A5000217483- Marine Ecology and Invasive Species
- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- Marine Biology and Environmental Chemistry
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Marine Invertebrate Physiology and Ecology
- Animal Genetics and Reproduction
- Physiological and biochemical adaptations
- Receptor Mechanisms and Signaling
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- Genetic and Kidney Cyst Diseases
- Neuroendocrine regulation and behavior
- RNA Research and Splicing
- MicroRNA in disease regulation
- Renal and related cancers
- Cephalopods and Marine Biology
- Zebrafish Biomedical Research Applications
- Hypothalamic control of reproductive hormones
- Neuroinflammation and Neurodegeneration Mechanisms
- Planarian Biology and Electrostimulation
Konan University
2015-2025
University of Hyogo
2004-2012
Montreal Neurological Institute and Hospital
2010
Hyogo University
2007
Japan Science and Technology Agency
2005
Himeji University
2002-2004
Hyogo Prefectural Institute of Technology
2001-2003
Hokkaido University
1996-2001
Pennsylvania State University
1999
Kyoto University
1991-1996
Steroid-resistant nephrotic syndrome (SRNS) is a frequent cause of end-stage renal failure. Identification single-gene causes SRNS has generated some insights into its pathogenesis; however, additional genes and disease mechanisms remain obscure, continues to be treatment refractory. Here we have identified 6 different mutations in coenzyme Q10 biosynthesis monooxygenase (COQ6) 13 individuals from 7 families by homozygosity mapping. Each mutation was linked early-onset with sensorineural...
We mapped 633 markers (488 AFLPs, 28 RAPDs, 34 IRSs, 75 ESTs, 4 STSs, and phenotypic markers) for the Medaka Oryzias latipes, a teleost fish of order Beloniformes. Linkage was determined using reference typing DNA panel from 39 cell lines derived backcross progeny. This provided unlimited accumulation mapping data. The total map length 1354.5 cM 24 linkage groups were detected, corresponding to haploid chromosome number organism. Thirteen 49 each group obtained. Conserved synteny between...
ANISEED (www.aniseed.cnrs.fr) is the main model organism database for tunicates, sister-group of vertebrates. This release gives access to annotated genomes, gene expression patterns, and anatomical descriptions nine ascidian species. It provides increased integration with external molecular taxonomy databases, better support epigenomics datasets, in particular RNA-seq, ChIP-seq SELEX-seq, features novel interactive interfaces existing datatypes. In particular, cross-species navigation...
Developmental biology aims to understand how the dynamics of embryonic shapes and organ functions are encoded in linear DNA molecules. Thanks recent progress genomics imaging technologies, systemic approaches now used parallel with small-scale studies establish links between genomic information phenotypes, often described at subcellular level. Current model organism databases, however, do not integrate heterogeneous data sets different scales into a global view developmental program. Here,...
The autosomal recessive kidney disease nephronophthisis (NPHP) constitutes the most frequent genetic cause of terminal renal failure in first 3 decades life. Ten causative genes (NPHP1-NPHP9 and NPHP11), whose products localize to primary cilia-centrosome complex, support unifying concept that cystic diseases are "ciliopathies". Using genome-wide homozygosity mapping, we report here what believe be a new locus (NPHP-like 1 [NPHPL1]) for an NPHP-like nephropathy. In 2 families with phenotype,...
Abstract Glutamate is a major neurotransmitter in the excitatory synapses of both vertebrate and invertebrate nervous systems involved many neural processes including photo‐, mechano‐, chemosensations, development, motor control, learning, memory. We identified characterized gene ( Ci ‐ VGLUT ) encoding member vesicular glutamate transporter subfamily, specific marker glutamatergic neurons, ascidian Ciona intestinalis . The expressed adhesive organ, epidermal brain vesicle, but not visceral...
