A5083794408- Silk-based biomaterials and applications
- Viral Infectious Diseases and Gene Expression in Insects
- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Silkworms and Sericulture Research
- Insect and Arachnid Ecology and Behavior
- Insect Utilization and Effects
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Insect Pheromone Research and Control
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Insect and Pesticide Research
- Virus-based gene therapy research
- Phytochemical compounds biological activities
- Transgenic Plants and Applications
- Entomopathogenic Microorganisms in Pest Control
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neural dynamics and brain function
- Reproductive Biology and Fertility
- Animal Behavior and Reproduction
- Histone Deacetylase Inhibitors Research
- Enzyme function and inhibition
- Polyamine Metabolism and Applications
Institute of Agrobiological Sciences
2014-2024
National Agriculture and Food Research Organization
2016-2024
The Dainippon Silk Foundation
2009-2016
University of Tsukuba
2010-2013
Tokyo University of Agriculture and Technology
2007
Hokkaido University
1973-2000
Bt toxins derived from the arthropod bacterial pathogen Bacillus thuringiensis are widely used for insect control as insecticides or in transgenic crops. resistance has been found field populations of several lepidopteran pests and laboratory strains selected with toxin. Widespread planting crops expressing raised concerns about potential increase mutations targeted insects. By using Bombyx mori a model, we identified candidate gene recessive form to Cry1Ab toxin on chromosome 15 by...
Insect molting and metamorphosis are intricately governed by two hormones, ecdysteroids juvenile hormones (JHs). JHs prevent precocious allow the larva to undergo multiple rounds of until it attains proper size for metamorphosis. In silkworm, Bombyx mori, several "moltinism" mutations have been identified that exhibit variations in number larval molts; however, none them characterized molecularly. Here we report identification characterization gene responsible dimolting (mod) mutant...
Mechanisms for the uptake and transport of carotenoids, essential nutrients humans, are not well understood in any animal system. The Y ( Yellow blood ) gene, a critical cocoon color determinant silkworm Bombyx mori , controls carotenoids into intestinal mucosa silk gland. Here we provide evidence that gene corresponds to intracellular carotenoid-binding protein CBP gene. In recessive strain, absence an exon, likely due incorrect mRNA splicing caused by transposon-associated genomic...
In insects and other animals, intraspecific communication between individuals of the opposite sex is mediated in part by chemical signals called pheromones. most moth species, male moths rely heavily on species-specific pheromones emitted female to identify orient towards an appropriate mating partner among a large number sympatric insect species. The silkmoth, Bombyx mori, utilizes simplest possible pheromone system, which single component, (E, Z)-10,12-hexadecadienol (bombykol), sufficient...
Silk of Bombyx mori can be used as various biomaterials. Especially, it is useful a protein for coating the surface cell culture plates since silk possesses biocompatibility to cultured cells. However, cell-adhesive ability weaker than collagen or fibronectin, which are plate more frequently (Yao et al. J. Biochem., 2004, 136, 643−649). To increase silk, we constructed transgenic silkworms, inserting modified fibroin light-chain genes making recombinant silks that possessed partial...
Abstract Silk is a protein fiber used to weave fabrics and as biomaterial in medical applications. Recently, genetically modified silks have been produced from transgenic silkworms. In the present study, silkworms for mass production of three colors fluorescent silks, (green, red, orange) are generated using vector originating fibroin H chain gene classical breeding method. The suitability recombinant making investigated by harvesting large amounts cocoons, obtained rearing over 20 thousand...
Engineered nucleases are artificial enzymes able to introduce double stranded breaks at desired genomic locations. The start the error-prone repair process of non-homologous end-joining (NHEJ), which eventually leads induction mutations target sites. We showed earlier that ZFNs and TALENs induce NHEJ in B. mori genome. In order optimize our mutagenesis protocol, we modified one reported truncated TALEN scaffolds optimized it for use embryo. also established a novel somatic cell assay...
