A5013548922- Glycosylation and Glycoproteins Research
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Galectins and Cancer Biology
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Histone Deacetylase Inhibitors Research
- Medicinal Plants and Neuroprotection
- Influenza Virus Research Studies
- Cancer, Lipids, and Metabolism
- Cancer Research and Treatments
- Cancer-related gene regulation
- Estrogen and related hormone effects
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Essential Oils and Antimicrobial Activity
- Viral gastroenteritis research and epidemiology
- Cancer Treatment and Pharmacology
- Respiratory viral infections research
- Wnt/β-catenin signaling in development and cancer
- Immune cells in cancer
- Macrophage Migration Inhibitory Factor
- Phytochemistry and Biological Activities
- Liver physiology and pathology
- Protease and Inhibitor Mechanisms
Agricultural Biotechnology Research Center, Academia Sinica
2015-2024
National Defense Medical Center
2007-2024
National Defense Medical College
2023-2024
Biotechnology Research Center
2008-2023
Academia Sinica
2008-2023
Chung Shan Medical University
2020
National Taiwan University
2007-2017
National Chung Hsing University
2015
National Yang Ming Chiao Tung University
2013
Institute of Biological Chemistry, Academia Sinica
2013
In this study, 221 phytocompounds were evaluated for activity against anti-severe acute respiratory syndrome associated coronavirus (SARS-CoV) activities using a cell-based assay measuring SARS-CoV-induced cytopathogenic effect on Vero E6 cells. Ten diterpenoids (1-10), two sesquiterpenoids (11 and 12), triterpenoids (13 14), five lignoids (15-19), curcumin (20), reference controls niclosamide (21) valinomycin (22) potent inhibitors at concentrations between 3.3 10 microM. The of the 22...
Tumor suppressor gene silencing through cytosine methylation contributes to cancer formation. Whether DNA demethylation enzymes counteract this oncogenic effect is unknown. Here, we show that TET1, a dioxygenase involved in demethylation, downregulated prostate and breast tissues. TET1 depletion facilitates cell invasion, tumor growth, metastasis xenograft models correlates with poor survival rates patients. Consistently, enforced expression of reduces invasion Mechanistically, suppresses...
Although the linkage of polyglutamine (poly-Q) repeat expansion in androgen receptor (AR) to Kennedy's disease (X-linked spinal and bulbar muscular atrophy) was a major step forward, detailed molecular mechanism how change poly-Q length contributes remains unclear. Here we report identification nuclear G-protein, Ras-related protein/ARA24, as first AR coactivator that can bind differentially with different lengths within AR. In yeast mammalian reciprocal interacting assays, our data...
Nuclear hormone receptors are ligand-dependent transcriptional regulators that modulate chromatin structure. However, the precise molecular mechanisms by which recruit chromatin-remodeling activity not fully elucidated. We show in absence of its ligand-binding domain, glucocorticoid receptor (GR) is able to interact with both nuclear coactivators and BRG1 complex vivo. Individually, GR makes direct interactions BRG1-associated factor 60a (BAF60a) BAF57, but BRG1, BAF155, or BAF170. Further,...
Abstract The retinoblastoma binding protein RBP2 (KDM5A) is a histone demethylase that promotes gastric cancer cell growth and enriched in drug-resistant lung cells. In tumor-prone mice lacking the tumor suppressor gene RB or MEN1, genetic ablation of can suppress initiation, but pathogenic breadth mechanistic aspects this effect relative to human tumors have not been defined. Here, we approached question context cancer. was overexpressed tissues where its depletion impaired proliferation,...
Dysregulation of androgen signaling and pericellular proteolysis is necessary for prostate cancer progression, but the links between them are still obscure. In this study, we show how membrane-anchored serine protease TMPRSS2 stimulates a proteolytic cascade that mediates androgen-induced cell invasion, tumor growth, metastasis. We found matriptase serves as substrate in mediating proinvasive action androgens cancer. Further, determined higher levels expression correlate with activation...
Arginine plays diverse roles in cellular physiology. As a semi-essential amino acid, arginine deprivation has been used to target cancers with synthesis deficiency. Arginine-deprived cancer cells exhibit mitochondrial dysfunction, transcriptional reprogramming and eventual cell death. In this study, we show prostate that acts as an epigenetic regulator modulate histone acetylation, leading global upregulation of nuclear-encoded oxidative phosphorylation (OXPHOS) genes. TEAD4 is retained the...
The retinoblastoma protein may function as a tumor suppressor by controlling the progression of normal cell cycle. Inactivation Rb has been regarded an important event in prostate carcinogenesis. However, detailed mechanism how is linked to androgen-androgen receptor (A-AR), major factor promotion growth, remains unclear. Using GST-Rb pull down assay and mammalian two-hybrid system, we report here that can bind specifically AR androgen-independent manner. Transient transfection demonstrates...
Chronic inflammation can augment tumor development in various types of cancers, including prostate cancer (PCa). Reduction is therefore an important anticancer therapeutic opportunity. Here, we report four anti-proliferative phytocompounds Wedelia chinensis , oriental herbal medicine, identified through their ability to modulate the androgen receptor (AR) activation transcription from prostate-specific antigen promoter PCa cells. The 50% inhibition concentration values...