The retina of craniates/vertebrates has been proposed to derive from a photoreceptor prosencephalic territory in ancestral chordates, but the evolutionary origin different cell types making is disputed. Except for photoreceptors, existence homologs retinal cells remains uncertain outside vertebrates.The expression genes expressed sensory vesicle ascidian Ciona intestinalis including those encoding components monoaminergic neurotransmission systems, was analyzed by situ hybridization or vivo...
A group of non-visual opsins specific to vertebrates is essential understand evolution lateral eyes, one the most prominent innovations in this lineage. Nevertheless, our knowledge their evolutionary history remains limited. To develop an integrated view evolution, we surveyed these (VA opsin, pinopsin, parapinopsin, parietopsin, and parapinopsin-like) 451 vertebrate genomes. Through extensive manual curation, completed a high-quality catalog. We could not find them 202 mammals, supporting...
A novel gene encoding visual pigment, Ci‐opsin1 , was identified in a primitive chordate, the ascidian, Ciona intestinalis . Molecular phylogenetic analysis and exon–intron organization suggest that is closely related to retinal pineal opsins of vertebrates. During embryogenesis, transcripts were first detected part brain mid tailbud embryos; its expression confined photoreceptor cells ocellus (eye spot) larval as development proceeded. These results common descent ascidian vertebrate eyes....
The lineage of muscle cells in the ascidian embryo is well documented. Of B-line blastomeres, B7.4 become restricted to give rise at 44-cell stage, whereas B7.8 and B7.5 64-cell stage. In this study we addressed three issues concerning timing initiation muscle-specific structural gene transcription: (i) whether transcription genes correlated that developmental fate restriction; (ii) if there a relationship timing, it applicable B7.4-, B7.5- B7.8-sublineages; (iii) two genes, one for actin...
Abstract The vertebrate‐type opsin, Ci‐opsin1, is localized in the outer segments of photoreceptor cells larvae ascidian Ciona intestinalis . absorption spectrum photopigment reconstituted from Ci‐opsin1 and 11‐ cis ‐retinal suggested that responsible for photic behavior larvae. structure function Ci‐opsin1‐positive were examined by immunohistochemistry, confocal microscopy, electron laser ablation, behavioral analysis. have three morphologically distinct groups brain vesicle. Group I group...
The ascidian larva is an excellent model for studies of the functional organization and neuronal circuits chordates due to its remarkably simple central nervous system (CNS), comprised about 100 neurons. To date, however, identities various neurons in larva, particularly their neurotransmitter phenotypes, are not well established. Acetylcholine, GABA, glycine critical neurotransmitters locomotion many animals. We visualized putative cholinergic GABAergic/glycinergic by immunofluorescent...
Gonadotropin-releasing hormone (GnRH) is a neuroendocrine peptide that plays central role in the vertebrate hypothalamo-pituitary axis. The roles of GnRH control reproductive functions have been established, while its non-reproductive function has suggested but less well understood. Here we show tunicate Ciona intestinalis larval stage prominent system spanning entire length nervous system. Tunicate receptors are phylogenetically closest to receptors, yet functional analysis revealed these...
We isolated 5' flanking regions of four genes, Ci-Galphai1, Ci-arr, Ci-vAChTP, and Ci-vGAT, each which is expressed in distinct sets neurons the central nervous system ascidian Ciona intestinalis, we examined their function by introducing green fluorescent protein (GFP)-fusion constructs into embryos. The reporter gene driven region Ci-vAChTP recapitulated endogenous expression patterns, while that Ci-vGAT can drive GFP particular subsets expressing gene. Deletion analysis revealed...
Gonadotropin-releasing hormones (GnRHs) play pivotal roles in control of reproduction via a hypothalamic–pituitary–periphery endocrine system and nervous systems not only vertebrates but also invertebrates. GnRHs trigger several signal transduction cascades GnRH receptors (GnRHRs), members the G protein–coupled receptor (GPCR) family. Recently, six (tunicate [tGnRH]-3 to tGnRH-8) four GnRHRs (Ciona intestinalis [Ci]-GnRHR1 GnRHR-4), including species-specific paralog, Ci-GnRHR4 (R4) regarded...