A5102911534- Computational Drug Discovery Methods
- Cognitive Abilities and Testing
- Influenza Virus Research Studies
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory mediators and NSAID effects
- Receptor Mechanisms and Signaling
- Phosphodiesterase function and regulation
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- RNA and protein synthesis mechanisms
- Melanoma and MAPK Pathways
- Protein Kinase Regulation and GTPase Signaling
- Cancer therapeutics and mechanisms
- Genetics and Neurodevelopmental Disorders
- Parkinson's Disease Mechanisms and Treatments
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- HER2/EGFR in Cancer Research
- Hippo pathway signaling and YAP/TAZ
- Rough Sets and Fuzzy Logic
- Phytochemicals and Antioxidant Activities
Chang Gung University
2009-2024
Taipei Medical University
2013-2021
Academia Sinica
2019-2020
National Tsing Hua University
2020
National Taiwan University
2004-2018
National Taiwan Normal University
2010-2018
Chang Gung Memorial Hospital
2016-2017
Linkou Chang Gung Memorial Hospital
2017
National Cheng Kung University
2013-2016
National Yang Ming Chiao Tung University
2016
Abstract Metastasis is the major cause of poor prognosis in colorectal cancer (CRC), and increasing evidence supports contribution miRNAs to progression. Here, we found that high expression miR-103 miR-107 (miR-103/107) was associated with metastasis potential CRC cell lines patients CRC. We showed miR-103/107 targeted known suppressors death-associated protein kinase (DAPK) Krüppel-like factor 4 (KLF4) cells, resulting increased motility cell–matrix adhesion decreased cell–cell epithelial...
Abstract Fructose (Fru) is a major storage form of sugars found in vacuoles, yet the molecular regulation vacuolar Fru transport poorly studied. Although SWEET17 (for SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS17) has been characterized as exporter leaves, its expression leaves low. Here, RNA analysis and SWEET17-β-glucuronidase/-GREEN FLUORESCENT PROTEIN fusions expressed Arabidopsis (Arabidopsis thaliana) reveal that highly cortex roots localizes to tonoplast root cells. Expression was...
Few studies have examined the association between environmental phthalate exposure and children's neurocognitive development. This longitudinal study cognitive function in relation to pre-and postnatal children 2–12 years old. We recruited 430 pregnant women their third trimester Taichung, Taiwan from 2001–2002. A total of 110, 79, 76, 73 were followed up at ages 2, 5, 8, 11, respectively. evaluated four different time points using Bayley Wechsler tests for assessing functions intelligence...
Brk (for breast tumor kinase) is a nonreceptor tyrosine kinase containing SH3, SH2, and catalytic domains. was originally identified from human metastatic tumor, its overexpression frequently observed in cancer several other types. However, the molecular mechanism by which this participates tumorigenesis remains poorly characterized. In present study, we not only paxillin as binding partner substrate of but also discovered novel signaling pathway mediates epidermal growth factor...
Overexpression of epidermal growth factor receptor (EGFR) has been associated with cancer. Targeted inhibition the EGFR pathway shown to limit proliferation cancerous cells. Hence, we employed Traditional Chinese Medicine Database (TCM Database@Taiwan) (http://tcm.cmu.edu.tw) identify potential inhibitor. Multiple Linear Regression (MLR), Support Vector Machine (SVM), Comparative Molecular Field Analysis (CoMFA), and Similarities Indices (CoMSIA) models were generated using a training set...
Abstract Breast tumor kinase (Brk), an Src-like nonreceptor tyrosine kinase, is overexpressed in breast cancer and several other types. Our previous study indicates that Brk promotes cell migration invasion by phosphorylating the focal adhesion protein paxillin. Here, we report identification of p190RhoGAP-A (p190) as a substrate. phosphorylates p190 at Y1105 residue both vitro vivo, thereby promoting association with p120RasGAP (p120). As consequence, stimulates attenuates p120 functions,...
