A5083484332- Developmental Biology and Gene Regulation
- Telomeres, Telomerase, and Senescence
- Corneal Surgery and Treatments
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Planarian Biology and Electrostimulation
- Antimicrobial Peptides and Activities
- Ocular Surface and Contact Lens
- Silk-based biomaterials and applications
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Muscle Physiology and Disorders
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Zebrafish Biomedical Research Applications
- Nuclear Structure and Function
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Identification and Quantification in Food
- Plant-Microbe Interactions and Immunity
- Amphibian and Reptile Biology
TU Dresden
2018-2025
Max Planck Institute of Molecular Cell Biology and Genetics
2018-2025
Miami University
2023
Karolinska Institutet
2023
Canadian Society for Digital Humanities
2022
University College London
2013-2016
Institute of Structural and Molecular Biology
2013-2016
MRC Laboratory of Molecular Biology
2009
Consejo Nacional de Investigaciones Científicas y Técnicas
2006-2007
Hospital César Milstein
2007
Cellular senescence has been recently linked to the promotion of age-related pathologies, including a decline in regenerative capacity. While such capacity deteriorates with age mammals, it remains intact species as salamanders, which have an extensive repertoire regeneration and can undergo multiple episodes through their lifespan. Here we show that, surprisingly, there is significant induction cellular during salamander limb regeneration, but that rapid effective mechanisms senescent cell...
The evolution of advanced cognition in vertebrates is associated with two independent innovations the forebrain: six-layered neocortex mammals and dorsal ventricular ridge (DVR) sauropsids (reptiles birds). How these arose vertebrate ancestors remains unclear. To reconstruct forebrain tetrapods, we built a cell-type atlas telencephalon salamander Pleurodeles waltl . Our molecular, developmental, connectivity data indicate that parts sauropsid DVR trace back to tetrapod ancestors. By...
Significance Our work establishes that endogenous regulation of the activity tumor-suppressor p53 is a critical component in vertebrate limb regeneration and required for process to occur. We show down-regulation cell cycle reentry postmitotic differentiated cells, step formation progenitors regeneration. propose absence tumor-suppressors stabilize presence dominant-negative isoforms, permit by negative p53. This research sheds light onto previously unknown molecular mechanisms offers new...
Cellular senescence, a form of stable cell cycle arrest traditionally associated with tumour suppression, has been recently found to occur during mammalian development. Here, we show that senescence is an intrinsic part the developmental programme in amphibians. Programmed occurs specific structures at defined time-windows amphibian It contributes physiological degeneration pronephros and development cement gland oral cavity. In both contexts, depends on TGFβ signalling but independent...
Axolotl limb regeneration is accompanied by the transient induction of cellular senescence within blastema, structure that nucleates regeneration. The precise role this blastemal senescent cell (bSC) population, however, remains unknown. Here, through a combination gain- and loss-of-function assays, we elucidate functions molecular features in vivo. We demonstrate plays positive during axolotl creating pro-proliferative niche supports progenitor expansion blastema outgrowth. Senescent cells...
Abstract Xanthan is the major exopolysaccharide secreted by Xanthomonas spp. Despite its diverse roles in bacterial pathogenesis of plants, little known about real implication this molecule pathogenesis. In study we show that contrast to campestris pv strain 8004 (wild type), xanthan minus mutant (strain 8397) and 8396, which producing truncated xanthan, fail cause disease both Nicotiana benthamiana Arabidopsis (Arabidopsis thaliana) plants. wild type, 8397 8396 strains induce callose...
Salamanders are able to regenerate their entire limbs throughout lifespan, through a process that involves significant modulation of cellular plasticity. Limb regeneration is accompanied by the endogenous induction senescence, state irreversible cell cycle arrest associated with profound non-cell-autonomous consequences. While traditionally detrimental physiological effects, here, we show senescent cells can enhance newt limb regeneration. Through lineage tracing approach, demonstrate...
Abstract Although cyclic glucans have been shown to be important for a number of symbiotic and pathogenic bacterium–plant interactions, their precise roles are unclear. Here, we examined the role β-(1,2)-glucan in virulence black rot pathogen Xanthomonas campestris pv (Xcc). Disruption Xcc nodule development B (ndvB) gene, which encodes glycosyltransferase required glucan synthesis, generated mutant that failed synthesize extracellular was compromised model plants Arabidopsis thaliana...
