A5005254109- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Hepatitis Viruses Studies and Epidemiology
- Respiratory viral infections research
- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Influenza Virus Research Studies
- Infection Control and Ventilation
- Clostridium difficile and Clostridium perfringens research
- Viral Infections and Vectors
- Food Safety and Hygiene
- Congenital Anomalies and Fetal Surgery
- Veterinary medicine and infectious diseases
- Virus-based gene therapy research
- Child Nutrition and Water Access
- Molecular Biology Techniques and Applications
- Infectious Encephalopathies and Encephalitis
- Animal Disease Management and Epidemiology
- Advanced Thermodynamic Systems and Engines
- Food Security and Health in Diverse Populations
- Bacterial Identification and Susceptibility Testing
- Bacterial Infections and Vaccines
- COVID-19 epidemiological studies
- Energy and Environmental Systems
Development Center for Biotechnology
2011-2023
Center for Disease Control
2005-2022
Changhua Christian Hospital
2022
Ministry of Health and Welfare
2005-2022
Chang Gung University
2017
Centers for Disease Control and Prevention
2006-2017
Linkou Chang Gung Memorial Hospital
2017
Centers for Disease Control and Prevention
2012-2015
Childrens Heart Foundation
2015
National Yang Ming Chiao Tung University
2011-2012
Abstract Sapovirus (SaV) is an agent of gastroenteritis for humans and swine, divided into five distinct genogroups (GI–GV) based on its capsid gene sequences. Typical methods SaV detection include electron microscopy (EM), enzyme‐linked immunosorbent assay (ELISA), reverse transcription‐polymerase chain reaction (RT‐PCR). A novel TaqMan‐based real‐time RT‐PCR was developed that sensitive has the ability to detect broad range genetically diverse human strains. nucleotide alignment 10...
The P[19] genotype belongs to the P[II] genogroup of group A rotaviruses (RVs). However, unlike other RVs, which mainly infect humans, RVs commonly animals (pigs), making unique for study RV diversity and host ranges. Through in vitro binding assays saturation transfer difference (STD) nuclear magnetic resonance (NMR), we found that could bind mucin cores 2, 4, 6, as well type 1 histo-blood antigens (HBGAs). common sequences these glycans serve minimal units, while additional residues, such...
In Taiwan, acute gastroenteritis outbreaks caused by a new norovirus genotype GII.2 increased sharply toward the end of 2016. Unlike previous outbreaks, which often involved restaurants, mainly occurred in schools. Phylogenetic analysis indicates that these noroviruses are recombinant GII.P16-GII.2 strains.
Two rotavirus (RV) vaccines (Rotarix and RotaTeq) are available on the private market in Taiwan, but not recommended for routine use. We examined RV vaccine effectiveness (VE) against severe acute gastroenteritis (AGE) among Taiwanese infants to inform policymakers potential benefits of national introduction.From May 2009 April 2011, a case-control assessment VE AGE was conducted at 3 hospital-based surveillance sites Taiwan. Case-patients included children aged 8-35 months, hospitalized...
The global epidemiological landscape of childhood acute gastroenteritis (AGE) is changing after the introduction 2 effective rotavirus vaccines in 2006. A comprehensive evaluation for viral etiology AGE Taiwan, where vaccination was provided by private sector since 2006, lacking. From 2009 to 2011, children younger than 5 years age with who were hospitalized at 3 sentinel hospitals enrolled this surveillance study. Stool specimens tested rotavirus, norovirus, enteric adenovirus, and...
Acute gastroenteritis (AGE) can be caused by a wide array of pathogens, including bacteria, viruses, and parasites. A prospective study to investigate the epidemiology clinical presentation in young children hospitalized with AGE was conducted medical center southern Taiwan.Patients aged less than 5 years who due National Cheng Kung University Hospital were enrolled from July 2014 June 2016. The demographic information, features laboratory data collected chart reviews, stool samples sent...
Rotavirus A (RVA) are a group of diverse viruses causing acute gastroenteritis (AGE) in humans and animals. Zoonotic transmission is an important mechanism for rotavirus evolution strain diversity humans, but the extent pigs as major reservoir human infection not clear. We have surveyed 153 pig farms across Taiwan with total 4588 porcine stool samples from three age groups 2014 to 2017. Nursing piglets (less than one month age) had higher detection rate older groups. Five VP7 (G) genotypes 5...
