A5019563739- Prostate Cancer Treatment and Research
- Hormonal and reproductive studies
- Estrogen and related hormone effects
- RNA Interference and Gene Delivery
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Glutathione Transferases and Polymorphisms
- Ubiquitin and proteasome pathways
- Genital Health and Disease
- Bladder and Urothelial Cancer Treatments
- Pelvic floor disorders treatments
- Urological Disorders and Treatments
- Nuclear Receptors and Signaling
- Advanced biosensing and bioanalysis techniques
- Cancer, Lipids, and Metabolism
- Cytokine Signaling Pathways and Interactions
- Meta-analysis and systematic reviews
- Urinary Bladder and Prostate Research
- Genomics, phytochemicals, and oxidative stress
- Retinoids in leukemia and cellular processes
- Silymarin and Mushroom Poisoning
- Protein Degradation and Inhibitors
- Radiopharmaceutical Chemistry and Applications
- Sexual function and dysfunction studies
- Organic Light-Emitting Diodes Research
Taipei Medical University
2002-2024
Taipei Medical University Hospital
2009-2021
Institute of Biomedical Sciences, Academia Sinica
2012-2017
University of Rochester Medical Center
2003-2016
National Yang Ming Chiao Tung University
2010-2012
National Taiwan University
2012
National Defense Medical Center
2012
Academia Sinica
2012
Institute of Biological Chemistry, Academia Sinica
2010
National Tsing Hua University
2010
By using a cre-lox conditional knockout strategy, we report here the generation of androgen receptor (ARKO) mice. Phenotype analysis shows that ARKO male mice have female-like appearance and body weight. Their testes are 80% smaller serum testosterone concentrations lower than in wild-type (wt) Spermatogenesis is arrested at pachytene spermatocytes. The number size adipocytes also different between wt Cancellous bone volumes reduced compared with littermates. In addition, found average pups...
MicroRNAs (miRNA) mediate distinct gene regulatory pathways triggered by epidermal growth factor receptor (EGFR) activation, which occurs commonly in lung cancers with poor prognosis. In this study, we report the discovery and mechanistic characterization of miRNA miR-7 as an oncogenic "oncomiR" its role a key mediator EGFR signaling cancer cells. activation or ectopic expression Ras well c-Myc stimulated extracellular signal-regulated kinase (ERK)-dependent manner, suggesting that induces...
Using a Cre-Lox conditional knockout strategy, we generated germ cell-specific androgen receptor (AR) mouse (G-AR −/y ) with normal spermatogenesis. Sperm count and motility in epididymis from AR mice are similar to that of WT +/y mice. Furthermore, fertility tests show there was no difference fertility, almost 100% female pups sired by G-AR males younger than 15 weeks carried the deleted allele, suggesting efficient occurred cells during meiosis. Together, these data provide vivo evidence...
Despite androgen deprivation therapy (ADT) suppression of prostate cancer (PCa) growth, its overall effects on PCa metastasis remain unclear. Using human (C4-2B/THP1) and mouse (TRAMP-C1/RAW264.7) cells–macrophages co-culture systems, we found currently used anti-androgens, MDV3100 (enzalutamide) or Casodex (bicalutamide), promoted macrophage migration to cells that consequently led enhanced cell invasion. In contrast, the AR degradation enhancer, ASC-J9, suppressed both subsequent Mechanism...
Arginine plays diverse roles in cellular physiology. As a semi-essential amino acid, arginine deprivation has been used to target cancers with synthesis deficiency. Arginine-deprived cancer cells exhibit mitochondrial dysfunction, transcriptional reprogramming and eventual cell death. In this study, we show prostate that acts as an epigenetic regulator modulate histone acetylation, leading global upregulation of nuclear-encoded oxidative phosphorylation (OXPHOS) genes. TEAD4 is retained the...
Inhibition of invasion and metastasis has become a new approach for treatment advanced prostate cancer in which secondary hormone therapy failed. Accumulating evidence indicates that 1alpha,25-dihydroxyvitamin D3 (1,25-VD) suppresses progression by inhibition tumor growth metastasis. However, the detailed mechanisms underlying these effects remain to be determined. Here, we used vitro cell assay demonstrate 1,25-VD inhibits invasive ability human lines, LNCaP, PC-3 DU 145. Three major groups...
