A5050661463- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Dental materials and restorations
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Nanoplatforms for cancer theranostics
- Dental Implant Techniques and Outcomes
- Cellular Mechanics and Interactions
- Laser-Ablation Synthesis of Nanoparticles
- Lipid Membrane Structure and Behavior
- Collagen: Extraction and Characterization
- Advanced Nanomaterials in Catalysis
- Laser Applications in Dentistry and Medicine
- Nanoparticle-Based Drug Delivery
- Silk-based biomaterials and applications
Chang Gung University
2013-2018
Linkou Chang Gung Memorial Hospital
2016
National Taiwan Ocean University
2013
The long-term toxicity effects of gold nanoparticles (GNPs) on the proliferation and differentiation a progenitor cell line, MG63 osteoblast-like cells, was investigated. These cells were treated for 20 hours with two media that contained 10 nm GNPs at concentrations 1 ppm ppm. mitosis GNP-treated observed after least 21 using dark-field fluorescence microscopy. TEM, LSCM hyperspectral images indicated late endosomes in aggregated caused by vesicle fusion. Subsequently, days being cultured...
The specific properties of gold nanoparticles (AuNPs) make them a novel class photothermal agents that can induce cancer cell damage and even death through the conversion optical energy to thermal energy. Most relevant studies have focused on increasing precision targeting, improving efficacy transfer, exploring additional functions. Nevertheless, most cells uptake nanosized particles nonspecific endocytosis; therefore, before hyperthermia via AuNPs be applied for clinical use, it is...
Schwann cells play a critical role in the repair of peripheral nerve. The goal this study was to fabricate electrospun gelatin (Gel) and hyaluronan‐gelatin (HA‐Gel) composite nanofibers provide suitable growth environment for cells. fiber diameters Gel, 0.5 HA‐Gel, 1 1.5 HA‐Gel were 130 ± 30 nm, 294 87 362 129 224 54 respectively. biological performance Gel evaluated using an vitro culture RT4‐D6P2T rat We found that cell attachment proliferation rates not significantly different on these...
A highly ordered, mesoporous (pore size 2~50 nm) bioactive glass (MBG) structure has a greater surface area and pore volume excellent bone-forming bioactivity compared with traditional glasses (BGs). Hence, MBGs have been used in drug delivery bone tissue engineering. can be developed as either dense or porous block. Compared block, microbeads provide flexibility for filling different-shaped cavities are suitable culturing cells vitro. In contrast, the fibrous of scaffold shown to increase...
Herein, we describe an approach that immobilizes low-molecular-weight hyaluronic acid (low-MW HA) on the surface of gold nanoparticles (GNPs), which can serve as a cellular probe and photodamage media, to evaluate selectivity efficiency HA-based GNPs (HGNPs) mediator laser-induced photothermal cell damage. In addition, it is known solid tumors contain higher content low-MW HA than normal tissues. Thus, used rather high-MW in other studies. present study, conjugated HA, linear polysaccharide...
In this study, we utilized a mandrel rotating collector consisting of two parallel, electrically conductive pieces tape to fabricate aligned electrospun polycaprolactone/gelatin (PG) and carbon nanotube/polycaprolactone/gelatin (PGC) nanofibrous matrices. Furthermore, examined the biological performance PGC film matrices using an in vitro culture RT4‐D6P2T rat Schwann cells. Using cell adhesion tests, found that nanotube inhibited attachment on However, proliferation rates cells were higher...
Numerous methods have been developed for preparing guiding channels/tracks to promote the alignment of highly oriented cell types. However, these manufacture cannot fabricate interconnected channels within three-dimensional (3D) scaffolds. Providing a suitable architectural scaffold attachment could lead cells more rapidly display desired phenotype and perform their unique functions. Previously, we simple device composed pneumatic membrane that can generate tunable vibration frequency apply...
There are many techniques for preparing two-dimensional aligned fibril matrices. However, the critical problem associated with these is destruction of native structure (e.g., α-helix) proteins. Moreover, most cannot create a three-dimensional (3D), reconstituted collagen matrix in one step. In this study, we used simple device composed pneumatic membrane that generates tunable vibration frequency to apply physical stimulation fabricate 3D, characteristic D-period Using second harmonic...