Electrospinning is a simple and efficient method of fabricating non-woven polymeric nanofiber matrix. However, using fluorinated alcohols as solvent for the electrospinning proteins often results in protein denaturation. TEM circular dichroism analysis indicated massive loss triple-helical collagen from an electrospun (EC) matrix, random coils were similar to those found gelatin. Nevertheless, mechanical testing we Young's modulus ultimate tensile stresses EC matrices significantly higher...

Gold nanorods emit strong photoluminescence under two photon excitation; the efficient lumininescence (TPL) arises from local field enhancement assisted by surface plasmons. The plasmon effects on TPL efficiency and spectrum are investigated measuring of gold with various aspect ratios. A large is found when incident light wavelength coincides longitudinal mode nanorods. However, emission spectra ratios look similar exhibit modest features, which implies a major non-radiative decay excited

This study investigated whether the incorporation of L. japonica extract into bacterial cellulose (BC) membranes enhanced their suitability for biomedical applications such as wound dressings. was added at concentrations 10 % and 25 to BC cultures, we then analyzed its effects on BC-10 BC-25 production, chemical composition, hydration, bioactivity. We found that while did not significantly increase yield, it modified membrane microstructure by increasing water absorption reducing syneresis,...

Hydroxyapatite (HAp) is the main inorganic component and an essential part of hard bone teeth. Due to its excellent biocompatibility, bioactivity, osteoconductivity, synthetic HAp has been widely used as a substitute, cell carrier, therapeutic gene or drug carrier. Recently, numerous studies have demonstrated that strontium-substituted hydroxyapatite (SrHAp) not only enhances osteogenesis but also inhibits adipogenesis in mesenchymal stem cells. Mesoporous SrHAp successfully synthesized via...

Abstract Collagen, a critical part of the extra‐cellular matrix tissues, is popular native material for building scaffolding tissue‐engineering applications. To mimic structural and functional profiles materials found in matrix, numerous efforts have been made toward developing novel scaffold combining collagen with other biomacromolecules. All these works focused on improving mechanical or biochemical properties collagen‐based matrix. Unfortunately, most studies failed to consider...

Abstract: Hyaluronan–cisplatin conjugate nanoparticles (HCNPs) were chosen as colon-targeting drug-delivery carriers due to the observation that a variety of malignant tumors overexpress hyaluronan receptors. HCNPs prepared by mixing cisplatin with solution, followed dialysis remove trace elements. The cells treated showed significantly lower viability than those alone. entrapped in Eudragit S100-coated pectinate/alginate microbeads (PAMs) using an electrospray method and polyelectrolyte...

AbstractTetracyclines (TCs), including tetracycline (TC), oxytetracycline (OTC), and chlortetracycline (CTC), are amongst the most common antibiotics used in animal husbandry. Residual amounts of these environment a concern because they contribute to selection resistant bacteria. In this study, we investigated biodegradation three TCs swine wastewater. batch experiments, OTC CTC were completely degraded at d 18 20, respectively, but TC was remained 7.1% after 20 incubation. The degradation...

Fucoidan, an anionic, sulfated polysaccharide from brown seaweed, is known to exhibit antitumor and immunomodulatory functions. To develop immune protection chemotherapeutic agent, fucoidan-cisplatin nanoparticles (FCNPs) were designed. FCNPs prepared by mixing cisplatin with fucoidan solution or solution, followed dialysis remove trace elements. The nanoparticles, comprising 10 mg of 2 cisplatin, which exhibited the highest content loading efficiency during production process, named as...

Mesoporous bioactive glass nanofibers (MBGNFs) were prepared by a sol–gel/electrospinning technique. Subsequently, collagen–MBGNF (CM) composite scaffold that simultaneously possessed macroporous structure and collagen was fabricated gelation freeze-drying process. Additionally, immersing the CM in simulated body fluid resulted formation of bone-like apatite minerals on surface. The provided suitable environment for attachment to cytoskeleton. Based measured alkaline phosphatase activity...

Poly(ε-caprolactone) (PCL) membranes have been widely used in guided tissue regeneration (GTR) and bone (GBR). In addition, hydroxyapatite is the major inorganic component an essential composition of hard teeth. Recently, numerous studies demonstrated that strontium-substituted (SrHA) not only enhances osteogenesis but also inhibits adipogenesis mesenchymal stem cells. Therefore, SrHA incorporated into PCL could be alternative material for GBR. this study, nanofibers (SrHANFs) were...

In this study, we demonstrate that the uptake rate of surface-modified gold nanoparticles (GNPs) with folic acid by specific cells can be increased significantly, if membranes these have sufficient folic-acid receptors. Two human breast cancer cell lines were studied; one is MDA-MB-435S cell, and other T-47D cell. The expression receptors former much higher than latter. These incubated media containing bare GNPs or conjugated individually. Due to unique optical behavior (i.e. surface plasmon...

Natural bone tissue consists primarily of bioapatite and collagen. Synthetic hydroxyapatite (HA) possesses good biocompatibility, bioactivity, osteoconductivity due to its chemical biological similarity bioapatite. Hence, HA has been widely used as a graft, cell carrier drug/gene delivery carrier. Moreover, strontium-substituted (SrHA) can enhance osteogenic differentiation inhibit adipogenic mesenchymal stem cells. SrHA the potential be graft for regeneration. It is accepted that adhesion...

Abstract: The purpose of the current study is to evaluate carrier capability collagen‐hydroxyapatite microspheres bone morphogenic proteins (BMP). After anesthesia, a defect (6.0 mm in diameter and 10.0 depth) was created at distal femoral condyles New Zealand white rabbits. These 3 defects were then completely filled with implant materials. 2, 4, 6, 8 weeks, animals sacrificed histological evaluations performed. results showed that when left untreated, there no evidence formation during...

Platelet rich plasma (PRP), which includes many growth factors, can activate osteoid production, collagen synthesis and cell proliferation. Nanohydroxyapatite-type I beads (CIB), mimetic natural bone components, are not only flexible fillers for defect but also encourage osteogenesis. Bone marrow mesenchymal stem cells (BMSCs) often used as an abundant source tissue engineering. We a rabbit model to combine PRP, CIB BMSCs (CIB+PRP+BMSC) into bone-like substitute study its impact on...

Hydroxyapatite (HAp), a major inorganic and essential component of normal bone teeth, is promising biomaterial due to its excellent biocompatibility, bioactivity, osteoconductivity. Therefore, synthetic HAp has been widely used as substitute, cell carrier, delivery carrier therapeutic genes or drugs. Mesoporous materials have attracted considerable attention their relatively high surface area, large pore volume, porosity, tunable size. Recently, mesoporous also successfully synthesized by...

Abstract Our objectives were to fabricate three‐dimensional collagen/chondroitin sulfate beads using mild conditions and no chemical reagents, subsequently investigate the influence of nanotopography these on primary osteoblast MG63 cell responses, including morphology, proliferation rate, gene expression. The major principle used prepare was complex coacervation, with which we could obtain a collagen matrix or without characteristic D‐pattern. Therefore, utilized osteoblasts MG63, an...

Schwann cells play a critical role in the repair of peripheral nerve. The goal this study was to fabricate electrospun gelatin (Gel) and hyaluronan‐gelatin (HA‐Gel) composite nanofibers provide suitable growth environment for cells. fiber diameters Gel, 0.5 HA‐Gel, 1 1.5 HA‐Gel were 130 ± 30 nm, 294 87 362 129 224 54 respectively. biological performance Gel evaluated using an vitro culture RT4‐D6P2T rat We found that cell attachment proliferation rates not significantly different on these...