In regeneration-competent vertebrates, such as salamanders, regeneration depends on the ability of various differentiated adult cell types to undergo natural reprogramming. This is rarely observed in regeneration-incompetent species mammals, providing an explanation for their poor regenerative potential. To date, little known about molecular mechanisms mediating reprogramming during regeneration. Here, we have identified extent extracellular signal-regulated kinase (ERK) activation a key...
Abstract Cardiovascular lineages develop together with kidney, smooth muscle, and limb connective tissue progenitors from the lateral plate mesoderm (LPM). How LPM initially emerges how its downstream fates are molecularly interconnected remain unknown. Here, we isolate a pan-LPM enhancer in zebrafish-specific draculin ( drl ) gene that provides specific reporter activity early gastrulation. In toto live imaging lineage tracing of -based reporters captures dynamic emergence as...
Abstract Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of amputation level. This suggests that cells in stump have awareness their spatial location, a property termed positional identity. Little known about how identity encoded, salamanders or other biological systems. Through single-cell RNAseq analysis, we identified Tig1/Rarres1 as potential determinant proximal Tig1 encodes conserved cell surface molecule,...
Abstract Background In mammals, specific brain regions such as the dentate gyrus (DG) of hippocampus and subventricular zone (SVZ) lateral ventricles harbor adult neural stem/progenitor cells (ANSPCs) that give rise to new neurons contribute structural functional plasticity. contrast, other vertebrates salamanders zebrafish exhibit a widely distributed neurogenic niches throughout brain, suggesting greater capacity in adulthood. However, mechanisms underlying this divergence potential among...
Exhibiting extreme regenerative abilities which extend to complex organs and entire limbs, salamanders have long served as research models for understanding the basis of vertebrate regeneration. Yet these organisms display additional noteworthy traits, namely extraordinary longevity, indefinite potential apparent lack traditional signs age-related decay or “negligible senescence.” Here, I examine existing studies addressing features, highlight outstanding questions, argue that constitute...
Abstract The cell type-specific expression of key transcription factors is central to development and disease. Brachyury/T/TBXT a major factor for gastrulation, tailbud patterning, notochord formation; however, how its controlled in the mammalian has remained elusive. Here, we identify complement notochord-specific enhancers gene. Using transgenic assays zebrafish, axolotl, mouse, discover three conserved Brachyury -controlling enhancers, T3 , C I human, marsupial genomes. Acting as...
Abstract Background Previous studies have suggested that macrophages are present during lens regeneration in newts, but their role the process is yet to be elucidated. Methods Here we generated a transgenic reporter line using newt, Pleurodeles waltl , traces regeneration. Furthermore, assessed early changes gene expression two newt species, Notophthalmus viridescens and . Finally, used clodronate liposomes deplete both species tested effect of subsequent secondary injury after macrophage...
Amphibians represent a diverse group of tetrapods, marked by deep divergence times between their three systematic orders and families. Studying amphibian biology through the genomics lens increases our understanding features this animal class that other terrestrial vertebrates. The need for genomic resources is more urgent than ever due to increasing threats group. are one most imperiled taxonomic groups, with approximately 41% species threatened extinction habitat loss, changes in land use...
Inheritance of a mutation in BRCA1 (breast cancer 1 early-onset) results predisposition to early-onset breast and ovarian cancer. Tumours these individuals arise after somatic or loss the wild-type allele. Loss function leads profound increase genomic instability involving accumulation mutations, DNA breaks gross chromosomal rearrangements. Accordingly, has been implicated as an important factor involved both repair lesions regulation cell-cycle checkpoints response damage. However,...
Abstract Telomere shortening places a key limitation on cell proliferation 1 . In all vertebrates explored to date, this is overcome by telomerase-dependent telomere extension. Failure maintain length results in premature ageing and functional impairments highly replicative populations as telomeres erode 2 Alternative lengthening of (ALT), telomerase-independent mechanism, compensates for loss subset human cancer lines Here, we demonstrate that the regenerative newt Pleurodeles waltl lacks...