A5029745545- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Vibrio bacteria research studies
- Hepatitis Viruses Studies and Epidemiology
- Malaria Research and Control
- Vector-borne infectious diseases
- Salmonella and Campylobacter epidemiology
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- COVID-19 epidemiological studies
- SARS-CoV-2 detection and testing
- Dengue and Mosquito Control Research
- Viral Infections and Outbreaks Research
- Congenital Anomalies and Fetal Surgery
- Genetically Modified Organisms Research
- Bacillus and Francisella bacterial research
- Escherichia coli research studies
Taipei Medical University
2009-2024
Ministry of Health and Welfare
2004-2015
Development Center for Biotechnology
2004-2015
Childrens Heart Foundation
2015
National Health Research Institutes
2004-2014
Center for Disease Control
2004-2014
Animal Health Research Institute
2014
National Defence Medical Centre
2014
Centers for Disease Control and Prevention
2012-2013
Centers for Disease Control and Prevention
2007-2011
Influenza A viruses are major human and animal pathogens with huge economic societal impact from illness, hospitalizations, deaths. Virus-like particles (VLPs) of influenza virus have been suggested as a vaccine candidate offering improved safety efficacy. To develop this concept further, we established flexible platform to efficiently generate different subtypes mammalian-expressed VLPs. Here demonstrate that these mammalian VLPs strongly resemble the authentic in structure, particle size...
BackgroundPatients contracting influenza A(H7N9) infection often developed severe disease causing respiratory failure. Neuraminidase (NA) inhibitors (NAIs) are the primary option for treatment, but information on drug-resistance markers is limited.
Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase-polymerase chain reaction (RT-PCR), and serologic have been quickly developed. In this study, we evaluated four tests ( neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent [IFA], immunochromatographic test [ICT]) for detecting...
New variants of the influenza A(H1N1)pdm09 and A(H3N2) viruses were detected in Taiwan between 2012 2013. Some these not clinical specimens using a common real-time reverse transcription-PCR (RT-PCR) assay that targeted conserved regions viral matrix (M) genes. An analysis M gene sequences new revealed several newly emerging mutations located where primers or probes RT-PCR bind; included three (G225A, T228C, G238A) virus, as well one mutation (C163T) virus. These accumulated mismatch...
We used the dengue virus NS1 antigen (Ag) rapid test for on-site detection of imported cases at airports. Among 22 positive identified from 850 patients with a fever suspected to have dengue, 17 were Ag positive. These findings demonstrate usefulness in screening
The recent threats of influenza epidemics and pandemics have prioritized the development a universal vaccine that offers protection against wider variety infections. Here, we demonstrate genetically modified virus-like particle (VLP) vaccine, referred to as H5M2eN1-VLP, increased antigenic content NA induced rapid recall antibody HA2 after viral infection. As result, H5M2eN1-VLP vaccination elicited broad humoral immune response multiple proteins caused significant homologous RG-14 (H5N1)...
Background: Bacterial resistance remains a significant challenge, necessitating the development of new antibacterial strategies. This study introduces rapidly synthesized L-histidine- Fe3O4 (L-His-Fe3O4) nanozyme with enhanced peroxidase (POD)-like activity, designed to improve efficacy and accelerate healing bacteria-infected wounds. Methods: We successfully L-His-Fe3O4 using an ultrafast, room-temperature synthesis method, observed its anti-infection effect explored mechanism through in...
Two rotavirus (RV) vaccines (Rotarix and RotaTeq) are available on the private market in Taiwan, but not recommended for routine use. We examined RV vaccine effectiveness (VE) against severe acute gastroenteritis (AGE) among Taiwanese infants to inform policymakers potential benefits of national introduction.From May 2009 April 2011, a case-control assessment VE AGE was conducted at 3 hospital-based surveillance sites Taiwan. Case-patients included children aged 8-35 months, hospitalized...
The global epidemiological landscape of childhood acute gastroenteritis (AGE) is changing after the introduction 2 effective rotavirus vaccines in 2006. A comprehensive evaluation for viral etiology AGE Taiwan, where vaccination was provided by private sector since 2006, lacking. From 2009 to 2011, children younger than 5 years age with who were hospitalized at 3 sentinel hospitals enrolled this surveillance study. Stool specimens tested rotavirus, norovirus, enteric adenovirus, and...
