Sargassum hemiphyllum , a kind of brown seaweed generally found along coastlines in East Asia, has long served as traditional Chinese medicine. S. shown an anti-inflammatory effect; however, its mechanism not been elucidated clearly. This study explored for biomedical effects. sulfated polysaccharide extract (SHSP) was first prepared; the mouse macrophage cell line (RAW 264.7) activated by lipopolysaccharide (LPS) used model system. The secretion profiles pro-inflammatory cytokines,...

Activation of hypoxia-induced hypoxia-inducible factors-1 (HIF-1) plays a critical role in promoting tumor angiogenesis, growth and metastasis. Low molecular weight fucoidan (LMWF) is prepared from brown algae, exhibits anticancer activity. However, whether LMWF attenuates angiogenesis bladder cancer cells the mechanisms involved remain unclear. This first study to demonstrate that can inhibit hypoxia-stimulated H2O2 formation, HIF-1 accumulation transcriptional activity vascular endothelial...

Fucoidan, a polysaccharide extracted from brown seaweeds, reduces tumor cell proliferation. Fucoidan inhibits the growth of breast cancer cells such as 4T1 and MDA-MB-231 decreases their colony formation. Moreover, fucoidan metastatic lung nodules in xenograft female Balb/c mice. The molecular network transforming factor β (TGFβ) receptors (TGFRs) plays an important role regulation epithelial to mesenchymal transition (EMT) cells. Using cells, we found that effectively reverses TGFR-induced...

The aim of this study is to investigate the anti-inflammatory effects low-molecular-weight fucoidan (LMF) and high-stability fucoxanthin (HS-Fucox) in a lipopolysaccharide-induced inflammatory Caco-2 cell line co-culture with B. lactis.We used various methods such as transepithelial resistance (TER) assay, cytokine secretion tight junction protein mRNA expression assay examine LMF HS-Fucox properties.LMF activated probiotic growth reduced inflammation intestinal epithelial cells. Moreover,...

Accumulating evidence has revealed that fucoidan exhibits anti-tumor activities by arresting cell cycle and inducing apoptosis in many types of cancer cells including hepatocellular carcinoma (HCC). Exploring its effect on microRNA expression, we found markedly upregulated miR-29b human HCC cells. The induction was accompanied with suppression downstream target DNMT3B a dose-dependent manner. reduction luciferase activity 3′-UTR reporter as mimic, indicating induced to suppress DNMT3B....

The combined effects of low-molecular-weight fucoidan (LMF) and fucoxanthin (Fx) in terms antihyperglycemic, antihyperlipidemic, hepatoprotective activities were investigated a mouse model type II diabetes. intake LMF, Fx, LMF + Fx lowered the blood sugar fasting levels, increased serum adiponectin levels. significant decrease urinary was only observed supplementation. had ameliorating on hepatic tissue db/db mice by increasing glycogen antioxidative enzymes, more effective than at improving...

// Hsien-Yeh Hsu 1,2,3,* , Tung-Yi Lin 1,3,* Yu-Chung Wu 4,5 Shu-Ming Tsao 1 Pai-An Hwang 6 Yu-Wei Shih and Jason 7 Department of Biotechnology Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan 2 The Genomics Research Center, Academia Sinica, 3 Program Molecular University 4 Division Thoracic Surgery, Taipei Veterans General Hospital, 5 School Seafood Technology Division, Fisheries Institute, Council Agriculture, Keelung, Fordham New York, NY, USA * These authors...

Seaweed polysaccharides have been recommended as anticancer supplements and for boosting human health; however, their benefits in the treatment of triple-negative breast cancers (TNBCs) improving immune surveillance remain unclear. Olaparib is a first-in-class poly (ADP-ribose) polymerase inhibitor. Oligo-Fucoidan, low-molecular-weight sulfated polysaccharide purified from brown seaweed (Laminaria japonica), exhibits significant bioactivities that may aid disease management.Macrophage...

