A5036834474- CRISPR and Genetic Engineering
- Microbial Natural Products and Biosynthesis
- Pancreatic function and diabetes
- Cancer therapeutics and mechanisms
- Developmental Biology and Gene Regulation
- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Colorectal Cancer Treatments and Studies
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Animal Genetics and Reproduction
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Skin and Cellular Biology Research
- Blood Coagulation and Thrombosis Mechanisms
- Cancer Treatment and Pharmacology
- Nausea and vomiting management
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Inflammatory mediators and NSAID effects
- Silk-based biomaterials and applications
- Advanced Drug Delivery Systems
- Enzyme Production and Characterization
National Institute for Basic Biology
2018-2025
Kin-ikyo Chuo Hospital
2025
Hiroshima University
2010-2024
Tokyo University of Pharmacy and Life Sciences
2024
Kao Corporation (Japan)
2024
Meiji University
2008-2023
National Institutes of Natural Sciences
2022-2023
Aichi University
2023
Tohoku University
1998-2022
Hoshi University
2020-2021
Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting vectors containing homology arms and difficulties in inducing HR some cell type organisms represent technical hurdles for application of HR-mediated knock-in technology. Here, we introduce an alternative strategy transcription activator-like effector (TALENs) clustered regularly interspaced short palindromic repeats...
Transcription activator-like effector (TALE) nuclease (TALEN) is a site-specific nuclease, which can be freely designed and easily constructed. Numerous methods of constructing TALENs harboring different TALE scaffolds repeat variants have recently been reported. However, the functionalities structurally not yet compared. Here, we report on functional differences among several types targeting same loci. Using HEK293T cell-based single-strand annealing Cel-I assays, found that with...
Herein, we describe a simple and promising nanoparticle oral delivery phenomenon propose pathways for absorption from the gastrointestinal tract (GIT), combining apical sodium-dependent bile acid transporter-mediated cellular uptake chylomicron transport pathways. This strategy is proven to employ bile-acid-conjugated, solid fluorescent probe nanoparticles (100 nm diameter) exclude any potential artifacts instability issues in observing measuring bioavailability. The results of vitro studies...
In early 1988, a colony of GK rats was started in Paris with progenitors issued from F35 the original reported by Goto and Kakisaki. When studied longitudinally up to 8 mo, showed as 1 mo (weaning) significantly higher basal plasma glucose (9 mM) insulin levels (doubled), altered tolerance (intravenous glucose), very poor secretory response vivo compared Wistar controls. Males females were similarly affected. Studies vitro pancreatic function carried out isolated perfused pancreas...
Abstract CPT‐11, 7‐ethyl‐10‐[4‐( 1 ‐piperidino)‐1 ‐piperidino]carbonyloxy camptothecin, is a newly developed water‐soluble camptothecin derivative now undergoing phase‐II evaluation. In an attempt to establish whether the combination of CPT‐11 with other standard anti‐cancer agents would be any benefit, we studied effects in 11 on human T‐cell leukemia cell line, MOLT‐3, culture. We used both and SN‐38 (active substance vivo ), for our study. Cells were incubated 3 days presence 2 drugs...
Transcription activator-like effector nucleases (TALENs) have recently arisen as effective tools for targeted genome engineering. Here, we report streamlined methods the construction and evaluation of TALENs based on 'Golden Gate TALEN TAL Effector Kit' (Addgene). We diminished array vector requirements increased assembly rates using six-module concatemerization. altered architecture native protein to increase nuclease activity replaced final destination with a mammalian expression/in vitro...
An epidemic of enterohemorrhagic colitis caused by Escherichia coli O157:H7 (EHEC-O157) occurred in a nursery school rural area Japan September 1996. The EHEC-O157 were isolated both from patients and houseflies collected at the school. flies suspected to be mechanical vectors pathogen. Feeding experiments showed that ingested bacteria harbored intestine continued excreted least for 3 days after feeding. Scanning electron microscopy large number adhered surface housefly mouthparts actively...
Thyroid hormone (TH) receptor (TR) expression begins early in development all vertebrates when circulating TH levels are absent or minimal, yet few developmental roles for unliganded TRs have been established. Unliganded expected to repress TH-response genes, increase tissue responsivity TH, and regulate the timing of events. Here we examined role TRα gene repression Xenopus tropicalis. We used transcription activator-like effector nuclease disruption technology generate founder animals with...
Recently, gene editing with transcription activator-like effector nucleases (TALENs) has been used in the life sciences. TALENs can be easily customized to recognize a specific DNA sequence and efficiently introduce double-strand breaks at targeted genomic locus. Subsequent non-homologous end-joining repair leads disruption by base insertion, deletion, or both. Here, readily evaluate efficacy of Xenopus laevis embryos, we performed tyrosinase (tyr) pax6 genes that are involved pigmentation...
Abstract We investigated activation of mitogen-activated protein kinase (MAPK) subtype cascades in human neutrophils stimulated by IL-1β. IL-1β induced phosphorylation and p38 MAPK kinase-3/6 (MKK3/6). Maximal was obtained stimulation cells with 300 U/ml for 10 min. Extracellular signal-regulated (ERK) faintly phosphorylated c-Jun N-terminal (JNK) not primed enhanced release superoxide (O2−) FMLP parallel increased MAPK. also O2− up-regulation CD11b CD15, both responses were inhibited...
A cellulase [endo‐β‐1,4‐ d ‐glucanase (EC 3.2.1.4)] was isolated from the hepatopancreas of abalone Haliotis discus hannai by successive chromatographies on TOYOPEARL CM‐650M, hydroxyapatite and Sephacryl S‐200 HR. The molecular mass estimated to be 66 000 Da SDS/PAGE, thus enzyme named HdEG66. hydrolytic activity HdEG66 toward carboxymethylcellulose showed optimal temperature pH at 38 °C 6.3, respectively. cDNAs encoding were amplified polymerase chain reaction an cDNA library with primers...
Massadine, a highly oxygenated alkaloid, was isolated from the marine sponge Stylissa aff. massa as an inhibitor of geranylgeranyltransferase type I (GGTase I). The structure massadine has been deduced spectral data. Massidine inhibited GGTase Candida albicans with IC(50) value 3.9 microM. [structure: see text]