A5034678410- Urinary Bladder and Prostate Research
- Prostate Cancer Treatment and Research
- Hormonal and reproductive studies
- Sexual function and dysfunction studies
- Prostate Cancer Diagnosis and Treatment
- Sperm and Testicular Function
- Urological Disorders and Treatments
- Estrogen and related hormone effects
- Sexual Differentiation and Disorders
- Renal and related cancers
- Pelvic floor disorders treatments
- Bladder and Urothelial Cancer Treatments
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Genital Health and Disease
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Pediatric Urology and Nephrology Studies
- Cancer, Lipids, and Metabolism
- Urologic and reproductive health conditions
- Kidney Stones and Urolithiasis Treatments
- Testicular diseases and treatments
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- Renal and Vascular Pathologies
Liaoning Cancer Hospital & Institute
2025
China Medical University
2025
National Taiwan University Hospital
2015-2024
National Taiwan University
2007-2023
Far Eastern Memorial Hospital
2023
Wilmington University
2023
Incyte (United States)
2015-2023
Taipei City Government
2019
University of Rochester Medical Center
1999-2017
Taipei Medical University-Shuang Ho Hospital
2017
Immunophilins, a family of proteins that exhibit rotamase (peptidyl-prolyl cis-trans isomerase) activity in vitro, are expressed many organisms and most tissues. Although some immunophilins can mediate the immunosuppressive actions FK506, rapamycin, cyclosporin A, physiological role unligated is not known. A 59-kilodalton member FK506- rapamycin-binding class was found to associate absence these drugs with two heat shock (hsp90 hsp70) glucocorticoid receptor (GR). Together, make up inactive...
Androgen receptor (AR) is a hormone-activated transcriptional factor that can bind to androgen response elements and regulates the transcription of target genes via mechanism presumably involves cofactors. We report here cloning novel AR coactivator ARA55 using yeast two-hybrid system. consists 444 amino acids with predicted molecular mass 55 kDa its sequence shows very high homology mouse hic5, TGF-β1-inducible gene. Yeast mammalian systems co-immunoprecipitation assays all prove in...
No AccessJournal of UrologyAdult Urology1 Jun 2013Epidemiology Upper Urinary Tract Stone Disease in a Taiwanese Population: A Nationwide, Population Based Study Wei-Yi Huang, Yu-Fen Chen, Stacey Carter, Hong-Chiang Chang, Chung-Fu Lan, and Kuo-How Huang HuangWei-Yi Institute Health Welfare Policy, National Yang-Ming University, Taipei, Taiwan, Republic China Insurance Medical Expenditure Negotiation, , ChenYu-Fen Chen Dispute Mediation Committees, Department Health, Executive Yuan, Nursing,...
Early clinical studies suggested infiltrating T cells might be associated with poor outcomes in prostate cancer (PCa) patients. The detailed mechanisms how contribute to PCa progression, however, remained unclear. Here, we found have a better capacity recruit more CD4(+) than the surrounding normal via secreting chemokines‐CXCL9. consequences of recruited then lead enhance cell invasion. Mechanism dissection revealed that function through modulation FGF11→miRNA‐541 signals suppress androgen...
Abstract Epidemiologic studies suggested that vitamin E has a protective effect against prostate cancer. We showed here tocopherol-associated protein (TAP), E–binding protein, promoted uptake and facilitated antiproliferation in cancer cells. Interestingly, without treatment, overexpression of TAP cells significantly suppressed cell growth; knockdown endogenous by small interfering RNA (siRNA) nonmalignant HPr-1 increased growth. Further mechanism dissection the tumor suppressor function was...
Estrogens have been linked to a higher female incidence of autoimmune diseases. The role androgen and the receptor (AR) in diseases, however, remains unclear. Here we report that lack AR B cells different strains mice, namely general knockout, cell-specific naturally occurring testicular feminization mutation AR-mutant as well castrated wild-type results increased blood bone marrow. Analysis targeted together with marrow transplantation using Rag1(-/-) recipients, overexpression retrovirally...
Abstract BACKGROUND Androgens and the androgen receptor (AR) play important roles in development of male urogenital organs. We previously found that mice with total AR knockout (ARKO) epithelial ARKO failed to develop normal prostate loss differentiation. have recently knocked out gene smooth muscle cells reduced luminal infolding IGF‐1 production mouse prostate. However, stromal fibroblasts remain unclear. METHODS To further probe fibroblast development, we generated tissue‐selective...