For a long time, fucoidan has been well known for its pharmacological activities, and recently low molecular weight (LMF) used in food supplements pharmaceutical products. In the present study, LMF was extracted from Laminaria japonica by enzyme hydrolysis. The toxicity of mouse rat models determined many methods, such as total arsenic content, bacterial reverse mutation assay, chromosome aberration vivo micronucleus assay. findings showed that at 5000 μg/mL exhibited no mutagenicity. It...

This study investigated whether the incorporation of L. japonica extract into bacterial cellulose (BC) membranes enhanced their suitability for biomedical applications such as wound dressings. was added at concentrations 10 % and 25 to BC cultures, we then analyzed its effects on BC-10 BC-25 production, chemical composition, hydration, bioactivity. We found that while did not significantly increase yield, it modified membrane microstructure by increasing water absorption reducing syneresis,...

The hot-water extract of seaweed Sargassum hemiphyllum was evaluated antioxidant activity by using four different methods, including DPPH free radicals scavenging activity, Fe+2 chelating superoxide anion radical and reducing power, the immune-stimulating HB4C5 cells J774.1 cells. It found that increasing in correlating with concentration below 3.5 mg/ml. showed maximum relative activities cell proliferation (174%) IgM secretion (132%) at 120 µg/ml extract. Overheating slightly reduced At 80...

Sargassum hemiphyllum is a common plant found on the coasts of Taiwan; it has been used as an anti-inflammatory agent in traditional herbal medicine. This study aimed to evaluate effects S. sulfated polysaccharide (SHSP) using two different mouse models. In both arachidonic acid-induced ear inflammatory gavage and paint models, SHSP decreased swelling erythema. addition, production myeloperoxidase, nitric oxide, interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α dose-dependent manner....

Low-molecular-weight Fucoidan (Oligo-Fucoidan) is a sulfated polysaccharide that has variety of biological effects and also been shown to have beneficial health effects. However, the molecular mechanisms underlying therapeutic Oligo-Fucoidan in patients with cancer remain unclear. Using human colorectal HCT116 cells (p53+/+) or without (p53-/-) normal p53 expression, we found treatment reduces occurrence spontaneous DNA lesions. Etoposide induces double strand breaks. Subsequent...

Hydroxyapatite (HAp) is the main inorganic component and an essential part of hard bone teeth. Due to its excellent biocompatibility, bioactivity, osteoconductivity, synthetic HAp has been widely used as a substitute, cell carrier, therapeutic gene or drug carrier. Recently, numerous studies have demonstrated that strontium-substituted hydroxyapatite (SrHAp) not only enhances osteogenesis but also inhibits adipogenesis in mesenchymal stem cells. Mesoporous SrHAp successfully synthesized via...

Scope Several beneficial biological functions of fucoidan (FO) isolated from brown algae have been demonstrated. The purpose this study was to investigate whether FO derived Sargassum hemiphyllum ameliorates pancreatic β‐cell damage and impaired insulin synthesis under diabetic condition. Methods results effects were studied in streptozotocin (STZ)‐treated line, NIT‐1cells, mice. cell apoptosis, protein analyses, histological examination, function assays performed. increased apoptosis...

Fucoidan, an anionic, sulfated polysaccharide from brown seaweed, is known to exhibit antitumor and immunomodulatory functions. To develop immune protection chemotherapeutic agent, fucoidan-cisplatin nanoparticles (FCNPs) were designed. FCNPs prepared by mixing cisplatin with fucoidan solution or solution, followed dialysis remove trace elements. The nanoparticles, comprising 10 mg of 2 cisplatin, which exhibited the highest content loading efficiency during production process, named as...

Abstract Tubulointerstitial fibrosis is recognized as a key determinant of progressive chronic kidney disease (CKD). Fucoidan, sulphated polysaccharide extracted from brown seaweed, exerts beneficial effects in some nephropathy models. The present study evaluated the inhibitory effect oligo-fucoidan (800 Da) on renal tubulointerstitial fibrosis. We established mouse CKD model by right nephrectomy with transient ischemic injury to left kidney. Six weeks after surgery, we fed mice at 10, 20,...