A5026534555- Prostate Cancer Treatment and Research
- Ubiquitin and proteasome pathways
- Cancer, Lipids, and Metabolism
- Estrogen and related hormone effects
- Cancer-related Molecular Pathways
- Mass Spectrometry Techniques and Applications
- Protein Tyrosine Phosphatases
- Hormonal and reproductive studies
- Cancer, Hypoxia, and Metabolism
- HER2/EGFR in Cancer Research
- Epigenetics and DNA Methylation
- 14-3-3 protein interactions
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- Hedgehog Signaling Pathway Studies
- Natural product bioactivities and synthesis
- Glycosylation and Glycoproteins Research
- Redox biology and oxidative stress
- Protein Degradation and Inhibitors
- Prostate Cancer Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Heat shock proteins research
- Bone health and treatments
- Mitochondrial Function and Pathology
- Macrophage Migration Inhibitory Factor
China United Network Communications Group (China)
2025
University of Nebraska Medical Center
2014-2024
Nebraska Medical Center
2007-2022
China Medical University Hospital
2020
China Medical University
2020
Kaohsiung Medical University
1994-2019
I-Shou University
2014
National Chung Hsing University
2013
Taichung Hospital
2013
Chang Gung University
2011
Overexpression of the HER2/Neu protooncogene has been linked to progression breast cancer. Here we demonstrate that growth prostate cancer LNCaP cells can also be increased by stable transfection HER2/Neu. Using AG879, a inhibitor, and PD98059, MAP kinase as well phosphatase-1 (MPK-1), in assay, found could induce prostate-specific antigen (PSA), marker for cancer, through pathway at low androgen level. Reporter assays mammalian two-hybrid further suggest this HER2/Neu-induced receptor (AR)...
Neuroendocrine (NE) cells are the minor cell populations in normal prostate epithelial compartments. During carcinogenesis, number of NE malignant lesions increases, correlating with its tumorigenicity and hormone-refractory growth. It is thus proposed that cancerous promote cancer (PCa) progression androgen-independent proliferation, although origin not clear. To investigate role we characterized three subclone lines–NE-1.3, NE-1.8 NE-1.9, which were transdifferentiated from...
Abstract BACKGROUND The acquisition of an androgen‐independent phenotype is the most serious issue prostate cancer treatment. Although several experimental cell models have been reported for studying androgen independence, they limited applications related to hormone‐refractory cancer. To investigate molecular mechanism growth cancer, we established a useful LNCaP model that resembles clinical scenario METHODS Androgen‐sensitive parental cells were continuously maintained in regular...
Abstract Anticancer drugs docetaxel and vinorelbine suppress cell growth by altering microtubule assembly activating the proapoptotic signal pathway. Vinorelbine have been approved for treating several advanced cancers. However, their efficacy in management of hormone‐refractory prostate cancer remains to be clarified. Microtubule damage some anticancer can activate ERK survival pathway, which conversely compromises chemotherapeutic efficacy. We analyzed effect inhibitors PD98059 U0126 on...
Because of the heterogeneous nature prostate cancer, identifying molecular mechanisms involved during transition from an androgen-sensitive to androgen-independent phenotype is very complex. An LNCaP cell model that recapitulates cancer progression, comprising early passage (LNCaP-C33) and late (LNCaP-C81) phenotypes, would help provide a better understanding such events. In this study, we examined genes expressed by LNCaP-C33 LNCaP-C81 cells using cDNA microarrays containing 1176 known...
Androgen plays a critical role in regulating the growth and differentiation of normal prostate epithelia, as well initial cancer cells. Nevertheless, carcinomas eventually become androgen-unresponsive, is refractory to hormonal therapy. To gain insight into mechanism involved this hormone-refractory phenomenon, we have examined potential androgen receptor (AR) that process. We investigated expression AR two prostate-specific androgen-responsive antigens, prostatic acid phosphatase (PAcP)...
