serum noncardiac critically ill prospective observational study general medical intensive care unit at tertiary-level hospital. A total 108 consecutive...
The purpose of this study was to compare blood flow in benign and malignant pulmonary masses by assessing the spectral waveforms obtained during color Doppler sonography. Fifty lung cancers 46 patients 28 lesions were evaluated with Spectral wave analysis (specifically, variables pulsatility index, resistive peak systolic velocity, end diastolic velocity) used determine distal impedance vessels lesions. signal detection rates for 64% 79%, respectively. All detectable signals had either...
Data obtained from 1996 to 2002 on 13 patients with Rhizobium radiobacter infections were analyzed. Ten (76%) had underlying hematological malignancy or solid-organ cancer. Six (46%) febrile neutropenia during the course of R. infection. The majority (54%) catheter-related bacteremia, and 92% hospital acquired. All survived. Eighteen isolates recovered patients, each isolate was susceptible cefepime, piperacillin-tazobactam, carbapenems, ciprofloxacin. pulsed-field gel electrophoresis...
To assess the value of color Doppler ultrasound in distinguishing minimal pleural effusion from thickening, a prospective analysis was done on ultrasonographic findings 51 patients. Real-time, gray-scale, and chest examinations were carried out by different sonographers who had no clinical information concerning The sonographer evaluated images for internal echogenicity effusion, lesions that change shape with respiration, movable septa echo-densities space conventional gray-scale as well...
Cancer metastasis is not exclusively regulated by the deregulation of metastasis-promoting or suppressing genes in cancer cells. The interaction between cells and stromal has been shown recently to promote metastasis. macrophages within tumor, referring as tumour-associated (TAMs), are pivotal member TAMs derived from peripheral blood monocytes recruited into tumor. Upon activated cells, can release a vast diversity growth factors, proteolytic enzymes, cytokines, inflammatory mediators. Many...
Interaction between cancer cells and adjacent stromal is important to promote tumor development. Our aim was study total COX-2 mRNA expression in both surrounding its association with angiogenic factor VEGF expression, angiogenesis prognosis patients NSCLC. tissue analyzed using real-time quantitative (RTQ) RT-PCR 60 NSCLC surgical specimens. Immunohistochemistry (IHC) used localize protein Correlations tumoral (measured by RTQ RT-PCR), intratumoral microvessel counts (evaluated IHC), other...