Abstract Background Human enterovirus 71 (EV-71) is known of having caused numerous outbreaks hand-foot-mouth disease, and other clinical manifestations globally. In 2008, 989 EV-71 strains were isolated in Taiwan. Results this study, the genetic antigenic properties these analyzed diversity subgenogroups surfacing Taiwan was depicted, which includes 3 previously reported C5, B5, C4, one C2-like subgenogroup. Based on phylogenetic analyses using their complete genome nucleotide sequences...
We carried out virological surveillance of dengue virus (DENV) in field-caught Aedes mosquitoes during 2004–2007 to estimate the monthly prevalence infected females high-risk areas Taiwan. A total 92,892 aegypti (43,133 and 49,759 males) 79,315 albopictus (57,319 21,996 adults were collected, grouped into 25,654 pools, processed for detection using a one-step SYBR Green–based real-time reverse transcriptase–polymerase chain reaction assay. DENVs periodically sympatrically detected Ae....
A dramatic increase in the frequency of H275Y mutation neuraminidase (NA), conferring resistance to oseltamivir, has been detected human seasonal influenza A/H1N1 viruses since season 2007-2008. The resistant emerged ratio 14.3% and quickly reached 100% Taiwan from September December 2008. To explore mechanisms responsible for emergence spread viruses, we analyzed complete genome sequences 25 collected during 2005-2009 Taiwan, which were chosen various clade 1, 2A, 2B-1, 2B-2, 2C-1 2C-2 by...
The annual recurrence of the influenza epidemic is considered to be primarily associated with immune escape due changes virus. In 2011–2012, B in Taiwan was unusually large, and predominant for a long time. To investigate genetic dynamics viruses during 2011–2012 epidemic, we analyzed sequences 4,386 collected from 2004 2012. data provided detailed insight into flux patterns multiple genotypes. We found that re-emergent TW08-I virus, which major genotype had co-circulated two others, TW08-II...
As an immunofluorescence assay for enterovirus D68 (EV-D68) is not available in the enteroviruses surveillance network Taiwan, EV-D68 may be actual pathogen of untypeable enterovirus-suspected isolates.The isolates collected from 2007 through 2014 were identified by nucleic acid amplification-based methods and sequencing VP1 region to analyze phylogeny epidemiology Taiwan.Twenty-nine sequenced, including 15 Cluster 3 14 1 viruses. Approximately 41% patients children under 5 years age their...
In Taiwan, avian influenza virus (AIV) subtypes H5N2, H6N1 and H7N3 have been identified in domestic poultry, several strains of these become endemic poultry. To evaluate the potential avian-to-human transmission due to occupational exposure, an exploratory analysis AIV antibody status poultry workers was conducted. We enrolled 670 workers, including 335 live vendors (LPVs), farmers (PFs), 577 non-poultry (NPWs). Serum titres against various viruses were analysed compared. The overall...
ABSTRACT A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe RT-PCR A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for simultaneous typing subtyping of viruses.
This study aimed to examine the epidemiological trends in dengue infection and impact of imported cases airport fever screening on community transmission Taiwan, a non-endemic island.All case data were obtained from surveillance system Taiwan Center for Disease Control analyzed by Pearson correlations, linear regression, geographical information (GIS)-based mapping. The implementing was evaluated using Student's t-test two-way analysis variance.A total 10 351 cases, including 7.1%...
Six persons in Taiwan who had contact with poultry infected influenza A(H5N2) showed seroconversion for the virus by hemagglutinin inhibition or microneutralization testing. We developed an ELISA based on nonstructural protein 1 of to differentiate natural infection from cross-reactivity after vaccination; 2 also this test.
We describe the development and evaluation of an indirect immunofluorescence assay (IFA) kit for rapid sensitive detection coxsackievirus A2, -4, -5, -6, -10. This IFA was determined to have 95.9 100% sensitivity 95.8 97.2% specificity. It also proved be beneficial in reducing number enteroviruses that are untypeable clinical virology laboratory.