Bombyx mori densovirus type 2 (BmDNV-2), a parvo-like virus, replicates only in midgut columnar cells and causes fatal disease. The resistance expressed some silkworm strains against the virus is determined by single gene, nsd-2, which characterized as nonsusceptibility irrespective of viral dose. However, responsible gene has been unknown. We isolated nsd-2 positional cloning. caused 6-kb deletion ORF encoding 12-pass transmembrane protein, member an amino acid transporter family, midgut....
Transgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due changes in color melanin pigments, which are widespread animals. Ubiquitous overexpression arylalkylamine-N-acetyl transferase silkworm, Bombyx mori, newly hatched first-instar larvae from black distinctive light brown color, and can be used as molecular by directly connecting baculovirus immediate early 1 gene promoter....
The white, scarlet and brown genes of Drosophila melanogaster encode three half-type ATP-binding cassette (ABC) transporters. In Drosophila, precursors ommochromes pteridines are transported by White/Scarlet White/Brown heterodimers, respectively. white egg 2 (w-2) mutant the silkworm, Bombyx mori, has eggs eyes because lack ommochrome granules in serosa eyes. Here, we report that silkworm w-2 locus encodes an ortholog scarlet. Our results indicate Scarlet forms a heterodimer with White to...
Dietary carotenoids are absorbed in the intestine and delivered to various tissues by circulating lipoproteins; however, mechanism underlying selective delivery of different carotenoid species individual remains elusive. The products Yellow cocoon (C) gene Flesh (F) silkworm Bombyx mori determine selectivity for transport lutein β-carotene, respectively, silk gland. We previously showed that C encodes Cameo2, a CD36 family member, which is thought function as transmembrane lipoprotein...
We constructed a new plasmid vector for the production of modified silk fibroin heavy chain protein (H-chain) in transgenic silkworm. The (pHC-null) contained promoter and 3' region gene encoding H-chain coding regions N-terminal domain C-terminal H-chain. For model protein, we cloned foreign that encoded EGFP between pHC-null generated silkworms produced H-chain, HC-EGFP. Transgenic HC-EGFP posterior part gland cells, secreted it into lumen gland, cocoon with as proteins. sequencing...
The larval integument of the silkworm, Bombyx mori, is opaque because urate granules accumulate in epidermis. Although biosynthetic pathway uric acid well studied, little known about how accumulates as epidermal cells. In distinct oily (od) mutant translucent inability to construct granules. Recently, we have found that od has a genomic deletion B. mori homologue human biogenesis lysosome-related organelles complex1, subunit 2 (BLOS2) gene (BmBLOS2). Here, performed molecular and functional...
Significance The olfactory system of male moths exhibits the ability to detect minute amounts sex pheromones. How this extreme sensitivity is achieved remains unclear. Using optogenetic techniques activate a pheromone-responsive receptor neuron, our results reveal that weak inputs, but not strong are temporally integrated in second-order projection neurons promote behavioral responsiveness. Furthermore, temporal integration inputs inhibited by GABAergic mechanisms, indicating GABA signaling...
Male moths use species-specific sex pheromones to identify and orientate toward conspecific females. Odorant receptors (ORs) for pheromone substances have been identified as in various moth species. However, direct vivo evidence linking the functional role of these ORs with behavioural responses is lacking. In silkmoth, Bombyx mori, female emit two components, bombykol bombykal, but only elicits sexual behaviour male moths. A receptor BmOR1 specifically tuned expressed specialized olfactory...
In Bombyx mori (B. mori), Fem piRNA originates from the W chromosome and is responsible for femaleness. The piRNA-PIWI complex targets cleaves mRNAs transcribed Masc gene. encodes a novel CCCH type zinc-finger protein required male-specific splicing of B. doublesex (Bmdsx) transcripts. present study, several silkworm strains carrying transgene, which piRNA-resistant mRNA (Masc-R), were generated. Forced expression Masc-R transgene caused female-specific lethality during larval stages. One...