Store-operated Ca2+ entry (SOCE) encoded by Orai1 proteins is a ubiquitous Ca2+-selective conductance involved in cellular proliferation and migration. We recently described up-regulation of Orai3 channels that selectively mediate SOCE estrogen receptor α-expressing (ERα+) breast cancer cells. However, the connection between ERα role tumorigenesis remain unknown. Here, we show knockdown decreases mRNA (by ~63%) protein ~44%) with no effect on Orai1. Orai3-mediated ~43%) corresponding...

Resveratrol (Calbiochem, La Jolla, California) is a naturally occurring stilbene reported to cause apoptosis in various cultured cancer cells. In the current study effect of resveratrol was determined androgen insensitive DU 145 prostate cell line. Induction and activation related signal transduction pathways were measured.DU cells treated with measured by determining nucleosome content. Activation mitogen activated protein kinase (MAPK) (extracellular signal-regulated 1/2), p53 content...

Addition of normal C57BL/6 mouse bone marrow cells to an in vitro culture spleen and allogeneic P815-Y tumor inhibited the development cell-mediated immunity. Bacillus Calmette-Guérin (BCG) enhanced suppressive activity these as early 2 days after its intravenous administration donor mice elicited similar by 7 days. Concomitant with appearance suppressor there was a decrease cell number increase number. While failed inhibit immunization, from thymectomized, irradiated, marrow-reconstituted...

Abstract Foci, nodules of cellular overgrowth, that appear after confluence are an in vitro characteristic malignant transformation. A well‐studied model estrogen‐dependent tumors is the MCF‐7 cell line, derived from a pleural metastasis human breast adenocarcinoma. We report cultivation cells, using routine methods, results extensive estrogen‐stimulated postconfluent accumulation characterized by discrete three‐dimensional arrays. Side view Nomarski optical sections revealed these to be...

An 8-mer peptide (EMTOVNOG) derived from α-fetoprotein was compared with tamoxifen for activity against growth of human breast cancer xenografts implanted in immune-deficient mice. Both and prevented estrogen-receptor-positive MCF-7 T47D xenografts. A subline MCF-7, made resistant to by a 6-month exposure this drug culture, found be vivo . Peptide completely the xenograft tamoxifen-resistant MCF-7. Neither nor effective slowing estrogen-receptor-negative MDA-MB-231 cancer. worrisome side...

Abstract: The peptide, EMTPVNPG, derived from alpha‐fetoprotein, inhibits estrogen‐stimulated growth of immature mouse uterus and estrogen‐dependent proliferation human breast cancer cells. However, the biological activities peptide diminish over time in storage, even when lyophilized state, probably because aggregation through hydrophobic interaction among monomers. Two analogs EMTPVNPG were designed with intent minimizing retaining activity during prolonged storage. EMTOVNOG, where O is...

Breast cancer is the most common among women, and tamoxifen preferred drug for estrogen receptor-positive breast treatment. Many of these cancers are intrinsically resistant to or acquire resistance during Consequently, there an ongoing need drugs that have different molecular targets. Previous work has shown 8-mer cyclic 9-mer peptides inhibit in mouse rat models, interacting with unsolved receptor, while smaller than eight amino acids did not. We show use replica exchange dynamics predicts...

Abstract Purpose: α-Fetoprotein (AFP) is a protein of pregnancy associated with decrease in lifetime risk breast cancer parous women. A synthetic, cyclic nonapeptide has been developed that mimics the antioncogenic active site AFP. To test hypothesis AFP-derived peptide (AFPep) can prevent cancer, N-methyl-N-nitrosourea–induced model was used rats. Experimental Design: AFPep given daily by injection beginning 10 days after N-methyl-N-nitrosourea treatment and continued for 23 (a time...

The AhR was initially identified as a ligand-activated transcription factor mediating effects of chlorinated dioxins and polycyclic aromatic hydrocarbons on cytochrome P450 1 (CYP1) expression. Recently, evidence supporting involvement the in cell-cycle regulation tumorigenesis has been presented. To further define roles cancer, we investigated expression cell proliferation, migration, invasion, MCF-7 human breast cancer cells. In these studies, properties cells were compared with those two...

Abstract: A 34‐amino acid synthetic peptide was derived from the third domain of human alpha‐fetoprotein, and shown to inhibit estrogen‐stimulated growth. Under certain conditions, however, lost growth‐inhibitory activity. biophysical study undertaken with a goal obtaining completely reliable preparations. The studied using gel‐filtration column chromatography as function concentration age solution, found exhibit complex aggregation behaviors. During early period (0–3 h) after dissolving...

Abstract: A synthetic peptide that inhibits the growth of estrogen receptor positive (ER+) human breast cancers, growing as xenografts in mice, has been reported. The cyclic 9‐mer peptide, cyclo [EMTOVNOGQ], is derived from α ‐fetoprotein (AFP), a safe, naturally occurring protein produced during pregnancy, which itself anti‐estrogenic and anti‐breast cancer activity. To determine pharmacophore series analogs was prepared using solid‐phase synthesis. Analogs were screened 1‐day bioassay,...

Abstract Cyclo [EKTOVNOGN] (AFPep), a cyclic 9‐amino acid peptide derived from the active site of alpha‐fetoprotein, has been shown to prevent carcinogen‐induced mammary cancer in rats and inhibit growth ER + human breast xenografts mice. Recently, studies using replica exchange molecular dynamics predicted that TOVN region AFPep might form dynamically stable putative Type I beta‐turn, thus be biologically without additional amino acids. The presented this paper were performed determine...