Abstract Human epidermal growth factor receptor 2, HER2, is a commonly over-expressed tyrosine kinase found in many types of carcinoma. Despite that there are several HER2 inhibitors, namely Iressa, Tarceva and Tykerb, currently clinical trials, all can cause side effects. In this study, both structure-based ligand-based drug design were employed to novel inhibitors from traditional Chinese medicine (TCM). The structure model was built homology modeling based on known receptors the same...
Click to increase image sizeClick decrease sizeKey words: Traditional Chinese medicine (TCM)Cloud-computingDockingScreeningSystems biology
Cancer stemness drives tumor initiation, progression, metastasis, recurrence, and therapy resistance. However, mechanisms that potentiate the acquisition maintenance of fate cancer cells remain incompletely understood. Here, we show miR-103/107 stimulate multiple stem-like features in colorectal cancer, including expression markers, appearance side-population cells, capabilities self-renewal, chemoresistance. Mechanistically, these stemness-promoting functions are mediated by...
Protein deubiquitination contributes to cortical development, and its dysregulation is linked intellectual disability.
Abstract Peroxisome proliferator-activated receptors α, δ, and γ are a collection of ligand-activated transcription factors crucial in lipid glucose homeostasis. The involvement these metabolism makes them perfect therapeutic target for treating obesity stroke. In this study, 'sum activity' model was employed to design multi-target agonists. We used new strategy agonists that fit both α δ but not avoid side effect. CoMFA CoMSIA models were explore the pharmacophore features by constructing...
Abstract The rapid spread of influenza virus subtype H1N1 poses a great threat to million lives worldwide. To search for new anti-influenza compounds, we performed molecular docking and dynamics simulation identify potential traditional Chinese medicine (TCM) constituents that could block M2 channel activity. Quinic acid, genipin, syringic cucurbitine, fagarine, methyl isoferulate all have extremely well results as compared control amantadine. Further de novo drug design suggests derivatives...
Microsomal prostaglandin E synthase-1 (mPGES-1) is the key enzyme for E2 (PGE2) generation during inflammation and a potential target designing anti-inflammatory drugs. Potential inhibitors of m-PGES-1 were selected from traditional Chinese medicine (TCM Database@Taiwan) based on pharmacophore map generated by top HypoGen hypothesis validated using structure- ligand-based analysis. Key features include pi-interactions H-bond donors. TCM compounds, shanciol B, A, castilliferol, aurantiamide...
Mutations in the gene encoding Ca2+-independent phospholipase A2 group 6 (PLA2G6) cause recessive familial type 14 of Parkinson's disease (PARK14). Mitochondrial dysfunction is involved pathogenesis (PD). PLA2G6 believed to be required for maintaining mitochondrial function. In present study, rotenone-induced cellular model PD was used investigate possible molecular pathogenic mechanism PARK14 mutant PLA2G6-induced PD. Overexpression wild-type (WT) ameliorated apoptotic death SH-SY5Y...
Aberrations in cyclic adenosine monophosphate (cAMP) signaling cascade has been linked to the allergic responses that associate with risks of stroke or cardiovascular diseases. Phosphodiesterase 4D (PDE4D) shown be highly involved cAMP regulation and is hence implied a potential drug target prevention. To identify PDE4D inhibitors from traditional Chinese medicine (TCM), we employed machine learning modeling techniques screen comprehensive TCM database. The multiple linear regression (MLR)...
Apoptosis resistance is a hallmark of cancer linked to disease progression and treatment resistance, which has led the development anticancer therapeutics that restore apoptotic function. Antiapoptotic Bcl-2 frequently overexpressed in refractory prostate increased following standard hormonal therapy chemotherapy; however, rationally designed antagonist, ABT-737, not shown single agent apoptosis-promoting activity against human cell lines. This likely due coordinate expression antiapoptotic,...
Abstract The swine influenza virus (H1N1) 2009 pandemic highlights the importance of having effective anti-viral strategies. Recently, oseltamivir (Tamiflu) resistant viruses are identified; which further emphasizes urgency in developing new antiviral agents. In replication cycle, viral surface glycoprotein, hemagglutinin, is responsible for entry into host cells. Hence, a potentially strategy to inhibit mechanism. To develop novel agent that inhibits entry, we analyzed 20,000 traditional...