A5038615752- Prostate Cancer Treatment and Research
- Estrogen and related hormone effects
- Hormonal and reproductive studies
- Nuclear Receptors and Signaling
- Renal cell carcinoma treatment
- Bladder and Urothelial Cancer Treatments
- Sexual Differentiation and Disorders
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Cancer, Lipids, and Metabolism
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Prostate Cancer Diagnosis and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Retinoids in leukemia and cellular processes
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- RNA Research and Splicing
- Sperm and Testicular Function
University of Rochester Medical Center
2015-2025
China Medical University Hospital
2014-2025
China Medical University
2014-2025
Chengde Medical University
2025
University of Rochester
2012-2022
Tianjin Medical University
2011-2022
China Medical University
2012-2022
Shanghai Jiao Tong University
2011-2022
Shanghai Tenth People's Hospital
2022
Tongji University
2022
By using a cre-lox conditional knockout strategy, we report here the generation of androgen receptor (ARKO) mice. Phenotype analysis shows that ARKO male mice have female-like appearance and body weight. Their testes are 80% smaller serum testosterone concentrations lower than in wild-type (wt) Spermatogenesis is arrested at pachytene spermatocytes. The number size adipocytes also different between wt Cancellous bone volumes reduced compared with littermates. In addition, found average pups...
The androgen receptor (AR) is a member of the steroid superfamily that plays an important role in male sexual differentiation and prostate cell proliferation. Mutations or abnormal expression AR cancer can play key process changes from androgen-dependent to androgen-independent stage. Using yeast two-hybrid system, we were able isolate ligand-dependent AR-associated protein (ARA70), which functions as activator enhance transcriptional activity 10-fold presence 10(-10) M dihydrotestosterone...
Overexpression of the HER2/Neu protooncogene has been linked to progression breast cancer. Here we demonstrate that growth prostate cancer LNCaP cells can also be increased by stable transfection HER2/Neu. Using AG879, a inhibitor, and PD98059, MAP kinase as well phosphatase-1 (MPK-1), in assay, found could induce prostate-specific antigen (PSA), marker for cancer, through pathway at low androgen level. Reporter assays mammalian two-hybrid further suggest this HER2/Neu-induced receptor (AR)...
RNA and protein products encoded by the testosterone-repressed prostate message-2 gene (TRPM-2) are induced to high levels, coordinate with onset of cell death, in numerous rodent models inducible tissue damage. These include death initiated hormonal stimuli (prostate regression), pressure insult (renal atrophy after ureteral obstruction), developmental (necrosis interdigital tissue), cytotoxic injury (chemotherapeutic regression a tumor). Sequence analysis cDNA encoding TRPM-2 revealed its...
Androgens and the androgen receptor (AR) play important roles in male fertility, although detailed mechanisms, particularly how androgen/AR influences spermatogenesis particular cell types, remain unclear. Using a Cre-Lox conditional knockout strategy, we generated tissue-specific mouse with AR gene deleted only Sertoli cells (S-AR -/y ). Phenotype analyses show S-AR mice were indistinguishable from WT (B6 +/y ) exception of testes, which significantly atrophied. infertile, spermatogenic...
Whereas several apoptosis-related proteins have been linked to the antiapoptotic effects of Akt serine–threonine kinase, search continues explain signaling role in promoting cell survival via effects. Here, we demonstrate that phosphorylates androgen receptor (AR) at Ser-210 and Ser-790. A mutation AR results reversal Akt-mediated suppression transactivation. Activation phosphatidylinositol-3-OH kinase/Akt pathway target genes, such as p21, decrease androgen/AR-mediated apoptosis, which may...
BackgroundMales have a higher incidence of bladder cancer than females, but the reason remains unknown. Unlike prostate cancer, human is not generally considered to be dependent on hormone activity. We investigated possible involvement androgens and androgen receptor (AR) in cancer.
What's known on the subject? and What does study add? Steroid hormone receptor signals have been implicated in bladder tumourigenesis tumour progression. The expression of androgen and/or oestrogen receptors has assessed cancer, leading to conflicting data levels their relationship histopathological characteristics tumours. We simultaneously analyze three non‐neoplastic tissues as well primary metastatic specimens. Our demonstrate that status correlates with grades/stages patients’ outcomes....
Abstract While the antiandrogen enzalutamide (Enz) extends castration resistant prostate cancer (CRPC) patients’ survival an extra 4.8 months, it might also result in some adverse effects via inducing neuroendocrine differentiation (NED). Here we found that lncRNA-p21 is highly expressed NEPC patients derived xenograft tissues (NEPC-PDX). Results from cell lines and human clinical sample surveys revealed expression up-regulated Enz treatment could increase to induce NED. Mechanism dissection...
In PTEN-deficient prostate cancers, AKT signaling may be activated upon suppression of androgen receptor signaling. Activation as well NF-κB involves a key regulatory protein complex containing PHLPP, FKBP51 and IKKα. Here, we report critical role lncRNA PCAT1 in regulating the PHLPP/FKBP51/IKKα progression castration-resistant cancer (CRPC). Using database queries, bioinformatic analyses, RIP RNA pull-down assays, discovered validated that lncRNA-PCAT1 perturbs activates Expression is...
The roles of the androgen receptor (AR) in female fertility and ovarian function remain largely unknown. Here we report on generation mice lacking AR ( –/– ) resulting influences reproductive system. Female appear normal but show longer estrous cycles reduced fertility. ovaries from sexually mature females exhibited a marked reduction number corpora lutea. After superovulation treatment, produced fewer oocytes also showed During periovulatory period, an intensive granulosa apoptosis event...
Androgen receptor (AR) is a hormone-activated transcriptional factor that can bind to androgen response elements and regulates the transcription of target genes via mechanism presumably involves cofactors. We report here cloning novel AR coactivator ARA55 using yeast two-hybrid system. consists 444 amino acids with predicted molecular mass 55 kDa its sequence shows very high homology mouse hic5, TGF-β1-inducible gene. Yeast mammalian systems co-immunoprecipitation assays all prove in...
While all three coactivators ARA 70 , steroid receptor coactivator 1, and RAC3/ACTR can enhance androgen (AR) transcriptional activity at 1 nM dihydrotestosterone, we here demonstrate that only induce AR >30-fold in the presence of 10 17β-estradiol (E2), but not diethylstilbestrol. The significance this newly described E2-induced DU145 human prostate cancer cells was further strengthened by finding patients with Reifenstein partial-androgen-insensitive syndrome fail E2-AR-ARA pathway....
Modification by acetylation occurs at -amino lysine residues of histones and transcription factors.Unlike phosphorylation, a direct link between factor cellular growth or apoptosis has not been established.We show that the nuclear androgen receptor (AR), DNA-binding transcriptional regulator, is acetylated in vivo.The AR induced ligand dihydrotestosterone histone deacetylase (HDAC) inhibitors living cells.Direct augmented p300 binding vitro.Constructs mimicking neutral polar substitution (AR...