Abstract BACKGROUND Androgens and the androgen receptor (AR) play critical roles in prostate development via mesenchymal–epithelial interactions. Smooth muscle cells (SMC), differentiated from mesenchyme, are one of basic components stroma. However, smooth AR still obscure. METHODS We established selective knockout (SM‐ARKO) mouse model using Cre‐loxP system, confirmed ARKO efficiency at RNA, DNA protein levels. Then, we observed morphology changes, determined epithelial proliferation,...
The recently developed anti‐androgen enzalutamide also known as (MDV3100) has the advantage to prolong by 4.8 months survival of castration resistant prostate cancer (CRPC) patients. However, mechanisms behind potential side effects involving induction (PCa) neuroendocrine (NE) differentiation remain unclear. Here we found PCa cells could recruit more mast than normal epithelial cells, and (or casodex) treatment further increase such recruitment that resulted in promoting NE differentiation....
High fat dietary intake may increase the risk of prostate cancer (PCa). Pre-adipocytes, one basic components in tumor microenvironment (TME), are capable differentiating into adipose tissues and play key roles to affect PCa progression. Here we found pre-adipocytes could be recruited more easily than its surrounding normal tissue. In vitro co-culture system also confirmed has a better capacity recruit pre-adipocytes. The consequences recruiting then cell invasion. Mechanism dissection...
BACKGROUND Although prostate carcinoma remains a rare disease among Chinese men, its incidence is on the rise. The authors conducted hospital-based case–control study to identify risk factors for in northern Taiwan. METHODS Patients at selected veterans hospital or 2 military hospitals who were newly diagnosed with between August 1995 and July 1996 included as cases (n = 90). Controls 180) comprised noncancer patients treated emergency rooms departments other than those of urology cardiology...
Human prostate cancer (PCa) and epithelial cells predominantly express estrogen receptor (ER) β, but not ERα. ERβ might utilize various ER coregulators to mediate the E2-signaling pathway in PCa. Here, we identified coiled-coil domain containing 62 (CCDC62)/ERAP75 as a novel coactivator. CCDC62/ERAP75 is widely expressed PCa cell lines has low expression MCF7 cells. Both vitro vivo interaction assays using mammalian two-hybrid, glutathione S-transferase pull-down coimmunoprecipitation...
Abstract Aim To determine the incidence rate and characteristics in patients with testicular torsion T aiwan using a nationwide insurance database. Methods This study utilizes subset of national health research database, which contains data all paid medical benefit claims over period 1997–2010 for 1 000 beneficiaries 2005. We analysed male subjects younger than 25 years diagnosis torsion. Results A total 86 were included. Among them, 22 (25.6%) underwent orchiectomies 64 (74.4%)...
Abstract Cisplatin-based chemotherapy is the primary treatment for metastatic bladder urothelial carcinoma. However, response rate only 40–65%. This study investigated anti-tumor effect and underlying mechanisms of combination cisplatin NEDD8-activating enzyme inhibitor MLN4924 in human The exerted synergistic cytotoxicity on two high-grade carcinoma cell lines, NTUB1 T24 (combination index <1). also potentiated cisplatin-induced apoptosis activation caspase-3 -7, phospho-histone H2A.X...
Early reports showed that androgen receptor (AR) NH2- and COOH-terminal (N-C) interaction was important for full AR function. However, the influence of these interactions on in vivo effects remains unclear. Here we tested some AR-associated peptides coregulators to determine their influences N-C interaction, transactivation, coregulator The results coactivators such as ARA70N, gelsolin, ARA54, SRC-1 can enhance transactivation but differential interaction. In contrast, corepressors ARA67...
The ligand-bound androgen receptor (AR) regulates target genes via a mechanism involving coregulators such as receptor-associated 54 (ARA54). We investigated whether the interruption of AR coregulator function could lead to down-regulation activity. Using in vitro mutagenesis and yeast two-hybrid screening assay, we have isolated mutant ARA54 (mt-ARA54) carrying point mutation at amino acid 472 changing glutamic lysine, which acts dominant-negative inhibitor transactivation. In transient...
Abstract Background This study was undertaken to determine the optimal cut-off value for FSH predict presence of spermatogenesis in patients with non-obstructive azoospermia. Methods A total 206 azoospermic men were enrolled this prospective study. By using receiver operating characteristic (ROC) curves, we determined and evaluated whether test could adequately successful sperm retrieval. Results There 108 who had evidence (group A) achieved success Another 98 B) failed The mean serum group...
Background Human sperm quality fluctuates over time. Therefore, it is crucial for couples preparing natural pregnancy to monitor motility. Objective This study verified the performance of an artificial intelligence–based image recognition and cloud computing motility testing system (Bemaner, Createcare) composed microscope microfluidic modules designed adapt different types smartphones. Methods Sperm videos were captured uploaded with app. Analysis was performed by algorithm then results...