A5067497938- Thyroid Disorders and Treatments
- Cell Adhesion Molecules Research
- Estrogen and related hormone effects
- Growth Hormone and Insulin-like Growth Factors
- Sirtuins and Resveratrol in Medicine
- interferon and immune responses
- Cancer-related Molecular Pathways
- Cytokine Signaling Pathways and Interactions
- Retinoids in leukemia and cellular processes
- Cancer, Hypoxia, and Metabolism
- Radiopharmaceutical Chemistry and Applications
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- S100 Proteins and Annexins
- Protein Kinase Regulation and GTPase Signaling
- Prostate Cancer Treatment and Research
- Thyroid Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Cancer, Stress, Anesthesia, and Immune Response
- Immunotherapy and Immune Responses
- Regulation of Appetite and Obesity
- NF-κB Signaling Pathways
- Peptidase Inhibition and Analysis
- Inflammatory mediators and NSAID effects
Albany College of Pharmacy and Health Sciences
2016-2025
Taipei Medical University
2016-2025
Taipei Medical University Hospital
2017-2025
Wan Fang Hospital
2019-2025
Albany Stratton VA Medical Center Albany
1997-2023
Albany Research Institute
2005-2023
Albany Medical Center Hospital
1998-2022
National Sun Yat-sen University
2022
University of California, Davis
2022
Academia Sinica
1986-2021
Integrin alpha(V)beta(3) is a heterodimeric plasma membrane protein whose several extracellular matrix ligands contain an RGD recognition sequence. This study identifies integrin as cell surface receptor for thyroid hormone [L-T(4) (T(4))] and the initiation site T(4)-induced activation of intracellular signaling cascades. dissociably binds radiolabeled T(4) with high affinity, this binding displaced by tetraiodothyroacetic acid, antibodies, peptide. CV-1 cells lack nuclear receptor, but...
Thyroid hormone [l-thyroxine (T 4 )] rapidly induced phosphorylation and nuclear translocation (activation) of mitogen-activated protein kinase (MAPK) in HeLa CV-1 cells the absence cytokine or growth factor. A pertussis toxin-sensitive guanosine 5′- O-(3-thiotriphosphate)-sensitive cell surface mechanism responsive to T agarose-T , suggesting a G protein-coupled receptor, was implicated. Cells depleted MAPK treated with pathway inhibitors showed reduced activation signal transducer...
3,5,3'-Triiodo-l-thyronine (T(3)), but not l-thyroxine (T(4)), activated Src kinase and, downstream, phosphatidylinositol 3-kinase (PI3-kinase) by means of an alpha(v)beta(3) integrin receptor on human glioblastoma U-87 MG cells. Although both T(3) and T(4) stimulated extracellular signal-regulated (ERK) 1/2, ERK1/2 did contribute to T(3)-induced or PI3-kinase activation, inhibitor PI3-kinase, LY-294002, block activation physiological concentrations T(4). Thus the kinase, signaling cascades...
The effects of thyroid hormone analogues on modulation angiogenesis have been studied in the chick chorioallantoic membrane model. Generation new blood vessels from existing was increased 3-fold by either l-thyroxine (T4; 10(-7) mol/L) or 3,5,3'-triiodo-l-thyronine (10(-9) mol/L). T4-agarose reproduced T4, and tetraiodothyroacetic acid (tetrac) inhibited both T4 T4-agarose. Tetrac itself inactive is known to block actions signal transduction that are initiated at plasma membrane. basic...
Activated nongenomically byl-thyroxine (T<sub>4</sub>), mitogen-activated protein kinase (MAPK) complexed in 10–20 min with endogenous nuclear thyroid hormone receptor (TRβ1 or TR) fractions of 293T cells, resulting serine phosphorylation TR. Treatment cells the MAPK inhibitor, PD 98059, prevented both T<sub>4</sub>-induced MAPK-TR co-immunoprecipitation and T<sub>4</sub> treatment caused dissociation TR SMRT (silencing mediator retinoid receptor), an effect also inhibited by 98059...
Abstract Activated by thyroid hormone, the MAPK (ERK1/2) signaling pathway causes serine phosphorylation of several nucleoproteins, including nuclear hormone receptor β1. Because estrogen can activate and cause MAPK-dependent (ER)α, we studied whether also promoted MAPK-mediated ERα phosphorylation. Human breast cancer (MCF-7) cells were incubated with physiological concentrations l-T4 or 17β-estradiol (E2) for 15 min to 24 h, serine-118-phosphorylated identified Western blotting....
Early studies demonstrated that whole-body androgen receptor (AR)–knockout mice with hypogonadism exhibit insulin resistance. However, details about the mechanisms underlying how androgen/AR signaling regulates sensitivity in individual organs remain unclear. We therefore generated hepatic AR-knockout (H-AR−/y) and found male H-AR−/y mice, but not female H-AR−/− fed a high-fat diet developed steatosis resistance, aging chow exhibited moderate steatosis. hypothesized increased obese resulted...