Abstract Background Influenza A virus infections occur in different species, causing mild-to-severe symptoms that lead to a heavy disease burden. H1N1, H1N2 and H3N2 are major subtypes of swine influenza viruses pigs occasionally infect humans. Methods case infected by novel was found through laboratory surveillance system for viruses. Clinical specimens were tested culture and/or real-time RT–PCR. The identified characterized gene sequencing phylogenetic analysis. Results In 2021, the first...
Campylobacteriosis is a common cause of bacterial gastroenteritis worldwide. This study aimed to investigate the potential risk factors, clinical and laboratory manifestations children with campylobacteriosis under five years old in Taiwan.This retrospective case-control was conducted ten major hospitals Taiwan from 2014 2017. Laboratory tests stool specimen were collected analyzed together questionnaire survey. Multivariate stepwise logistic regression model used for identification...
Abstract We previously reported the detection of genotype P[19] rotavirus strains from children hospitalized with acute dehydrating diarrhea during a 5‐year surveillance period in Taiwan. The characterization five (0.4% all typed), including three G3P[19], novel G5P[19], and unique G9P[19] is described this study. Phylogenetic analysis VP4, VP7, VP6, NSP4 genes was performed, which demonstrated lineages for respective genotypes VP4 VP7 genes. sequence similarities gene among Taiwanese human...
Rotavirus G3P[3] strains have been reported from a variety of species including humans, cats, dogs, monkeys, goats, and cows. Here, we report the characterization first human rotavirus East Asia identified in 2-year-old child who was treated hospital's emergency ward Taiwan February 2005. Sequence phylogenetic analysis demonstrated close genetic relationship between VP4, VP6, VP7, NSP4 genes Taiwanese strain 04-94s51 an Italian canine isolated decade ago, suggesting origin for strain. In...
Norovirus is a common acute gastroenteritis (AGE) pathogen across all age groups worldwide, which difficult to differentiate from other pathogens. This study aimed understand the clinical characteristics and risk factors of norovirus among children in Taiwan. A prospective AGE surveillance was conducted aged ≤5 years who were hospitalized 10 major hospitals Taiwan between 2014 2017. The non-AGE control group included healthy matched based on age, gender, season, geographic area. Overall, 674...
Despite the increasingly recognized role of norovirus in global acute gastroenteritis (AGE), specific estimates associated disease burden remain sparse, primarily due to limited availability sensitive diagnostics clinical setting. We sought estimate incidence norovirus-associated hospitalizations by age group Taiwan using a previously developed indirect regression method.AGE-related were identified International Classification Diseases, Ninth Revision, Clinical Modification codes abstracted...
Enterotoxigenic Bacteroides fragilis (ETBF) and toxin-encoding Clostridium difficile (TXCD) are associated with gastroenteritis. Routine anaerobic blood culture for recovery of these pathogens is not used the detection their toxins, especially toxin-variant TXCD. The aim this study was to investigate prevalence risk factors genotypes anaerobes in patients acute diarrheal illnesses.The data samples 513 gastroenteritis were collected a Taipei emergency department from March 1, 2006 December...
Sapoviruses are associated with acute gastroenteritis. Human sapoviruses classified into four distinct genogroups (GI, GII, GIV, and GV) based on their capsid gene sequences. A TaqMan probe‐based real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) assay that detects the representative strains of these is widely used for screening fecal specimens, shellfish, environmental water samples. However, since development this test, more genetically diverse sapovirus have been reported,...
The causative pathogen is rarely identified in the emergency department (ED), since results of cultures are usually unavailable. As a result, antimicrobial treatment may be overused. aim our study was to investigate pathogens, risk factors acute gastroenteritis, and predictors bacterial gastroenteritis ED.We conducted matched case-control 627 stool samples 612 pairs.Viruses (41.3%) were leading cause with noroviruses (32.2%) being most prevalent, followed by bacteria (26.8%) Giardia lamblia...
Noroviruses are an important aetiological agent of acute gastroenteritis. They responsible for large outbreaks disease in the community, hospitals and long-term-care facilities. The clinical manifestations norovirus psychiatric units rarely described. burden impact highlight importance timely notification investigation these outbreaks. We analysed characteristics four which occurred during a 3-year period in-patient care unit. A total 184 patients were affected included 172 hospitalized...