Abstract The epidermal growth factor receptor (EGFR) and hedgehog cascades provide a critical role in prostate cancer progression contribute to the resistance clinical therapies disease relapse. Therefore, we evaluated, for first time, antiproliferative cytotoxic effects induced by combination of selective inhibitors EGFR tyrosine kinase smoothened signaling element, gefitinib cyclopamine, with current chemotherapeutic drug used clinics, docetaxel, on some metastatic cell lines....
Protein arginine methyltransferases (PRMTs) are proved to play vital roles in chromatin remodeling, RNA metabolism, and signal transduction. Aberrant regulation of PRMT activity is associated with various pathological states such as cancer cardiovascular disorders. Development application small molecule inhibitors will provide new avenues for therapeutic discovery. The combination pharmacophore-based virtual screening methods radioactive methylation assays provided six hits identified...
Abstract While better management of loco-regional prostate cancer (PC) has greatly improved survival, advanced PC remains a major cause deaths. Identification novel targetable pathways that contribute to tumor progression in could open new therapeutic options. The di-ganglioside GD2 is target FDA-approved antibody therapies neuroblastoma, but the role unexplored. Here, we show expressed small subpopulation cells subset patients and higher proportion metastatic tumors. Variable levels cell...
A disintegrin and metalloproteases (ADAMs) are proteins that contain both a metalloprotease domain have potential implications for the metastasis of human cancer cells via cell adhesion protease activities. In this study, we analyzed expression levels ADAM-9, ADAM-10 ADAM-17 (TNF-alpha converting enzyme, TACE), TIMP-3 (tissue inhibitor metalloproteinase-3) in prostatic tumor lines as well clinical patient materials (BPH tissue samples). Human (MDA PCa 2b, LNCaP-C33, -C51, -C81, -Pro5, -Ln3,...
Abstract Although the blockade of hedgehog cascade by using cyclopamine has been reported to inhibit growth some cancer cell types, few studies on mechanism which this drug alone or in combination with other cytotoxic agents induces its effect have reported. In our study, we evaluate, for first time, antiproliferative and effects induced a selective SMO inhibitor, epidermal factor receptor (EGFR) tyrosine kinase gefitinib metastatic prostate (PC) cells. The results revealed that cyclopamine,...
Abstract BACKGROUND The expression of prostate‐derived factor (PDF) is significantly elevated in human prostate tumors. We investigate the functional role and signaling PDF androgen receptor (AR)‐positive cancer cells. METHODS Transient or stable by cDNA transfection, antisense‐mediated gene silencing, media conditioned PDF‐elevated cells, antibody (Ab) neutralization were employed. RESULTS Elevated endogenous exogenous treatment PDF‐enriched associated with increased proliferation...
Background. Nosocomial infection with antibiotic-resistant strains is a major threat to critical care medicine. Selective decontamination of the digestive tract (SDD) one strategies used reduce ventilator-associated pneumonia and sepsis in critically ill patients. In present study, we performed pathogenic challenges transgenic milk-fed animal model test whether porcine lactoferrin (pLF) an effective SDD regimen.
G-protein-coupled receptor (GPCR)-stimulated androgen-independent activation of androgen (AR) contributes to acquisition a hormone-refractory phenotype by prostate cancer. We previously reported that regulator G-protein signaling (RGS) 2, an inhibitor GPCRs, inhibits AR (Cao et al., Oncogene 2006;25:3719-34). Here, we show reduced RGS2 protein expression in human cancer specimens compared adjacent normal or hyperplastic tissue. Methylation-specific PCR analysis and bisulfite sequencing...
Understanding cell proliferation regulation in hormone refractory prostate cancer may provide answers for novel solutions. Protein tyrosine phosphatases have been thought to key roles regulating and be involved oncogenesis, although our knowledge their functional human remain unknown. Human prostatic acid phosphatase (PAcP), a major epithelium, has shown function as neutral protein these cells. We evaluated the biological significance of cellular expression cells.Immunohistochemical testing...