A5064036671- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Utilization and Effects
- Silk-based biomaterials and applications
- Insect-Plant Interactions and Control
- Silkworms and Sericulture Research
- Invertebrate Immune Response Mechanisms
- Plant Virus Research Studies
- Animal Behavior and Reproduction
- Entomopathogenic Microorganisms in Pest Control
- Insect Pheromone Research and Control
- Insect and Pesticide Research
- Plant and animal studies
- Insects and Parasite Interactions
- Virus-based gene therapy research
- Studies on Chitinases and Chitosanases
- Insect behavior and control techniques
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Protein purification and stability
- Sphingolipid Metabolism and Signaling
- Physiological and biochemical adaptations
Kyoto University
2017-2024
The University of Tokyo
2006-2021
National Agriculture and Food Research Organization
2017-2020
Instituto Gulbenkian de Ciência
2020
Institute of Agrobiological Sciences
2010-2017
Tokyo University of Agriculture and Technology
2010
Tokyo University of Science
2008
Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting vectors containing homology arms and difficulties in inducing HR some cell type organisms represent technical hurdles for application of HR-mediated knock-in technology. Here, we introduce an alternative strategy transcription activator-like effector (TALENs) clustered regularly interspaced short palindromic repeats...
Current approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application to a great number species, especially those whose reproduction systems preclude access embryos injection. To overcome these limitations, we report simple and accessible method editing, termed "direct parental" CRISPR (DIPA-CRISPR). We show that injection Cas9 ribonucleoproteins (RNPs) haemocoel adult females efficiently introduces heritable mutations in...
Insect molting and metamorphosis are intricately governed by two hormones, ecdysteroids juvenile hormones (JHs). JHs prevent precocious allow the larva to undergo multiple rounds of until it attains proper size for metamorphosis. In silkworm, Bombyx mori, several "moltinism" mutations have been identified that exhibit variations in number larval molts; however, none them characterized molecularly. Here we report identification characterization gene responsible dimolting (mod) mutant...
Significance The juvenile–adult transition is a key developmental process in organisms. A long-held paradigm insect endocrinology that juvenile hormones (JHs) prevent metamorphosis until larvae attain an appropriate size for the transition. However, little known about roles JHs during embryonic and very early larval stages. We established knockouts of silkworm, classic model insect, show embryogenesis maintenance status stages are largely independent or JH-signaling pathway. Our results also...
Many larval color mutants have been obtained in the silkworm Bombyx mori. Mapping of melanin-synthesis genes on linkage map revealed that yellow and ebony were located near chocolate (ch) sooty (so) loci, respectively. In ch mutants, body neonate larvae markings elder instar are reddish brown instead normal black. Mutations at so locus produce smoky black pupae. F(2) analyses showed sequence polymorphisms perfectly cosegregated with mutant phenotypes, Both expressed epidermis during molting...
In the silkworm Bombyx mori , dietary flavonoids are metabolized and accumulate in cocoons, thereby causing green coloration. Classical genetic studies suggest that more than seven independent loci associated with this trait; however, because of complex inheritance pattern, none these have been characterized molecularly, a plausible comprehensive model for their action has not proposed. Here, we report identification gene responsible Green b ( Gb) locus involving cocoon trait. + Gb animals,...
The establishment of a complete genomic sequence silkworm, the model species Lepidoptera, laid foundation for its functional genomics. A more annotation genome will benefit and comparative studies accelerate extensive industrial applications this insect. To realize these goals, we embarked upon large-scale full-length cDNA collection from 21 libraries derived 14 tissues domesticated silkworm performed full sequencing by primer walking 11,104 cDNAs. large average intron size was 1904 bp,...
Engineered nucleases are artificial enzymes able to introduce double stranded breaks at desired genomic locations. The start the error-prone repair process of non-homologous end-joining (NHEJ), which eventually leads induction mutations target sites. We showed earlier that ZFNs and TALENs induce NHEJ in B. mori genome. In order optimize our mutagenesis protocol, we modified one reported truncated TALEN scaffolds optimized it for use embryo. also established a novel somatic cell assay...
Significance Sperm exhibit dramatic evolutionarily divergent morphologies in almost all taxa. Some sexually reproductive species show polymorphisms the sperm produced by single males. Here, we focused on Sex-lethal ( Sxl ), which is master sex-determination gene Drosophila melanogaster , and investigated its function lepidopteran insect Bombyx mori . Our genetic analyses revealed that essential for formation of anucleate nonfertile parasperm. It not expected would be involved polymorphisms....
In plants, many invading microbial pathogens are recognized by cell-surface pattern recognition receptors, which induce defense responses. Here, we show that the ceramide Phytophthora infestans-ceramide D (Pi-Cer D) from plant pathogenic oomycete P. infestans triggers responses in Arabidopsis. Pi-Cer is cleaved an Arabidopsis apoplastic ceramidase, NEUTRAL CERAMIDASE 2 (NCER2), and resulting 9-methyl-branched sphingoid base a plasma membrane lectin receptor-like kinase, RESISTANT TO...
The early embryo of the cockroach Blattella germanica exhibits high E93 expression. In general, triggers adult morphogenesis during postembryonic development. Here we show that is also crucial in embryogenesis cockroach, as a significant number E93-depleted embryos are unable to develop germ band under maternal RNAi treatment targeting . Moreover, transcriptomic analysis indicates depletion results important gene expression changes embryo, and many differentially expressed genes involved...
The white, scarlet and brown genes of Drosophila melanogaster encode three half-type ATP-binding cassette (ABC) transporters. In Drosophila, precursors ommochromes pteridines are transported by White/Scarlet White/Brown heterodimers, respectively. white egg 2 (w-2) mutant the silkworm, Bombyx mori, has eggs eyes because lack ommochrome granules in serosa eyes. Here, we report that silkworm w-2 locus encodes an ortholog scarlet. Our results indicate Scarlet forms a heterodimer with White to...
Abstract BACKGROUND The predatory flower bug Orius strigicollis serves as a valuable biocontrol agent against small arthropods; however, its effectiveness can vary, especially when population establishment fails due to low prey/pest densities. A promising approach improve the efficacy of O. is through gene editing. However, females lay their eggs in plant tissue, conventional embryo injection challenging this species. RESULTS In study, we aimed develop an efficient and practical editing...
ABSTRACT We identified a novel, 6,513-bp-long RNA, termed Bombyx mori macula-like latent virus (BmMLV) which abundantly expressed in B. cultured BmN cells. BmMLV RNA potentially encodes two proteins, putative replicase and coat protein, share structural features sequence similarities with those of plant virus, the genus Maculavirus . Northern blot analysis showed that transcripts were cells: 6.5-kb-long contains both protein genes, 1.2-kb-long only gene. Southern is not carried by genome....
The larval integument of the silkworm, Bombyx mori, is opaque because urate granules accumulate in epidermis. Although biosynthetic pathway uric acid well studied, little known about how accumulates as epidermal cells. In distinct oily (od) mutant translucent inability to construct granules. Recently, we have found that od has a genomic deletion B. mori homologue human biogenesis lysosome-related organelles complex1, subunit 2 (BLOS2) gene (BmBLOS2). Here, performed molecular and functional...