A5100351064- Glycosylation and Glycoproteins Research
- Influenza Virus Research Studies
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Proteoglycans and glycosaminoglycans research
- Galectins and Cancer Biology
- Viral Infections and Vectors
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Forensic and Genetic Research
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Genetic Mapping and Diversity in Plants and Animals
- Mycobacterium research and diagnosis
- Virology and Viral Diseases
- Fungal Infections and Studies
- Bacterial Infections and Vaccines
Imperial College London
2016-2025
Anhui Medical University
2016-2025
Shanxi Agricultural University
2025
Chengdu University
2025
First Affiliated Hospital of Anhui Medical University
2018-2025
Jiaozuo University
2025
Chengdu Women's and Children's Central Hospital
2023-2025
Shanghai Xuhui Central Hospital
2025
Fudan University
2025
Tianjin Medical University Cancer Institute and Hospital
2024-2025
Abstract Since 2002, beta coronaviruses (CoV) have caused three zoonotic outbreaks, SARS-CoV in 2002–2003, MERS-CoV 2012, and the newly emerged SARS-CoV-2 late 2019. However, little is currently known about biology of SARS-CoV-2. Here, using S protein pseudovirus system, we confirm that human angiotensin converting enzyme 2 (hACE2) receptor for SARS-CoV-2, find enters 293/hACE2 cells mainly through endocytosis, PIKfyve, TPC2, cathepsin L are critical entry, less stable than S. Polyclonal...
Broadly neutralizing HIV antibodies (bNAbs) can recognize carbohydrate-dependent epitopes on gp120. In contrast to previously characterized glycan-dependent bNAbs that high-mannose N -glycans, PGT121 binds complex-type -glycans in glycan microarrays. We isolated the B-cell clone encoding PGT121, which segregates into PGT121-like and 10-1074–like groups distinguished by sequence, binding affinity, carbohydrate recognition, activity. Group 10-1074 exhibits remarkable potency breadth but no...
The epithelial to mesenchymal transition (EMT) plays crucial roles in the formation of body plan and also tumor invasion process. In addition, EMT causes disruption cell-cell adherence, loss apico-basal polarity, matrix remodeling, increased motility invasiveness promoting metastasis. microenvironment an important role facilitating cancer metastasis may induce occurrence cells. A large number inflammatory cells infiltrating site, as well hypoxia existing a area tumor, addition many stem...
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a newly discovered bunyavirus, SFTS virus (SFTSV), and causes high fatality (12% on average as 30%). The objective of this study was to determine whether SFTSV could be transmitted from person person. We analyzed sera 13 patients two clusters unknown diseases that occurred between September November 2006 in Anhui Province China for antibody indirect immunofluorescence assay RNA RT-PCR. found all...
Abstract Gnetophytes are an enigmatic gymnosperm lineage comprising three genera, Gnetum , Welwitschia and Ephedra which morphologically distinct from all other seed plants. Their distinctiveness has triggered much debate as to their origin, evolution phylogenetic placement among To increase our understanding of the gnetophytes, relation plants, we report here a high-quality draft genome sequence for montanum first any gnetophyte. By using novel assembly strategy deal with high levels...
Exercise is an effective nonpharmacological strategy to alleviate diabetic cardiomyopathy (DCM) through poorly defined mechanisms. FGF21 (fibroblast growth factor 21), a peptide hormone with pleiotropic benefits on cardiometabolic homeostasis, has been identified as exercise responsive factor. This study aims investigate whether signaling mediates the of DCM, and if so, elucidate underlying The global or hepatocyte-specific knockout mice, cardiomyocyte-selective β-klotho (the obligatory...
Breakthrough infections by SARS-CoV-2 variants become the global challenge for pandemic control. Previously, we developed protein subunit vaccine ZF2001 based on dimeric receptor-binding domain (RBD) of prototype SARS-CoV-2. Here, a chimeric RBD-dimer approach to adapt variants. A prototype-Beta was first designed resistant Beta variant. Compared with its homotypic forms, elicited broader sera neutralization and conferred better protection in mice. The further verified macaques. This...
Understanding the driving forces and intrinsic mechanisms of microbial competition is a fundamental question in ecology. Despite well-established negative correlation between exploitation phylogenetic distance, process interference that exemplified by antagonism remains controversial. Here, we studied genus Bacillus, commonly recognized producer multifarious antibiotics, to explore role patterns biosynthetic gene clusters (BGCs) mediating relationship phylogeny. Comparative genomic analysis...
H5 influenza is considered a potential pandemic threat. Recently, viruses belonging to clade 2.3.4.4b have caused large outbreaks in avian and multiple nonhuman mammalian species. Previous studies identified molecular phenotypes of the viral hemagglutinin (HA) protein that contribute humans, including cell entry, receptor preference, HA stability, reduced neutralization by polyclonal sera. However, prior experimental work has only measured how these are affected handful >10,000 different...
Platelet-derived growth factor BB (PDGF-BB) has been shown to be an extremely potent negative regulator of smooth muscle cell (SMC) differentiation. Moreover, previous studies have demonstrated that the Kruppel-like transcription (KLF) 4 potently represses expression multiple SMC genes. However, mechanisms whereby KLF4 suppresses gene are not known, nor is it clear whether contributes PDGF-BB-induced down-regulation The goals present were determine molecular by which genes and suppression...
N-Glycosylation starts in the endoplasmic reticulum (ER) where a 14-sugar glycan composed of three glucoses, nine mannoses, and two N-acetylglucosamines (Glc(3)Man(9)GlcNAc(2)) is transferred to nascent proteins. The glucoses are sequentially trimmed by ER-resident glucosidases. Glc(3)Man(9)GlcNAc(2) moiety substrate for oligosaccharyltransferase; Glc(1)Man(9)GlcNAc(2) Man(9)GlcNAc(2) intermediates signals glycoprotein folding quality control calnexin/calreticulin cycle. Here, we report...
The role of conformation-based quality control in the early secretory pathway is to eliminate misfolded polypeptides and unassembled multimeric protein complexes from endoplasmic reticulum, ensuring deployment only functional molecules distal sites. intracellular fate terminally human α<sub>1</sub>-antitrypsin was examined hepatoma cells identify asparagine-linked oligosaccharide modification selection glycoproteins for degradation by cytosolic proteasome. Proteasomal required physical...
Mutations in the receptor-binding site of hemagglutinin pandemic influenza A(H1N1) 2009 viruses have been detected sporadically. An Asp222Gly (D222G) substitution has associated with severe or fatal disease. Here we show that 222G variants infected a higher proportion ciliated cells cultures human airway epithelium than did 222D 222E, which targeted mainly nonciliated cells. Carbohydrate microarray analyses showed bind broader range α2-3-linked sialyl receptor sequences type expressed on...
Anti-β-glucan antibodies elicited by a laminarin-conjugate vaccine confer cross-protection to mice challenged with major fungal pathogens such as Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans. To gain insights into protective β-glucan epitope(s) protection mechanisms, we studied two anti-β-glucan monoclonal (mAb) identical complementarity-determining regions but different isotypes (mAb 2G8, IgG2b mAb 1E12, IgM). C. the most relevant pathogen for humans, was used model....