Abstract: Metal pins used to apply skeletal traction or external fixation devices protruding through skin are susceptible the increased incidence of pin site infection. In this work, we tried establish photokilling effects titanium dioxide (TiO 2 ) nanoparticles on an orthopedic implant with in vitro study. these photocatalytic experiments, aqueous TiO was added tested microorganism. The time effect photoactivation evaluated, and loss viability five different bacteria suspensions (...

In osteoarthritis (OA), the imbalance of chondrocytes' anabolic and catabolic factors can induce cartilage destruction. Interleukin-1 beta (IL-1β) is a potent pro-inflammatory cytokine that capable inducing chondrocytes synovial cells to synthesize MMPs. The hypoxia-inducible factor-2alpha (HIF-2alpha, encoded by Epas1) transcription factor in osteoarthritic process. purpose this study validate effects ecdysteroids (Ecd) on IL-1β-induced catabolism possible role Ecd treatment or prevention...

In this work, a thermo-induced shape-memory drug-eluting stent (SMDES) has been developed by cross-linking PEG-PCL copolymer (cPEG-PCL). The is able to perform the effect from temporary linear form permanent spiral shape with transition temperature close body temperature. incorporates controlled dual drug-release system for purpose of preventing in-stent restenosis vessel short- and long-term therapeutic effects. From results, (1)H NMR GPC indicate that compositions block copolymers are...

The purpose of this study is to develop an injectable thermoresponsive hydrogel system that can undergo sol–gel phase transition by the stimulation body temperature with improved mechanical stability and biocompatibility as a controlled drug delivery carrier for cancer therapy. Hexamethylene diisocyanate (HDI) was introduced into Pluronic F127 chain extender improve stability. HDI–Pluronic copolymer then incorporated hyaluronic acid nanocomposite system. physiochemical properties were...

Peripheral nerve injuries, causing sensory and motor impairment, affect a great number of patients annually. It is therefore important to incorporate different strategies promote healing. Among the treatment options, however, efficacy conduits often compromised by their lack living cells, insufficient growth factors, absence extracellular matrix (ECM)-like structure. To improve functional recovery, we aimed develop natural biodegradable multichanneled scaffold characterized with aligned...

Age-related orthopedic disorders and bone defects have become a critical public health issue, cell-based therapy is potentially novel solution for issues surrounding tissue engineering regenerative medicine.Long-term cultures of primary cells exhibit phenotypic functional degeneration; therefore, culturing or tissues suitable clinical use remain challenge.A platform consisting human osteoblasts (hOBs), calcium-alginate (Ca-Alginate) scaffolds, self-made bioreactor system was established...

Abstract Electric stimulation has been used successfully to treat a wide range of bone disorders. However, the mechanism by which electric fields can influence cells behavior remains poorly understood. The purpose this research was assess possible stimulatory effect pulsed electromagnetic field (PEMF) on cells. A PEMF with frequency 15 Hz (1 G [0.1 mT]; strength 2 mV/cm) were applied neonatal mouse calvarial cell cultures for 14 days. temporal effects osteoblasts evaluated status...

In the present study, magnetic HAP was synthesized at different ratios of Fe:Ca (XFe/Ca) by co-precipitation method. We have evaluated essential properties including crystal structure and cell parameters XRD, lattice arrangement HR-TEM, composition analysis ICP-MS, functional groups FTIR. The morphology magnetization were investigated SEM AFM SQUID, respectively. in vitro biocompatibility also with a lactate dehydrogenase assay. results showed that molecular magnetic-HAP nanoparticle...

The development of a novel alloplastic graft with both osteoinductive and osteoconductive properties is still necessary. In this study, we tried to synthesize biomimetic hydroxyapatite microspheres (gelatin/nano-hydroxyapatite microsphere embedded stromal cell-derived factor-1: GHM-S) from nanocrystalline hydroxyapatites investigate their therapeutic potential effects on bone regeneration. was synthesized by co-precipitation calcium hydroxide orthophosphoric acid gelatin solution. microbial...

Mechanical perturbations serve as extracellular signals to a variety of cells, including bone cells. Low-intensity pulsed ultrasound produces significant multifunctional effects that are directly relevant formation and resorption. Ultrasound stimulation has been shown accelerate bone-defect healing trabecular regeneration. In this study, we use an in vitro cell culture model investigate the effect low-intensity ultrasound. The rat alveolar mononuclear cell–calvaria osteoblast coculture...

With advances in ceramics technology, calcium phosphate bioceramics have been applied as bone substitutes for several decades. The focus of this work is to elucidate the biocompatibility particulates various cytotoxicities. Four different kinds powders, including beta-tricalcium (beta-TCP), hydroxyapatite (HA), beta-dicalcium pyrophosphate (beta-DCP), and sintered (SDCP), were tested by osteoblast cell culture. results analyzed count, concentration transforming growth factor-beta 1 (TGF-beta...

Abstract Background Caffeine consumption has been reported to decrease bone mineral density (BMD), increase the risk of hip fracture, and negatively influence calcium retention. In this study, we investigated caffeine on osteoblasts behaviour. Method Osteoblasts derived from newborn Wistar-rat calvaria was used in study. The effects various concentrations cell activities were evaluated by using MTT assay. Alkaline phosphatase (ALP) staining, von Kossa staining biochemical parameters...