Wedelia chinensis is a common ingredient of anti-inflammatory herbal medicines in Taiwan and southern China. Inflammation involved promoting tumor growth, invasion, metastasis. This study aims to test the biological effects vivo W. extract on prostate cancer.The efficacy mechanisms action oral administration standardized were analyzed animals bearing subcutaneous or orthotopic cancer xenograft.Exposure cells induced apoptosis selectively androgen receptor (AR)-positive shifted proportion...
This study is aimed at investigating antineoplastic efficacy of histone deacetylase inhibitor (HDACI) LBH589 on renal cell carcinoma (RCC) and elucidating the novel molecular mechanisms involved in growth arrest apoptosis by targeting important nonhistone molecules.We analyzed growth-inhibitory effect RCC 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay vitro antitumor xenograft experiments vivo. To verify associated LBH589-mediated death cycle progression Western blotting...
Influenza A viruses are major human and animal pathogens with huge economic societal impact from illness, hospitalizations, deaths. Virus-like particles (VLPs) of influenza virus have been suggested as a vaccine candidate offering improved safety efficacy. To develop this concept further, we established flexible platform to efficiently generate different subtypes mammalian-expressed VLPs. Here demonstrate that these mammalian VLPs strongly resemble the authentic in structure, particle size...
The tumor cell lysate-pulsed, dendritic (DC)-based cancer vaccine approaches are being actively evaluated for application to immunotherapy, hopefully at a personalized medicine base. There is apparently an emerging technical problem however, the lack of highly efficacious potency in activation patient's DCs T-cell priming and associated process presenting immunogenicity. One strategy address this consider manipulation immunogenic cells death (ICD) complex ex-vivo maximal DC efficacy. In our...
Programmed necrosis, necroptosis, is considered to be a highly immunogenic activity, often mediated via the release of damage-associated molecular patterns (DAMPs). Interestingly, enhanced macroautophagic/autophagic activity found accompanied by necroptosis. However, possible role autophagy in immunogenicity necroptotic death remains largely obscure. In this study, we investigated mechanistic correlation between phytochemical shikonin-induced and necroptosis for tumor immunogenicity. We show...
Abstract Metastatic prostate cancer continues to pose a difficult therapeutic challenge. Prostate progression is associated with aberrant O-glycosylation of cell surface receptors, but the functional impact such events uncertain. Here we report spontaneous metastasis human xenografts that express high levels galectin-4 along genetic signatures EGFR-HER2 signaling and O-glycosylation. Galectin-4 expression in clinical specimens correlated poor patient survival. binding multiple receptor...
Transforming growth factor (TGF) β1 facilitates FSH-induced differentiation of rat ovarian granulosa cells. The signaling crosstalk between follicle stimulating hormone (FSH) and TGFβ receptors remains unclear. This study was to investigate the interplay cAMP/protein kinase A (PKA) phosphatidylinositol-3-kinase (PI3K) including mammalian target rapamycin (mTOR)C1 dependence in FSH- TGFβ1-stimulated steroidogenesis To achieve this aim, inhibitors PKA (PKAI), PI3K (wortmannin), mTORC1...
ABSTRACT Disseminated castration‐resistant prostate cancer (CRPC) is a common disease in men that characterized by limited survival and resistance to androgen‐deprivation therapy. The increase human epidermal growth factor receptor 2 (HER2) signaling contributes androgen activity subset of patients with CRPC; however, enigmatically, HER2‐targeted therapies have demonstrated lack efficacy CRPC. Aberrant glycosylation hallmark involves key processes support progression. Using transcriptomic...
Recurrence and metastasis are the main causes of triple-negative breast cancer (TNBC) mortality. On basis our clinical cohorts integrative omics analyses, we hypothesized that understanding interplay between fatty acid binding protein (FABP) epoxy-eicosatrienoic (EET) driven metastatic progression can uncover a new opportunity for TNBC intervention. In this study, biological relevance increased expression CYP2C19, FABP4, FABP5 in tumors cell line (MDA-MB-231), as well its highly lung seeking...
Two mosquito STATs, AaSTAT and CtSTAT, have been cloned from Aedes albopictus Culex tritaeniorhynchus mosquitoes, respectively. These two STATs are more similar to those of Drosophila, Anopheles, mammalian STAT5 in the DNA binding Src homology 2 domains. The mRNA transcripts expressed at all developmental stages, proteins present predominantly pupal adult stages both mosquitoes. Stimulation with lipopolysaccharide resulted an increase tyrosine phosphorylation activity CtSTAT as well...
Curcumin has been shown to possess potent chemopreventive and antitumor effects on prostate cancer. However, the molecular mechanism involved in curcumin's ability suppress cancer cell invasion, tumor growth, metastasis is not yet well understood. In this study, we have that curcumin can epidermal growth factor (EGF)- stimulated heregulin-stimulated PC-3 as androgen-induced LNCaP invasion. treatment significantly resulted reduced matrix metalloproteinase 9 activity downregulation of cellular...
The recent threats of influenza epidemics and pandemics have prioritized the development a universal vaccine that offers protection against wider variety infections. Here, we demonstrate genetically modified virus-like particle (VLP) vaccine, referred to as H5M2eN1-VLP, increased antigenic content NA induced rapid recall antibody HA2 after viral infection. As result, H5M2eN1-VLP vaccination elicited broad humoral immune response multiple proteins caused significant homologous RG-14 (H5N1)...