Drug resistance is an obstacle to the treatment of ovarian cancer. Using a unique cell model, we have proven previously that subpopulation cancer cells more resistant cisplatin than are original cells. MicroRNAs (miRNAs), small noncoding RNAs, involved in many biological events In our study, explored whether miRNAs Cisplatin-resistant expressed lower level miR-29a/b/c. Manipulation microRNA-29 (miR-29) expression modulated sensitivity CP70, HeyC2, SKOV3 and A2780 Knockdown miR-29a/b/c...

Accumulating evidence has revealed that fucoidan exhibits anti-tumor activities by arresting cell cycle and inducing apoptosis in many types of cancer cells including hepatocellular carcinoma (HCC). Exploring its effect on microRNA expression, we found markedly upregulated miR-29b human HCC cells. The induction was accompanied with suppression downstream target DNMT3B a dose-dependent manner. reduction luciferase activity 3′-UTR reporter as mimic, indicating induced to suppress DNMT3B....

Oncogenic activation of the Wnt/β-catenin signaling pathway is common in hepatocellular carcinoma (HCC). Our recent studies have demonstrated that SRY (sex determining region Y)-box 1 (SOX1) and secreted frizzled-related proteins are concomitantly promoter-hypermethylated, this might lead to abnormal Wnt HCC. SOX1 encodes a transcription factor involved regulation embryonic development cell fate determination. However, expression functional role HCC remains unclear. In study, we confirmed...

In this study, we observed that brown seaweed fucoidan inhibited human breast cancer progression by upregulating microRNA (miR)-29c and downregulating miR-17-5p, thereby suppressing their target genes, a disintegrin metalloproteinase 12 (ADAM12) phosphatase tensin homolog (PTEN), respectively.Moreover, reduced the luciferase activity of 3'-untranslated region reporter; treatment cells with miR-29c mimic or miR-17-5p inhibitor also produced similar results.These effects epithelial-mesenchymal...

Abstract Oncogenic activation of the Wnt/β‐catenin signaling pathway is common in hepatocellular carcinoma (HCC). The secreted frizzled‐related proteins (SFRPs) function as negative regulators Wnt and have important implications for carcinogenesis. Promoter hypermethylation SFRP genes human cancers. However, role SFRPs HCC not clear. Recently, we shown that SFRP1 frequently downregulated through promoter hypermethylation. To confirm extend these findings, methylation status other members,...

Honokiol, a hydroxylated biphenyl compound isolated from the Chinese herb Magnolia officinalis, has been reported to have anticancer activities in variety of cancer cell lines. The present study aimed evaluate effect and possible molecular mechanisms honokiol glioblastoma multiforme (GBM) line. were investigated DBTRG‑05MG GBM on growth was determined using sulforhodamine B assay. Flow cytometry immunoblotting used measure honokiol‑induced apoptosis (programmed death type I) autophagy II)....

For a long time, fucoidan has been well known for its pharmacological activities, and recently low molecular weight (LMF) used in food supplements pharmaceutical products. In the present study, LMF was extracted from Laminaria japonica by enzyme hydrolysis. The toxicity of mouse rat models determined many methods, such as total arsenic content, bacterial reverse mutation assay, chromosome aberration vivo micronucleus assay. findings showed that at 5000 μg/mL exhibited no mutagenicity. It...

Abstract Carbonic anhydrase III (CAIII) is distinguished from the other members of CA family by low carbon dioxide hydratase activity, resistance to inhibitor acetazolamide, and a predominant expression in liver males. In this report effects CAIII on cancer cells invasiveness were explored. Overexpression HCC cell line SK‐Hep1 resulted increased anchorage‐independent growth invasiveness. And siRNA‐mediated silencing decreased invasive ability cells. Furthermore, transfectants showed elevated...

Except for genetic mutations, epigenetic changes are also involved in the development of human cancers. Recently, we have identified SOX1, SRY (sex determining region Y)-box 1, is hypermethylated cervical cancer and ovarian cancer. Therefore, investigated whether promoter hypermethylation SOX1 common hepatocellular carcinoma (HCC).We used methylation-specific polymerase chain reaction (MS-PCR) bisulfite sequencing to analyze methyaltion level seven HCC cell lines, 54 clinical HCCs, 42...

Mefloquine (MQ) is a prophylactic anti-malarial drug. Previous studies have shown that MQ induces oxidative stress in vitro. Evidence indicates reactive oxygen species (ROS) may be used as therapeutic modality to kill cancer cells. This study investigated whether also inhibits prostate (PCa) cell growth. We sulforhodamine B (SRB) staining determine viability. has highly selective cytotoxicity PCa The antitumor effect was most significant when examined using colony formation assay....

Most endometrial carcinomas appear to develop from precursors (e.g., hyperplasia) that progress for several years. Patients who are ultimately diagnosed with carcinoma often present clinically complaints of abnormal vaginal bleeding years before diagnosis, which offers an opportunity early diagnosis and curative treatment. The analysis DNA methylation may be used as a method detecting cancer (EC). To test the potential clinical application this method, we quantitative five genes in full...

Mefloquine (MQ) is currently in clinical use as a prophylactic treatment for malaria. Previous studies have shown that MQ induces oxidative stress vitro. The present study investigated the anticancer effects of PC3 cells. cell viability was evaluated using sulphorhodamine-B (SRB) staining, while annexin V and propidium iodide (PI) were used an assay death. Reactive oxygen species (ROS) formation detected with 2',7'‑dichlorofluorescein‑diacetate (DCFH‑DA), sensitive intracellular probe,...

KG-135, a standardized formulation enriched with Rk1, Rg3, and Rg5 ginsenosides, has been shown to inhibit various types of cancer cells; however, the underlying mechanisms are not fully understood. In this study, we explored its effects in A549 human lung cells investigate induction Forkhead Class box O3a (FOXO3a) autophagy.Cell viability was determined by sulforhodamine B staining. Apoptosis cell cycle distribution were analyzed using flow cytometry. The changes protein levels Western blot...

Oncogenic activation of the Wnt/ β -catenin signaling pathway is common in human cancers. The secreted frizzled-related proteins (SFRPs) function as negative regulators Wnt and have important implications carcinogenesis. Because there been no reports about role SFRP3 hepatocellular carcinoma (HCC), we investigated level methylation transcription . Four HCC cell lines, 60 HCCs, 23 cirrhosis livers, 37 chronic hepatitis 30 control livers were prescreened for promoter by methylation-specific...

Activation of human MAGE genes leads to the expression a set tumor rejection antigens, which are recognized by cytotoxic T lymphocytes. The antigens may become targets immunotherapy. was originally found in, but is not restricted, melanomas. aim this study investigate in hepatocellular carcinomas.The MAGE-1, -2, -3, -4 tumorous and corresponding non-tumorous liver tissue studied using reverse-transcription polymerase chain reaction.In 50 carcinomas studied, mRNA detected 23 (46%), 17 (34%),...

Related to genetic alteration, frequent promoter hypermethylation is also a contributing factor in the development of human cancers. Recently, we discovered numerous novel genes that were aberrantly methylated hepatocellular carcinoma (HCC) by using Infinium HumanMethylation27 BeadChip array. We utilized quantitative methylation-specific PCR (Q-MSP) system for evaluation PAX6 methylation 29 normal controls and 160 paired HCC tissues their adjacent non-tumor tissues. verified correlation...