A5053256947- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related Molecular Pathways
- Kruppel-like factors research
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Mechanisms of cancer metastasis
- TGF-β signaling in diseases
- Signaling Pathways in Disease
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Cell death mechanisms and regulation
- Biochemical and Molecular Research
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Retinoids in leukemia and cellular processes
- Cellular Mechanics and Interactions
- DNA Repair Mechanisms
- Cancer, Hypoxia, and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- RNA modifications and cancer
- Melanoma and MAPK Pathways
- NF-κB Signaling Pathways
Institute of Biological Chemistry, Academia Sinica
2015-2024
Academia Sinica
2011-2024
National Taiwan University
2012-2023
Institute of Molecular Medicine
2002-2023
Institute of Biomedical Sciences, Academia Sinica
2012-2022
Shenzhen Blood Center
2018
Beth Israel Deaconess Medical Center
2013
Taipei Medical University
2013
National Yang Ming Chiao Tung University
2012
National Taiwan University Hospital
2003-2012
Transforming growth factor-beta (TGF-beta) affects cellular proliferation, differentiation, and interaction with the extracellular matrix primarily through type I II TGF-beta receptors. The receptors for activin contain putative serine-threonine kinase domains. A murine receptor, Tsk 7L, was cloned that shared a conserved domain receptor. Overexpression of 7L alone did not increase cell surface binding TGF-beta, but coexpression receptor caused to bind which had size inhibited in dominant...
Transforming growth factor-β (TGF-β) is a multifunctional protein that regulates cell proliferation and differentiation extracellular matrix production. Although two receptor types, the type I II receptors, have been implicated in TGF-β-induced signaling, it unclear how many activities of TGF-β are mediated through these receptors. With use cells overexpressing truncated receptors as dominant negative mutants to selectively block existence pathways was shown. The possibly conjunction with...
Abstract Metastasis is the major cause of poor prognosis in colorectal cancer (CRC), and increasing evidence supports contribution miRNAs to progression. Here, we found that high expression miR-103 miR-107 (miR-103/107) was associated with metastasis potential CRC cell lines patients CRC. We showed miR-103/107 targeted known suppressors death-associated protein kinase (DAPK) Krüppel-like factor 4 (KLF4) cells, resulting increased motility cell–matrix adhesion decreased cell–cell epithelial...
Activation of tumor suppressors for the treatment human cancer has been a long sought, yet elusive, strategy. PTEN is critical suppressive phosphatase that active in its dimer configuration at plasma membrane. Polyubiquitination by ubiquitin E3 ligase WWP1 (WW domain-containing 1) suppressed dimerization, membrane recruitment, and function PTEN. Either genetic ablation or pharmacological inhibition triggered reactivation unleashed activity. appears to be direct MYC (MYC proto-oncogene)...
Transforming growth factor-β (TGF-β) and activin signal primarily through interaction with type I II receptors, which are transmembrane serine-threonine kinases. Tsk 7L is a receptor for TGF-β requires coexpression of the ligand binding. also specifically bound activin, when coexpressed IIA receptor. could associate either binding specificity was conferred by can therefore act as both TGF-β, possibly other ligands.
The multifunctional cytokine interleukin-6 (IL-6) regulates growth and differentiation of many cell types induces production acute-phase proteins in hepatocytes. Here we report that IL-6 protects hepatoma cells from apoptosis induced by transforming factor-β (TGF-β), a well known apoptotic inducer liver cells. Addition blocked TGF-β-induced activation caspase-3 while showing no effect on the induction plasminogen activator inhibitor-1 p15<sup>INK4B</sup> genes, indicating interferes with...
To study the effect of nucleotide excision repair on spectrum mutations induced in diploid human fibroblasts by UV light (wavelength, 254 nm), we synchronized repair-proficient cells and irradiated them when HPRT gene was about to be replicated (early S phase) so that there would no time for before replication, or G1 phase 6 h prior S, determined kinds location gene. As a control, also compared spectra populations xeroderma pigmentosum (XP12BE cells, which are unable excise UV-induced DNA...
Fucoidan, a polysaccharide extracted from brown seaweeds, reduces tumor cell proliferation. Fucoidan inhibits the growth of breast cancer cells such as 4T1 and MDA-MB-231 decreases their colony formation. Moreover, fucoidan metastatic lung nodules in xenograft female Balb/c mice. The molecular network transforming factor β (TGFβ) receptors (TGFRs) plays an important role regulation epithelial to mesenchymal transition (EMT) cells. Using cells, we found that effectively reverses TGFR-induced...
Abstract The ubiquitin–proteasome system (UPS) and autophagy are two major quality control processes whose impairment is linked to a wide variety of diseases. coordination between UPS remains incompletely understood. Here, we show that ubiquitin ligase UBE3C deubiquitinating enzyme TRABID reciprocally regulate K29/K48-branched ubiquitination VPS34. We find this enhances the binding VPS34 proteasomes for degradation, thereby suppressing autophagosome formation maturation. Under ER proteotoxic...
Extracellular vesicles (EVs) are released by cells to mediate intercellular communication under pathological and physiological conditions. While small EVs (sEVs; <100-200 nm, exosomes) intensely investigated, the properties functions of medium large (big (bEVs); >200 microvesicles) less well explored. Here, bEVs sEVs identified as distinct EV populations, it is determined that in a greater bEV:sEV ratio aggressive human triple-negative breast cancer (TNBC) subtype. PalmGRET,...
Brk (for breast tumor kinase) is a nonreceptor tyrosine kinase containing SH3, SH2, and catalytic domains. was originally identified from human metastatic tumor, its overexpression frequently observed in cancer several other types. However, the molecular mechanism by which this participates tumorigenesis remains poorly characterized. In present study, we not only paxillin as binding partner substrate of but also discovered novel signaling pathway mediates epidermal growth factor...
Pentoxifylline (PTX) is a potent inhibitor of connective tissue growth factor (CTGF), but its underlying mechanism poorly understood. Here, it was demonstrated that PTX inhibited not only TGF-beta1-induced CTGF expression also CTGF-induced collagen I (alpha1) [Col (alpha1)] in normal rat kidney fibroblasts (NRK-49F) and alpha-smooth muscle actin proximal tubular epithelial cells (NRK-52E). Furthermore, attenuated tubulointerstitial fibrosis, myofibroblasts accumulation, Col unilateral...