A5101836007- Animal Genetics and Reproduction
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Environmental Toxicology and Ecotoxicology
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Chromosomal and Genetic Variations
- bioluminescence and chemiluminescence research
- Genomics and Chromatin Dynamics
- Physiological and biochemical adaptations
- Genetic diversity and population structure
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Effects of Radiation Exposure
- Developmental Biology and Gene Regulation
- Plant Genetic and Mutation Studies
- Identification and Quantification in Food
- Photoreceptor and optogenetics research
- Hypothalamic control of reproductive hormones
- Light effects on plants
- Japanese History and Culture
- Skin Protection and Aging
The University of Tokyo
2015-2024
Tōyō Bunko
2020
Frontier Science Foundation
2017
Tokyo University of Science
1995-2010
Ube Frontier University
2006-2010
Kitasato University
2010
Toho University
2004
Hokkaido University
2003
Jikei University School of Medicine
1992
Kyoto University
1987-1989
Abstract TLR22 occurs exclusively in aquatic animals and its role is unknown. Herein we show that the fugu (Takifugu rubripes) (fg)TLR3 fgTLR22 link IFN-inducing pathway via fg Toll-IL-1R homology domain-containing adaptor protein 1(fgTICAM-1, or TRIF) fish cells. fgTLR3 resides endoplasmic reticulum recognizes relatively short-sized dsRNA, whereas long-sized dsRNA on cell surface. On poly(I:C)-stimulated cells, both recruit fgTICAM-1, which turn moves from TLR to a cytoplasmic signalosome...
We mapped 633 markers (488 AFLPs, 28 RAPDs, 34 IRSs, 75 ESTs, 4 STSs, and phenotypic markers) for the Medaka Oryzias latipes, a teleost fish of order Beloniformes. Linkage was determined using reference typing DNA panel from 39 cell lines derived backcross progeny. This provided unlimited accumulation mapping data. The total map length 1354.5 cM 24 linkage groups were detected, corresponding to haploid chromosome number organism. Thirteen 49 each group obtained. Conserved synteny between...
The mapping of Hox clusters and many duplicated genes in zebrafish indicated an extra whole-genome duplication ray-fined fish. However, to reconstruct the preduplication chromosomes (proto-chromosomes), comparative genomic studies more distantly related teleosts are essential. Medaka ideal for this purpose, because their lineages separated from last common ancestor approximately 140 million years ago. To ancient vertebrate chromosomes, including humans 450 ago, we mapped 818 expressed...
We previously performed mutant screens in the medaka for defects gonadal development and identified a of interest this regard, which was designated as hotei (hot). This manifests number remarkable phenotypic abnormalities including: (i) excessive proliferation germ cells that initiates at around hatching stage regardless genetic sex fish; (ii) initiation premature meiosis phenotypically male hot homozygotes; (iii) one-half hot-homozygous XY fish undergo reversal, accompanies expression...
ABSTRACT UV inactivation, photoreactivation, and dark repair of Escherichia coli Cryptosporidium parvum were investigated with the endonuclease sensitive site (ESS) assay, which can determine UV-induced pyrimidine dimers in genomic DNA microorganisms. In a 99.9% inactivation E. , high correlation was observed between dose irradiation number induced . The colony-forming ability also correlated highly DNA, indicating that ESS assay is comparable to method conventionally used measure ability....
In almost all eukaryotes, mitochondrial (mt) genes are transmitted to progeny mainly from the maternal parent. The most popular explanation for this phenomenon is simple dilution of paternal mtDNA, because gametes (sperm) much smaller than (egg) and contribute a limited amount mitochondria progeny. Recently, has been challenged in several reports that describe active digestion sperm down-regulation mtDNA replication sperm, proteolysis triggered by ubiquitination. investigation, we visualized...
Abstract We have established a reverse genetics approach for the routine generation of medaka ( Oryzias latipes ) gene knockouts. A cryopreserved library N -ethyl- -nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing induced point mutations. Nonsense and splice site mutations were retrieved Blm , Sirt1 Parkin p53 genes functional characterization mutants indicated complete knockout function. The current resource is expected to contain knockouts most genes.
Natriuretic peptides (NPs) are major cardiovascular and osmoregulatory hormones in vertebrates. Although tetrapods generally have three subtypes, atrial NP (ANP), B-type (BNP), C-type (CNP), some teleosts lack BNP, sharks hagfish only one NP. Thus, NPs diverged during fish evolution, possibly reflecting changes systems. In this study, we found, by cDNA cloning, four distinct CNPs (1 through 4) the medaka (Oryzias latipes) puffer (Takifugu rubripes), although to our knowledge no more than two...
Species-specific colors and patterns on animal body surfaces are determined primarily by neural-crest-derived pigment cells in the skin (chromatophores). However, even closely related species display widely differing patterns. These contrasting aspects of chromatophores (i.e., fixed developmental control within extreme diversity among species) seem to be a curious suitable subject for understanding evolution organisms. Here we identify gene responsible medaka "color interfere" mutants...
Mouse phospholipase C, zeta 1 (PLCZ1), a strong candidate of egg-activating sperm factor, induces Ca2+ oscillations and accumulates into formed pronucleus (PN) when expressed by cRNA injection. These activities were compared among mouse human PLCZ1, newly cloned rat Plcz1, medaka fish plcz1. The PLCZ1 proteins the four species have an approximately homologous sequence nuclear localization signal. However, translocation ability was defective in rat, human, eggs. Rat could not enter PN,...
Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to temporal expression, analysis function can also require spatial restriction. Recently, we reported a new method vivo , single‐cell using an infrared laser‐evoked operator (IR‐LEGO) system nematodes ( Caenorhabditis elegans ). It was demonstrated that (IR) irradiation could induce expression single cells without incurring cellular damage. Here, report application...
We report the identification and characterization of two distinct GnRH receptor (GnRH-R) subtypes, designated GnRH-R1 GnRH-R2, in a model teleost, medaka Oryzias latipes. These seven-transmembrane receptors contain cytoplasmic C-terminal tail, which has been found all other nonmammalian GnRH-Rs cloned to date. The gene is composed three exons separated by introns, whereas GnRH-R2 an additional intron therefore consists four introns. genes, both exist as single-copy genes genome, were mapped...
In the medaka, Oryzias latipes , sex is determined chromosomally. The chromosomes differ from those of mammals in that X and Y are highly homologous. Using backcross panels for linkage analysis, we mapped 21 sequence tagged site (STS) markers on (linkage group 1). genetic map chromosome was established using male female meioses. length shorter than region where recombination suppressed close to sex-determining gene y, while both telomeric regions. restriction does not occur uniformly...