A5024331756- Connective Tissue Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Inflammatory mediators and NSAID effects
- Cancer-related gene regulation
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Histone Deacetylase Inhibitors Research
- Estrogen and related hormone effects
- S100 Proteins and Annexins
- Mechanisms of cancer metastasis
- RNA modifications and cancer
- Connective tissue disorders research
- Glutathione Transferases and Polymorphisms
- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Fibroblast Growth Factor Research
- Peptidase Inhibition and Analysis
- Cancer therapeutics and mechanisms
- Lymphatic System and Diseases
- TGF-β signaling in diseases
Chang Gung University
2024
National Taiwan University
2010-2023
Institute of Pharmacology
2008-2023
Taipei Veterans General Hospital
1992-2020
Shizuoka Institute of Science and Technology
2019
Weatherford College
2019
Kaohsiung Medical University
2010-2017
Fuzhou University
2016
Kaohsiung Medical University Chung-Ho Memorial Hospital
2010-2015
Mackay Memorial Hospital
2015
Patients with non-small-cell lung cancer (NSCLC) epidermal growth factor receptor (EGFR)-activating mutations have excellent response to EGFR tyrosine kinase inhibitors (TKIs), but T790M mutation accounts for most TKI drug resistance. This study used highly sensitive methods detect before and after therapy investigated the association of its frequencies clinical outcome.Direct sequencing, matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) next-generation...
Abstract G9a is a mammalian histone methyltransferase that contributes to the epigenetic silencing of tumor suppressor genes. Emerging evidence suggests required maintain malignant phenotype, but role function in mediating metastasis has not been explored. Here, we show expressed aggressive lung cancer cells, and its elevated expression correlates with poor prognosis. RNAi-mediated knockdown highly invasive cells inhibited cell migration invasion vitro vivo. Conversely, ectopic weakly...
Abstract Reports of the bone-protective effects resveratrol, a naturally occurring phytoestrogen and agonist for longevity gene SIRT1, have highlighted this compound as candidate therapy osteoporosis. Moreover, SIRT1 antagonism enhances adipogenesis. There has been speculation that resveratrol can promote osteogenesis through but mechanism remains unclear. In study we investigated molecular how modulate lineage commitment human mesenchymal stem cells to other than We found promoted...
mcl-1 is an immediate-early gene activated by the granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (IL-3) signaling pathways plays important role in viability response of these cytokines. In this study, we demonstrated that cytokine stimulation mRNA protein expression were attenuated pretreatment cells with phosphatidylinositol 3-kinase (PI3-K) inhibitors. Reporter assays further showed PI3-K/Akt pathway was involved IL-3 activation transcription. Analysis promoter...
The multifunctional cytokine interleukin-6 (IL-6) regulates growth and differentiation of many cell types induces production acute-phase proteins in hepatocytes. Here we report that IL-6 protects hepatoma cells from apoptosis induced by transforming factor-β (TGF-β), a well known apoptotic inducer liver cells. Addition blocked TGF-β-induced activation caspase-3 while showing no effect on the induction plasminogen activator inhibitor-1 p15<sup>INK4B</sup> genes, indicating interferes with...
Resveratrol, a polyphenolic compound found in grapes and other fruits, has been reported to inhibit angiogenesis with an as yet elusive mechanism. Here, we investigate the detailed mechanism by which resveratrol inhibits vascular endothelial growth factor (VEGF)-induced angiogenic effects human umbilical cells (HUVECs). Exposure of HUVECs 1 2.5 μM significantly blocked VEGF-mediated migration tube formation but not cell proliferation. Under same concentrations, failed affect VEGF-stimulated...
The aggressiveness of a tumor is partly attributed to its resistance chemotherapeutic agent-induced apoptosis. Cysteine-rich 61 (Cyr61), from the CCN gene family, secreted and matrix-associated protein, which involved in many cellular activities such as growth differentiation. Here we established cell model system examine whether stable expression Cyr61 MCF-7 cells can confer apoptosis identify possible participating mechanisms. We showed that lines overexpressing had acquired remarkable...
Hypoxia-inducible factor-1alpha (HIF-1alpha) is widely considered to be one of the key regulators tumor angiogenesis. The upstream regulation complex and involves several growth factors, cytokines, hypoxia. Herein, we have identified miR-519c as a hypoxia-independent regulator HIF-1alpha, acting through direct binding HIF-1alpha 3' untranslated region leading reduced Overexpression resulted in significant decrease protein levels tube formation human umbilical vein endothelial cells;...
mcl-1, a bcl-2 family member, was originally identified as an early gene induced during differentiation of ML-1 myeloid leukemia cells. In the present study, we demonstrate that Mcl-1 is tightly regulated by granulocyte-macrophage colony-stimulating factor (GM-CSF) signaling pathway. Upon deprivation survival from TF-1 progenitor cells, levels quickly dropped prior to visible detection apoptosis these restimulation deprived cells with GM-CSF, mcl-1 mRNA immediately and its protein product...
Abstract Cyclooxygenase (COX)-2, the inducible isoform of prostaglandin H synthase, has been implicated in progression human lung adenocarcinoma. However, mechanism underlying COX-2’s effect on tumor remains largely unknown. Lymphangiogenesis, formation new lymphatic vessels, recently received considerable attention and become a frontier metastasis research. Here, we study interaction between COX-2 lymphangiogenic factor, vascular endothelial growth factor (VEGF)-C, cancer cells their...
Classic signaling by the proinflammatory cytokine interleukin 6 (IL-6) involves its binding to target cells that express membrane-bound IL-6 receptor α. However, an alternate pathway exists in which soluble (sIL-6Rα) can bind and activate lack mIL-6Rα, such as endothelial cells. This pathway, also termed trans-signaling, serves major various pathologic conditions including cancer. Here we report sIL-6Rα is elevated malignant ascites from ovarian cancer patients, where it associated with poor...
Gastric carcinoma is one of the most common cancers and lethal malignancies worldwide. Thus far, regulatory mechanisms its aggressiveness are still poorly understood. To understand pathogenesis to develop new therapeutic strategies, it essential dissect molecular that regulate progression gastric cancer. Herein, we sought address whether Notch1 signal pathway involved in control We found expression Notch ligand Jagged1 was correlated with human Patients cancer tissues had a poor survival...
Ovarian cancer (OCa) peritoneal metastasis is the leading cause of cancer–related deaths in women with limited therapeutic options available for treating it and poor prognosis, as underlying mechanism not fully understood. The clinicopathological correlation G9a expression was assessed tumor specimens ovarian patients. Knockdown or overexpression cell lines analysed regard to its effect on adhesion, migration, invasion anoikis-resistance. In vivo biological functions were tested by i.p....
MicroRNAs (miRNAs) influence many biological processes, including cancer. They do so by posttranscriptionally repressing target mRNAs to which they have sequence complementarity. Although it has been postulated that miRNAs can regulate other miRNAs, this never shown experimentally our knowledge. Here, we demonstrate miR-107 negatively regulates the tumor suppressor miRNA let-7 via a direct interaction. was found be highly expressed in malignant tissue from patients with advanced breast...