Two papillary thyroid carcinoma (PTC) and two follicular (FTC) cell lines treated with resveratrol (RV), 1-10 microM, showed activation nuclear translocation of MAPK (extracellular signal-regulated kinase 1/2). Cellular abundance the oncogene suppressor protein p53, serine phosphorylation c-fos, c-jun, p21 mRNAs were also increased by RV. Inhibition pathway either H-ras antisense transfection or PD 98059, an inhibitor, blocked these RV-induced effects. Addition pifithrin-alpha, a specific...
Using a Cre-Lox conditional knockout strategy, we generated germ cell-specific androgen receptor (AR) mouse (G-AR −/y ) with normal spermatogenesis. Sperm count and motility in epididymis from AR mice are similar to that of WT +/y mice. Furthermore, fertility tests show there was no difference fertility, almost 100% female pups sired by G-AR males younger than 15 weeks carried the deleted allele, suggesting efficient occurred cells during meiosis. Together, these data provide vivo evidence...
Human orbital fibroblasts play a putative role in the pathogenesis of thyroid-associated ophthalmopathy (TAO). We hypothesize that hyaluronan accumulation and inflammation TAO derive from enhanced biosynthetic activities fibroblasts. CD40, member tumor necrosis factor-α receptor superfamily, is critical signaling molecule expressed by B lymphocytes. Engagement CD40 with CD154 or ligand results activation target genes. Orbital also display CD40. Here we report engagement leads to substantial...
Under continuous illumination this unicellular aerobic cyanobacterium fixes dinitrogen continuously at a variable and usually low rate. Exposure of the culture to diurnal light/dark cycles invariably results in virtual restriction nitrogenase activity dark periods. The rhythmic fixation pattern becomes truely endogenous cycle which persists for least 4 days with decreasing magnitude on exposing illumination. free running time rhythm appears decrease from an initial 26 h 22 course days. This...
To examine the role of androgen receptor (AR) in Sertoli cells (SC), we used a SC-specific AR knockout (S-AR−/y) mouse to further evaluate chronological changes seminiferous tubules and molecular mechanisms SC androgen/AR signals on spermatogenesis. Testes morphology began changing as early postnatal day (PD) 10.5 wild-type (WT), but not S-AR−/y mice. After puberty (PD 50), nuclei WT testes migrated basal area epithelium; however, testes, were disarranged dislocated. Results from electron...
Abstract Recent evidence suggests that the thyroid hormone l-thyroxine (T4) stimulates growth of cancer cells via a plasma membrane receptor on integrin αVβ3. The contribution this recently described for and receptor-based stimulation cellular mitogen-activated protein kinase [MAPK; extracellular signal-regulated 1/2 (ERK1/2)] activity, to enhancement cell proliferation by was quantitated functionally immunologic means in three glioma lines exposed T4. At concentrations 1 100 nmol/L, T4...
Neutrophils, the major phagocytes that form first line of cell-mediated defense against microbial infection, are produced in bone marrow and released into circulation response to granulocyte-colony stimulating factor (G-CSF). Here, we report androgen receptor knockout (ARKO) mice neutropenic susceptible acute bacterial whereas castration only results moderate neutrophil reduction humans. Androgen supplement can restore counts via stabilizing AR castrated mice, but not ARKO testicular...
Epidemiological evidence suggests that sex differences exist in type 2 diabetes. Men seem to be more susceptible than women the consequences of obesity and sedentary lifestyle, possibly because insulin sensitivity regional body fat deposition. Thus, lacking androgen receptor (AR) male individuals may promote resistance. To determine whether AR contributes vivo resistance, an knockout model (AR(-/y)) was used study correlation between Progressive reduced impaired glucose tolerance were seen...
Resveratrol (Calbiochem, La Jolla, California) is a naturally occurring stilbene reported to cause apoptosis in various cultured cancer cells. In the current study effect of resveratrol was determined androgen insensitive DU 145 prostate cell line. Induction and activation related signal transduction pathways were measured.DU cells treated with measured by determining nucleosome content. Activation mitogen activated protein kinase (MAPK) (extracellular signal-regulated 1/2), p53 content...
Androgens and the androgen receptor (AR) play important roles in testes. Previously we have shown that male total AR knockout (T-AR-/y) mice revealed incomplete germ cell development lowered serum testosterone levels, which resulted azoospermia infertility. However, consequences of loss particular types testicular cells remain unclear. Using a Cre-loxP conditional strategy, generated tissue-selective mouse with gene deleted testis peritubular myoid (PM-AR-/y). Phenotype analyses showed...
Resveratrol is a naturally occurring polyphenol, which causes apoptosis in cultured cancer cells. We describe cell surface resveratrol receptor on the extracellular domain of hetero-dimeric alphaVbeta3 integrin MCF-7 human breast This linked to induction by extracellular-regulated kinases 1 and 2 (ERK1/2)- serine-15-p53-dependent phosphorylation leading apoptosis. The near Arg-Gly-Asp (RGD) recognition site integrin; an integrin-binding RGD peptide inhibits ERK1/2- p53-dependent Antibody...