A5052638149- Ubiquitin and proteasome pathways
- Sarcoma Diagnosis and Treatment
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- Virus-based gene therapy research
- Endoplasmic Reticulum Stress and Disease
- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Chronic Myeloid Leukemia Treatments
- DNA Repair Mechanisms
- Cancer Cells and Metastasis
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Planarian Biology and Electrostimulation
- Histone Deacetylase Inhibitors Research
- Glycosylation and Glycoproteins Research
- Lymphoma Diagnosis and Treatment
- RNA Research and Splicing
- Cellular transport and secretion
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related molecular mechanisms research
- Ion Channels and Receptors
- HER2/EGFR in Cancer Research
- Caveolin-1 and cellular processes
- Autophagy in Disease and Therapy
University of Nebraska Medical Center
2016-2025
Nebraska Medical Center
2012-2025
University of Nebraska at Omaha
2011-2023
Pediatrics and Genetics
2023
Temple University
2014
UConn Health
2014
Bioinks for 3D bioprinting of tumor models should not only meet printability requirements but also accurately maintain and support phenotypes surrounding cells to recapitulate key hallmarks. Collagen is a major extracellular matrix protein solid tumors, low viscosity collagen solution has made bioprinted cancer challenging. This work produces embedded, breast organoid using low-concentration I based bioinks. The biocompatible physically crosslinked silk fibroin hydrogel used generate the...
Janus kinase 2 (JAK2) is a central in hematopoietic stem/progenitor cells (HSPCs), and its uncontrolled activation prominent oncogenic driver of neoplasms. However, molecular mechanisms underlying the regulation JAK2 have remained elusive. Here we report that Casitas B-cell lymphoma (CBL) family E3 ubiquitin ligases down-regulate stability signaling via adaptor protein LNK/SH2B3. We demonstrated depletion CBL/CBL-B or LNK abrogated ubiquitination, extended half-life, enhanced cell growth...
The proto-oncogene Casitas b-lineage lymphoma (c-Cbl) is an adaptor protein with intrinsic E3 ubiquitin ligase activity that targets receptor and nonreceptor tyrosine kinases, resulting in their ubiquitination downregulation. However, the function of c-Cbl control cardiac currently unknown. In this study, we examined role myocyte death after myocardial ischemia.We show increased expression human ischemic dilated cardiomyopathy hearts response to pathological stress stimuli mice....
Abstract While better management of loco-regional prostate cancer (PC) has greatly improved survival, advanced PC remains a major cause deaths. Identification novel targetable pathways that contribute to tumor progression in could open new therapeutic options. The di-ganglioside GD2 is target FDA-approved antibody therapies neuroblastoma, but the role unexplored. Here, we show expressed small subpopulation cells subset patients and higher proportion metastatic tumors. Variable levels cell...
CHIP/STUB1 ubiquitin ligase is a negative co-chaperone for HSP90/HSC70, and its expression reduced or lost in several cancers, including breast cancer. Using an extensive well-annotated cancer tissue collection, we identified the loss of nuclear but not cytoplasmic CHIP to predict more aggressive tumorigenesis shorter patient survival, with two thirds ErbB2+ triple-negative cancers (TNBC) one third ER+ cancers. Reduced was seen patient-derived xenograft tumors TNBC cell lines. Ectopic lines...
Mayumi Naramura 1,2 , Scott Nadeau Bhopal Mohapatra 1,3 Gulzar Ahmad 1 Chandrani Mukhopadhyay Martin Sattler 5 Srikumar M Raja Amarnath Natarajan 1,4 Vimla Band Hamid 1,2,3 Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 2 Department Genetics, Cell Biology Anatomy, College Medicine, 3 Biochemistry & Molecular Biology, 4 Pharmaceutical Sciences, Pharmacy, Dana Farber Institute, Harvard School, Boston, MA Keywords: Cbl, E3 ubiquitin...
Abstract Members of the four-member C-terminal EPS15-Homology Domain-containing (EHD) protein family play crucial roles in endocytic recycling cell surface receptors from endosomes to plasma membrane. In this study, we show that Ehd1 gene knockout mice on a predominantly B6 background is embryonic lethal. -null embryos die at mid-gestation with failure complete key developmental processes including neural tube closure, axial turning and patterning tube. We found display short stubby cilia...
With nearly all cancer deaths a result of metastasis, elucidating novel pro-metastatic cellular adaptations could provide new therapeutic targets. Here, we show that overexpression the EPS15-Homology Domain-containing 2 (EHD2) protein in large subset breast cancers (BCs), especially triple-negative (TNBC) and HER2+ subtypes, correlates with shorter patient survival. The mRNAs for EHD2 Caveolin-1/2, structural components caveolae, co-overexpression across tumors, predicting survival...
The ecdysoneless (ECD) mRNA and protein are overexpressed in breast cancer (BC), its overexpression correlates with poor prognosis short patient survival, particularly ERBB2/HER2-positive BC. This study investigates the co-operative oncogenic mechanism of ECD ERBB2 by deriving transgenic mice overexpressing and/or (huHER2) mammary epithelium under MMTV promoter, as well human immortal epithelial cell lines (hMECs) ERBB2. While huHER2Tg developed more homogenous solid nodular carcinomas,...
