A5038182471- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Plant Virus Research Studies
- Insect Resistance and Genetics
- Plant and animal studies
- Entomopathogenic Microorganisms in Pest Control
- Neurobiology and Insect Physiology Research
- Phytoplasmas and Hemiptera pathogens
- Insect Utilization and Effects
- Vector-borne infectious diseases
- Insect behavior and control techniques
- Invertebrate Immune Response Mechanisms
- Vector-Borne Animal Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Mosquito-borne diseases and control
- Hemiptera Insect Studies
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Silk-based biomaterials and applications
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Forest Insect Ecology and Management
- Animal Disease Management and Epidemiology
Robert Bosch (Germany)
2023
Institute of Agrobiological Sciences
2010-2021
Japan International Cooperation Agency
2017-2021
National Agriculture and Food Research Organization
1990-2020
The University of Tokyo
2009-2015
Kyushu Okinawa Agricultural Research Center
2013
Nihon University
2013
National Institute of Animal Health
2009
Institute of Vegetable and Floriculture Science
1990-2001
Chiba University
2001
The brown planthopper, Nilaparvata lugens, the most destructive pest of rice, is a typical monophagous herbivore that feeds exclusively on rice sap, which migrates over long distances. Outbreaks it have re-occurred approximately every three years in Asia. It has also been used as model system for ecological studies and developing effective management. To better understand how sap-sucking arthropod adapted to its exclusive host selection provide insights improve control, we analyzed genomes...
The presence, internal distribution, and phylogenetic position of endosymbiotic bacteria from four species specific-pathogen-free ticks were studied. These included the hard Ixodes scapularis (the black-legged tick), Rhipicephalus sanguineus brown dog Haemaphysalis longicornis African soft tick Ornithodoros moubata. PCR assays for bacteria, using two sets general primers eubacterial 16S 23S rRNA genes (rDNAs) seven specific wolbachial, rickettsial, or Francisella genes, indicated that I....
Bt toxins derived from the arthropod bacterial pathogen Bacillus thuringiensis are widely used for insect control as insecticides or in transgenic crops. resistance has been found field populations of several lepidopteran pests and laboratory strains selected with toxin. Widespread planting crops expressing raised concerns about potential increase mutations targeted insects. By using Bombyx mori a model, we identified candidate gene recessive form to Cry1Ab toxin on chromosome 15 by...
Bacillus thuringiensis is the most widely used biopesticide, and its Cry toxin genes are essential transgenes for generation of insect-resistant transgenic crops. Recent reports have suggested that ATP-binding cassette transporter subfamily C2 (ABCC2) proteins implicated in intoxication, a single amino acid insertion results high levels resistance to Cry1 toxins. However, there currently no available direct evidence functional interactions between ABCC2 To address this important knowledge...
Yeast-like endosymbionts (YLSs) of insects often are restricted to specific hosts and essential the host's survival. For example, in planthoppers (Homoptera: Delphacidae), function sterol utilization nitrogen recycling for hosts. Our study, designed investigate evolutionary changes YLS lineage involved planthopper association, strongly suggests an origin YLSs from within filamentous ascomycetes (Euascomycetes), not true yeasts (Saccharomycetes), as their morphology might indicate. During...
beta-Glucosidase [EC 3.2.1.21] and endo-beta-1,4-glucanase 3.2.1.4] activities were measured in the wood-eating higher termite Nasutitermes takasagoensis. activity was present mainly salivary glands (66.7%) midgut (22.2%), whereas detected (90.1%). Specific of also highest midgut, indicating that cellulose is digested midgut. The major component N. takasagoensis purified from whole termites by gel filtration on Sephaoryl S-200 HR, Superdex-75 hydroxyapatite column chromatography....
Wolbachia are bacterial endosymbionts in arthropods and filarial nematodes. They cause thelytoky, which is a form of parthenogenesis females produce without males, hymenopteran insects. Infection this parthenogenesis‐inducing has been restricted to the order Hymenoptera, but was found another insect order, Thysanoptera. A parthenogenetic colony predatory thrips Franklinothrips vespiformis (Aeolothripidae) possessed B-group Wolbachia. Male progeny were produced from by heat tetracycline...
Cardinium bacteria, members of the phylum Cytophaga-Flavobacterium-Bacteroides (CFB), are intracellular bacteria in arthropods that capable inducing reproductive abnormalities their hosts, which include parasitic wasps, mites, and spiders. A high frequency infection was detected planthoppers (27 out 57 species were infected). also found spider mites (9 22 Frequencies double by Wolbachia (Alphaproteobacteria manipulating reproduction hosts) disproportionately but not mites. new group...
Pigmentation patterning has long interested biologists, integrating topics in ecology, development, genetics, and physiology. Wild-type neonatal larvae of the silkworm, Bombyx mori , are completely black. By contrast, epidermis head homozygous recessive sex-linked chocolate ( sch ) mutant reddish brown. When incubated at 30 °C, mutants with allele fail to hatch; moreover, carrying lethal l do not hatch even room temperature (25 °C). positional cloning, we narrowed a region containing 239,622...
The establishment of a complete genomic sequence silkworm, the model species Lepidoptera, laid foundation for its functional genomics. A more annotation genome will benefit and comparative studies accelerate extensive industrial applications this insect. To realize these goals, we embarked upon large-scale full-length cDNA collection from 21 libraries derived 14 tissues domesticated silkworm performed full sequencing by primer walking 11,104 cDNAs. large average intron size was 1904 bp,...
Symbiotic bacteria are commonly associated with cells and tissues of diverse animals other organisms, which affect hosts' biology in a variety ways. Most these symbionts present the cytoplasm host maternally transmitted through generations. The paucity paternal symbiont transmission is likely relevant to extremely streamlined sperm structure: head consisting condensed nucleus tail made microtubule bundles, without symbiont-harboring that discarded process spermatogenesis. Here, we report...
Termites are among the most important cellulose-digesting animals on earth, and well-known for symbiotic relationship they have with cellulolytic trichomonad oxymonad flagellates (unicellular eukaryotes). Perhaps less is fact that approximately 75% of 2600 described termite species -- those belonging to family Termitidae do not harbour such flagellates. Unlike termites from other families, majority termitids consume wood, feeding instead soil, leaf litter, fungi, grass, or lichen. Recent...
In the blood (hemolymph) of silkworm Bombyx mori, insect cytokine paralytic peptide (PP) is converted from an inactive precursor to active form in response cell wall components microorganisms and contributes resistance infection. To investigate molecular mechanism underlying up-regulation host induced by PP, we performed oligonucleotide microarray analysis on RNA cells (hemocytes) fat body tissues larvae injected with PP. Expression levels a large number immune-related genes increased...
The brown planthopper (BPH), Nilaparvata lugens (Hemiptera, Delphacidae), is a serious insect pests of rice plants. Major means BPH control are application agricultural chemicals and cultivation resistant varieties. Nevertheless, strains that to have developed, appeared virulent against the Expressed sequence tag (EST) analysis related applications useful elucidate mechanisms resistance virulence reveal physiological aspects this non-model insect, with its poorly understood genetic...