A5103023350- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Insect and Arachnid Ecology and Behavior
- Silk-based biomaterials and applications
- Viral Infectious Diseases and Gene Expression in Insects
- Invertebrate Immune Response Mechanisms
- Physiological and biochemical adaptations
- Animal Behavior and Reproduction
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Fluid Dynamics and Mixing
- Parallel Computing and Optimization Techniques
- Silkworms and Sericulture Research
- Plant biochemistry and biosynthesis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Battery Materials and Technologies
- Plant and animal studies
- Biofuel production and bioconversion
- 3D Printing in Biomedical Research
- Synthesis and properties of polymers
- Antimicrobial Peptides and Activities
- Enzyme Catalysis and Immobilization
- Injection Molding Process and Properties
- Chemical Synthesis and Analysis
- Microbial Metabolic Engineering and Bioproduction
The University of Tokyo
2013-2022
Tokyo Institute of Technology
1995-2017
Isuzu Motors (United States)
2016
Kyushu University
2008-2014
University of Toyama
2007-2012
RIKEN Center for Computational Science
2011
University of California, San Francisco
2008
Kyoto Institute of Technology
2005-2007
Tokyo University of Agriculture
1988-2006
Osaka University
1990-2003
A peptide that strongly stimulates the secretion of juvenile hormone from corpora allata in vitro (allatotropin) has been purified extracts heads pharate adult Manduca sexta . The primary structure this 13-residue determined: H-Gly-Phe-Lys-Asn-Val-Glu-Met-Met-Thr-Ala-Arg-Gly-Phe-NH 2 This neurohormone no sequence similarity with any known neuropeptide other organisms. Synthetic allatotropin, as well truncation fragments, including one five amino terminal residues deleted, showed activity...
A peptide (Manduca sexta allatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified extracts of heads pharate M. a nine-step purification procedure. The primary structure this 15-residue determined: pGlu-Val-Arg-Phe-Arg-Gln-Cys- Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-OH, where pGlu is pyroglutamate). To our knowledge, neuro-hormone no sequence similarity with any known neuropeptide other organisms....
Ecdysteroids mediate a wide variety of developmental and physiological events in insects. In the postembryonic development insects, ecdysone is synthesized prothoracic gland (PG). Although many studies have revealed biochemical properties enzymes for ecdysteroid biosynthesis, most molecular identities these not been elucidated. Here we describe an uncharacterized cytochrome P450 gene, designated Cyp306a1, that essential biosynthesis PGs silkworm Bombyx mori fruit fly Drosophila melanogaster....
Three molecular forms of prothoracicotropic hormone were isolated from the head adult silkworm, Bombyx mori, and amino acid sequence 19 residues in terminus these hormones was determined. These exhibit significant homology with insulin insulin-like growth factors.
We have determined the complete amino acid sequence of 4K-PTTH-II, one three forms M r 4400 prothoracicotropic hormone silkworm Bombyx mori , active to brainless pupae Samia cynthia ricini . Like vertebrate insulin, it consists two nonidentical peptide chains (A and B chains). The A chain 20 residues. is a mixture four microheterogeneous peptides, which consist 28 residues, other two, 26 4K-PTTH-II has considerable homology (40%) with human resembles porcine relaxin both in carboxyl-terminal...
Prothoracicotropic hormone (PTTH), a brain secretory polypeptide of insects, stimulates the prothoracic glands to produce and release ecdysone, steroid essential insect development. The complementary DNAs encoding PTTH silkmoth Bombyx mori were cloned characterized, complete amino acid sequence was deduced. data indicated that is first synthesized as 224-amino precursor containing three proteolytic cleavage signals. carboxyl-terminal component (109 acids) follows last signal represents one...
Steroid hormones mediate a wide variety of developmental and physiological events in multicellular organisms. During larval pupal stages insects,the principal steroid hormone is ecdysone, which synthesized the prothoracic gland (PG) plays central role control development. Although many studies have revealed biochemical features ecdysone synthesis PG, aspects this pathway remained unclear at molecular level. We describe neverland (nvd) gene, encodes an oxygenase-like protein with Rieske...
Neuropeptides are an important class of molecules involved in diverse aspects metazoan development and homeostasis. Insects ideal model systems to investigate neuropeptide functions, the major focus insect research last decade has been on identification their receptors. Despite these vigorous efforts, receptors for some key neuropeptides such as prothoracicotropic hormone, eclosion hormone allatotropin (AT), remain undefined. In this paper, we report comprehensive cloning G protein-coupled...
A diuretic hormone (DH) has been isolated from pharate adult heads of Manduca sexta by a nine-step purification procedure. The primary structure the amino-terminal 40 residues was determined sequence analysis intact DH. an amidated carboxyl-terminal tryptic hexapeptide characterized peptide, and this later compared reversed-phase liquid chromatography with two synthetic hexapeptides free acid or amide at carboxyl terminus. complete M. DH established as 41-residue peptide without disulfide...
During development, the retinal vasculature grows toward hypoxic areas in an organized fashion. By contrast, ischemic retinopathies, new blood vessels grow out of surfaces without ameliorating hypoxia. Restoration proper angiogenic directionality would be great benefit to reoxygenize retina and resolve disease pathogenesis. Here, we show that binding semaphorin 3E (Sema3E) ligand transmembrane PlexinD1 receptor initiates a signaling pathway normalizes both developing retinas retinopathy. In...
Steroid hormones play essential roles in a wide variety of biological processes multicellular organisms. The principal steroid nematodes and arthropods are dafachronic acids ecdysteroids, respectively, both which synthesized from cholesterol as an indispensable precursor. first critical catalytic step the biosynthesis these ecdysozoan steroids is conversion to 7-dehydrocholesterol. However, enzymes responsible for 7,8-dehydrogenation remain unclear at molecular level. Here we report that...
Mechanisms for the uptake and transport of carotenoids, essential nutrients humans, are not well understood in any animal system. The Y ( Yellow blood ) gene, a critical cocoon color determinant silkworm Bombyx mori , controls carotenoids into intestinal mucosa silk gland. Here we provide evidence that gene corresponds to intracellular carotenoid-binding protein CBP gene. In recessive strain, absence an exon, likely due incorrect mRNA splicing caused by transposon-associated genomic...
Prothoracicotropic hormone (PTTH) stimulates ecdysteroid biosynthesis in the prothoracic gland (PG) of insects. A peptide inhibiting PG was isolated from extracts 2,000 larval brains silkworm, <i>Bombyx mori,</i> using a protocol that included four reversed-phase high performance liquid chromatography procedures. The primary structure this prothoracicostatic (Bom-PTSP) determined to be H-Ala-Trp-Gln-Asp-Leu-Asn-Ser-Ala-Trp-NH<sub>2</sub>. This neuropeptide has same sequence as Mas-MIP-I,...
One striking feature of dentate gyrus development, distinct from the other cortical structures, is relocation neural precursors ventricular zone to forming pole produce a lifelong neurogenic subgranular (SGZ). In this study, we demonstrate that progenitors first dwell for up 1 week in previously unrecognized intimately associated with pial meningeal surface lining outer edge dentate. This also serves as organizational matrix initial formation glial scaffolding. Timely clearance their...
Insect molting and metamorphosis are induced by steroid hormones named ecdysteroids, whose production is regulated various neuropeptides. We cloned the gene analyzed expression of prothoracicostatic peptide, a unique neuropeptide shown to suppress ecdysteroids in prothoracic gland silkworm, Bombyx mori . also characterized G protein-coupled receptor, which has previously been identified as an ortholog Drosophila sex peptide functional receptor. This receptor responded specifically peptides...