Abstract Androgen receptor (AR)-mediated signaling is essential for PC tumorigenesis. In TCGA database we observed a positive correlation between ECD and AR expression. Consistently, Dihydrotestosterone (DHT) treatment of cell lines increased mRNA protein levels, knockdown (KD) reduced Bioinformatic analysis predicted three consensus androgen response elements in the promoter, DHT occupancy at enhanced promoter activity. Enzalutamide decreased knockout (KO) cells oncogenic traits, suggesting...
Abstract Introduction: Prostate cancer (PC) is the most diagnosed in males. Androgen receptor (AR) signaling essential for PC progression. ECD (Ecdysoneless) protein participates cell cycle progression and survival overexpressed non-PC cancers. Current study explored role function of Methods: TCGA Adenocarcinoma database was used to assess correlation between AR expression, ECD’s expression dataset. Bioinformatic analysis performed predict androgen response elements promoter. Chromatin...
Abstract Triple-negative breast cancer (TNBC) has a relatively high metastasis rate as compared to other subtypes. Metastasis and tumor recurrence remain major challenges for TNBC patients, resulting in poor prognosis. Therefore, it is imperative identify elucidate the molecular mechanisms that drive allow development of new therapeutic agents inhibit improve patients’ outcomes. Herein, we uncovered mechanistic connection between multifunctional DNA repair protein APE1 G-quadruplex (G4),...
Abstract Introduction: Recurrence, therapy resistance and metastasis remain major challenges in cancer therapy, accounting for nearly all deaths from cancer. The inability of current therapies to eliminate stem cells (CSCs) is widely believed underlie these challenges. Targeting molecular pathways that help maintain the CSCs could open new avenues devise durable therapies. CBL-family E3 ubiquitin ligases (CBL, CBL-B CBL-C) attenuate tyrosine kinase signaling through ubiquitin-dependent...
Abstract Background: Breast cancer (BC) is a heterogenous disease, with estrogen receptor-positive (ER+) BC accounting for over 70% of cases. ER+ depend on signaling oncogenesis and metastasis. Even though treatments targeting ER pathway have improved patient’s outcome, factors driving progression particularly in metastatic remain unclear. One these Ecdysoneless (ECD), highly conserved protein from yeast to human. ECD expression correlates expression, overexpression observed poor patient...
Abstract Background: Triple-negative breast cancer (TNBC) is an aggressive and highly metastatic subtype characterized by poor therapeutic outcomes. Increased invasive capabilities expose tumor cells to higher plasma membrane stress resulting in frequent breaches that are incompatible with cell survival invasiveness. Upregulation of repair mechanisms has been shown counteract this inherent vulnerability targeting such represents a novel potential strategy. Previous studies have involves...
Abstract Introduction: The mammalian ortholog of the Drosophila ecdysoneless (ECD) protein regulates cell cycle progression and survival. ECD levels are overexpressed in breast cancer (BC), its overexpression correlates with poor prognosis short patient survival, particularly ERBB2-positive BC. This study explores cooperative oncogenic mechanisms ERBB2 to enhance progression. Methods: We generated immortal human mammary immortalized epithelial lines (hMECs) overexpressing ECD, ERBB2, or...
// Srikumar M. Raja 1,6,* , Swapnil S. Desale 2,* Bhopal Mohapatra 1 Haitao Luan Kruti Soni 2 Jinjin Zhang Matthew A. Storck Dan Feng Timothy Bielecki Vimla Band 3 Samuel Cohen 5 Tatiana K. Bronich and Hamid 1,2,3,4 Eppley Institute for Research in Cancer Allied Diseases, Omaha, Nebraska, USA Department of Pharmaceutical Sciences Center Drug Delivery Nanomedicine, Genetics, Cell Biology Anatomy, College Medicine, University Nebraska Medical Center, 4 Departments Biochemistry Molecular...
// Wei An 1, 2 , Scott A. Nadeau Bhopal C. Mohapatra 3 Dan Feng 1 Neha Zutshi 4 Matthew D. Storck Priyanka Arya James E. Talmadge Jane L. Meza 5 Vimla Band 2, 6 Hamid 3, 4, Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198, USA Departments Genetics, Cell Biology & Anatomy, Biochemistry Molecular Biology, Pathology Microbiology, College Medicine, Public Health, Fred Pamela Buffet Correspondence to: Band, e-mail: hband@unmc.edu...
Fluorouracil (5-FU) remains a first-line chemotherapeutic agent for colorectal cancer. However, subset of cancer patients who have defective mismatch-repair (dMMR) pathway show resistance to 5-FU. Here, we demonstrate that the efficacy 5-FU in dMMR cells is largely dependent on DNA base excision repair (BER) pathway. Downregulation APE1, key enzyme BER pathway, decreases IC50 by 10-fold. Furthermore, discover facilitates chromatin transcription (FACT) complex via promoting recruitment and...
Overexpression of the EPS15 Homology Domain containing 1 (EHD1) protein has been linked to tumorigenesis but whether its core function as a regulator intracellular traffic cell surface receptors plays role in oncogenesis remains unknown. We establish that EHD1 is overexpressed Ewing sarcoma (EWS), with high mRNA expression specifying shorter patient survival. ShRNA-knockdown and CRISPR-knockout mouse Ehd1 rescue established requirement for metastasis. RTK antibody arrays identified IGF-1R...