A5001166654- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Electrospun Nanofibers in Biomedical Applications
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Developmental Biology and Gene Regulation
- Mesenchymal stem cell research
- Advanced Sensor and Energy Harvesting Materials
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Immune Response and Inflammation
- RNA modifications and cancer
- Pain Mechanisms and Treatments
- Hydrogels: synthesis, properties, applications
- Shoulder Injury and Treatment
- Surgical Sutures and Adhesives
- Radiopharmaceutical Chemistry and Applications
- Nerve Injury and Rehabilitation
- Connexins and lens biology
- Heat shock proteins research
- Extracellular vesicles in disease
- Graphene and Nanomaterials Applications
- Ubiquitin and proteasome pathways
- Neuroendocrine Tumor Research Advances
University of Nebraska Medical Center
2015-2025
Guangzhou Sport University
2024
Nanjing Medical University
2019-2021
Nanjing Brain Hospital
2021
Nanjing Chest Hospital
2021
Guangzhou University of Chinese Medicine
2012-2021
Jiangsu Province Hospital
2012-2019
Nankai University
2016-2019
Nebraska Medical Center
2013-2016
Molecular Oncology (United States)
2011
Abstract The vascular endothelial growth factor (VEGF) plays a key role in tumor angiogenesis. However, clinical trials targeting the VEGF pathway are often ineffective, suggesting that other factors/pathways also important We have previously shown Notch ligand Delta-like 4 (DLL4) is up-regulated vasculature. Here, we show DLL4, when expressed cells, functions as negative regulator of angiogenesis by reducing number blood vessels all five types xenografts, but acts positive driver for two...
Due to their intrinsic injectable and self-healing characteristics, dynamic hydrogels, based on covalent bonds, have gained a great attention. In this study, novel hydrogel the boronic ester bond is facilely developed using phenylboronic acid-modified hyaluronic acid (HA-PBA) plant-derived polyphenol-tannic (TA). The gelated quickly under mild conditions had favorable viscoelastic properties with good shear-thinning capabilities. Moreover, simultaneous utilization of TA as reductant for...
Postoperative abdominal adhesions are a common problem after surgery and can produce serious complications. Current antiadhesive strategies focus mostly on physical barriers unsatisfactory inefficient. In this study, we designed synthesized advanced injectable cream-like hydrogels with multiple functionalities, including rapid gelation, self-healing, antioxidation, anti-inflammation, anti-cell adhesion. The multifunctional were facilely formed by the conjugation reaction of...
The lack of both digital light processing (DLP) compatible and biocompatible photopolymers, along with inappropriate material properties required for wearable sensor applications, substantially hinders the employment DLP 3D printing in fabrication multifunctional hydrogels. Herein, we discovered implemented a photoreactive poloxamer derivative, Pluronic F-127 diacrylate, which overcomes these limitations is optimized to achieve printed micelle-based hydrogels high structural complexity,...
Resistance to VEGF inhibitors is emerging as a major clinical problem. Notch signaling has been implicated in tumor angiogenesis. Therefore, investigate mechanisms of resistance angiogenesis inhibitors, we transduced human glioblastoma cells with retroviruses encoding delta-like ligand 4 (DLL4), grew them xenografts and then treated the murine hosts VEGF-A inhibitor bevacizumab. We found that DLL4-mediated bevacizumab vivo. The large vessels induced by DLL4-Notch increased blood supply were...
Adipose-derived mesenchymal stem/stromal cells (ADMSC) are one of the major stromal in breast cancer microenvironment that promote progression. Previous studies on effects ADMSC metastasis and drug resistance, using two-dimensional (2D) cultures, remained inconclusive. In present study, we compared cocultured human epidermal receptor 2 positive primary (21PT) 2D three-dimensional (3D) cultures then examined their response to doxorubicin (DOX). We 3D bioprinted constructs with middle edge...
Functional reconstruction of craniomaxillofacial defects is challenging, especially for the patients who suffer from traumatic injury, cranioplasty, and oncologic surgery. Three-dimensional (3D) printing/bioprinting technologies provide a promising tool to fabricate bone tissue engineering constructs with complex architectures bioactive components. In this study, we implemented multi-material 3D printing printed PCL/hydrogel composite scaffolds loaded dual small molecules (i.e. resveratrol...
Repairing massive rotator cuff tendon defects remains a challenge due to the high retear rate after surgical intervention. 3D printing has emerged as promising technique that enables fabrication of engineered tissues with heterogeneous structures and mechanical properties, well controllable microenvironments for regeneration. In this study, we developed new strategy repair by combining printed scaffold polylactic-
Abstract In the past decade, cartilage tissue engineering has arisen as a promising therapeutic option for degenerative joint diseases, such osteoarthritis, in hope of restoring structure and physiological functions. Hydrogels are biomaterials developing engineered scaffolds regeneration. However, hydrogel-delivered mesenchymal stem cells or chondrocytes could be exposed to elevated levels reactive oxygen species (ROS) inflammatory microenvironment after being implanted into injured joints,...
Bioinks for 3D bioprinting of tumor models should not only meet printability requirements but also accurately maintain and support phenotypes surrounding cells to recapitulate key hallmarks. Collagen is a major extracellular matrix protein solid tumors, low viscosity collagen solution has made bioprinted cancer challenging. This work produces embedded, breast organoid using low-concentration I based bioinks. The biocompatible physically crosslinked silk fibroin hydrogel used generate the...
Peripheral nerve regeneration remains a significant clinical challenge due to the unsatisfactory functional recovery and public health burden. Exosomes, especially those derived from mesenchymal stem cells (MSCs), are promising as potential cell-free therapeutics gene therapy vehicles for promoting neural regeneration. In this study, we reported differentiation of human adipose MSCs (hADMSCs) towards Schwann cell (SC) phenotype (hADMSC-SCs) then isolated exosomes hADMSCs with without (i.e.,...
Abstract Peripheral nerve transection has a high prevalence and results in functional loss of affected limbs. The current clinical treatment using suture anastomosis significantly limits recovery due to severe inflammation, secondary damage, fibrosis. Fibrin glue, commercial adhesive as an alternative, avoids damage but suffers from poor adhesion strength. To address their limitations, highly efficacious based on dual‐cross‐linking dopamine‐isothiocyanate modified hyaluronic acid...
SAD1/UNC84 domain protein-2 (SUN2) plays a tumor suppressor role in various types of cancer by inhibiting cell proliferation, migration and promoting apoptosis. However, the post-translational regulation SUN2 cellular mechanism responsible for its proteasomal degradation remains largely unknown. Here, we show that FBXO2, an E3 ubiquitin ligase F-box proteins (FBPs) family targets glycosylated ubiquitination via ubiquitin-proteasome system (UPS). By integrating Cancer Genome Atlas (TCGA),...
Solid tumors are 3D assemblies of cancer cells, together with multiple stromal cell types within an extracellular matrix. Yet, the vast majority cell-based studies to characterize oncogenesis and discovery new anti-cancer drugs is conducted using conventional 2D monolayer culture systems, where cells grown on plastic substratum under normoxic environments. In current study, we generated breast platform consists photocrosslinkable hydrogels encapsulated isogenic primary (21PT) a metastatic...
Electrospinning provides a simple and convenient method to fabricate nanofibrous meshes. However, the nanofiber productivity is often limited laboratory scale, which cannot satisfy requirements of practical application. In this study, we developed novel needleless electrospinning spinneret based on double-ring slit drug-loaded contrast conventional single-needle spinneret, our needless can significantly improve due simultaneous formation multiple jets during electrospinning. Curcumin-loaded...