Androgen receptor (AR)-associated coregulator 70 (ARA70) was the first identified AR coregulator. However, its molecular mechanism and biological relevance to prostate cancer remain unclear. Here we show that ARA70 interacts with promotes activity via consensus FXXLF motif within ARA70-N2 domain (amino acids 176-401). it does not promote classic LXXLL located at amino 92-96, although this is important for interact other receptors, such as PPARγ. The mechanisms by which enhances...
In this nationwide population based study we used 5-year data on urinary calculi patient visits to emergency departments in Taiwan investigate the seasonal variation attacks and association with 5 climatic parameters.Comprehensive details total admissions were obtained from National Health Insurance Research Database (1999 2003), providing monthly attack rates per 100,000 of population. Subgroups incidences created gender 3 age groups (18 44, 45 64 65 years old or older). Following...
The successful translation of cytotoxic T lymphocyte antigen-4 (CTLA-4) blockade has revolutionized the concept cancer immunotherapy. Although monoclonal antibody therapeutics remain mainstream in clinical practice, aptamers are synthetic oligonucleotides that encompass antibody-mimicking functions. Here, we report a novel high-affinity CTLA-4-antagonizing DNA aptamer (dissociation constant, 11.84 nM), aptCTLA-4, which was identified by cell-based SELEX and high-throughput sequencing....
Rationale: One of the most common metabolic defects in cancers is deficiency arginine synthesis, which has been exploited therapeutically. Yet, challenges remain, and mechanisms arginine-starvation induced killing are largely unclear. Here, we sought to demonstrate underlying by starvation-induced cell death develop a dietary arginine-restriction xenograft model study vivo effects. Methods: Multiple castration-resistant prostate cancer lines were treated with starvation followed...
Low-back pain caused by intervertebral disc degeneration could be recovered the regeneration of nucleus pulposus (NP). This study aimed to establish a chondrogenic recovery model with promising human NP (hNP) cell line, an immortalized hNP (ihNP), which screening platform identify regenerative drugs. The ihNP cells were created from primary transfected retroviral vector-driven HPV16 E6/E7. Growth properties and characteristics evaluated comparing parental cells. Successful immortalization...
Cantharidin, an active component in mylabris, is used traditional Chinese medicine (TCM) to treat scabies and hepatoma, but accompanied by hemorrhagic cystitis. Evidence shows that cantharidin induces human bladder carcinoma cell death through COX-2 overexpression vitro. In TCM, Scutellaria baicalensis usually cure mylabris-induced hematuria. This work was undertaken determine the mechanisms of cantharidin-induced rat cystitis explore uroprotective effect S. baicalensis. vitro results showed...
Testicular germ cell tumors (TGCT) generally respond well to chemotherapy, but that express low levels of the transcription factor OCT4 are associated with chemoresistance and poor prognosis. Hypoxia is known induce drug resistance in TGCTs; however, mechanistic basis for reduced expression unclear. Here we show hypoxia reduces increases embryonal carcinoma (EC) cells cisplatin bleomycin. Furthermore, loss under can be triggered by sumoylation, which was regulated SUMO1 peptidase SENP1....
Apolipoprotein E (apoE) is synthesized in many tissues, and the liver primary site from which apoE redistributes cholesterol other lipids to peripheral tissues. Here we demonstrate that TR4 orphan nuclear receptor (TR4) can induce expression HepG2 cells. This TR4-mediated regulation of gene was further confirmed vivo using knockout mice. Both serum protein mRNA levels were significantly reduced Gel shift luciferase reporter assays demonstrated via a response element located hepatic control...
Growth arrest-specific (Gas) genes are expressed during serum starvation or contact inhibition of cells grown in culture. Here we report the isolation and characterization Gas8, a novel gene identified on basis its growth expression murine fibroblasts. We show that production Gas8 mRNA protein occurs adult mice predominantly testes, where is regulated postmeiotic development male gametocytes. Whereas low level was detected by Northern blotting testes neonates young adolescents, increased...
Abstract The testicular receptor 4 (TR4) is a member of the nuclear superfamily that controls various biological activities. A protective role TR4 against oxidative stress has recently been discovered. We here examined ionizing radiation (IR) and found small hairpin RNA mediated knockdown cells were highly sensitive to IR-induced cell death. IR exposure increased expression in scramble control expressing but not cells. Examination IR-responsive molecules Gadd45a, growth arrest DNA damage...