A5044838981- Retinal Development and Disorders
- Connexins and lens biology
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Retinal and Macular Surgery
- Pluripotent Stem Cells Research
- Domain Adaptation and Few-Shot Learning
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- MicroRNA in disease regulation
- Neurogenesis and neuroplasticity mechanisms
- Intraocular Surgery and Lenses
- Epigenetics and DNA Methylation
- Retinopathy of Prematurity Studies
- interferon and immune responses
- Extracellular vesicles in disease
- Genetics and Neurodevelopmental Disorders
- Ocular Disorders and Treatments
- Congenital heart defects research
- Hedgehog Signaling Pathway Studies
- Cancer-related molecular mechanisms research
- Chromosomal and Genetic Variations
- Circular RNAs in diseases
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
Miami University
2019-2024
TU Dresden
2023
Karolinska Institutet
2023
University of Miami
2021
ABSTRACT Ocular lens development entails epithelial to fiber cell differentiation, defects in which cause congenital cataracts. We report the first single-cell multiomic atlas of development, leveraging snRNA-seq, snATAC-seq and CUT&RUN-seq discover previously unreported mechanisms fate determination cataract-linked regulatory networks. A comprehensive profile cis- trans-regulatory interactions, including for transcription factor MAF, is established across a temporal trajectory...
Abstract Background Previous studies have suggested that macrophages are present during lens regeneration in newts, but their role the process is yet to be elucidated. Methods Here we generated a transgenic reporter line using newt, Pleurodeles waltl , traces regeneration. Furthermore, assessed early changes gene expression two newt species, Notophthalmus viridescens and . Finally, used clodronate liposomes deplete both species tested effect of subsequent secondary injury after macrophage...
Lens epithelial explants are comprised of lens cells cultured in vitro on their native basement membrane, the capsule. Biologists have used to study many different cellular processes including fiber cell differentiation. In these studies, differentiation is typically measured by elongation and expression a few proteins characteristically expressed situ. Chromatin RNA was collected from either un-supplemented media or containing 50% bovine vitreous humor for one five days. ATAC-sequencing...
Cellular reprogramming resets the epigenetic landscape to drive shifts in transcriptional programmes and cell identity. The embryonic chick can regenerate a complete neural retina, after retinectomy, via retinal pigment epithelium (RPE) presence of FGF2. In this study, we systematically analysed competent RPE prior injury, during different stages reprogramming. addition changes expression genes associated with modifications reprogramming, observed dynamic histone marks bivalent chromatin...
The retinal pigment epithelium (RPE) exhibits a diverse range of plasticity across vertebrates and is potential source cells for the regeneration neurons. Embryonic amniotes possess transitory ability to regenerate neural retina through reprogramming RPE in an FGF-dependent manner. Chicken can at embryonic day 4 (E4), but competence lost by 5 (E5). To identify mechanisms that underlie loss regenerative competence, we performed RNA ATAC sequencing using E4 E5 chicken RPE, as well both stages...
Purpose: Despite strong evidence demonstrating that normal lens development requires regulation governed by microRNAs (miRNAs), the functional role of specific miRNAs in mammalian remains largely unexplored. Methods: A comprehensive analysis miRNA transcripts newborn mouse lens, exploring both differential expression between epithelial cells and fiber overall abundance, was conducted sequencing. Mouse lenses lacking each three abundantly expressed (miR-184, miR-26, miR-1) were analyzed to...
Abstract Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Here we generated transgenic newt reporter line which can be visualized vivo . Using this new tool, analyzed the location of regeneration. We uncovered early gene expression changes using bulk RNAseq two species, Notophthalmus viridescens and Pleurodeles waltl Next, used clodronate liposomes to deplete macrophages, inhibited both species....
The plasticity of human retinal pigment epithelium (RPE) has been observed during proliferative vitreoretinopathy, a defective repair process which injured RPE gives rise to fibrosis. In contrast, following injury, the embryonic chicken can be reprogrammed regenerate neural retina in fibroblast growth factor 2 (FGF2)-dependent manner. To better explore mechanisms underlying reprogramming, we used laser capture microdissection isolate RNA from (1) intact RPE, (2) transiently (t-rRPE) 6 h...
Abstract Genomic sequences residing within introns of few genes have been shown to act as enhancers affecting expression neighboring genes. We studied an autosomal recessive phenotypic continuum microphthalmia, anophthalmia and ocular coloboma, with no apparent coding-region disease-causing mutation. Homozygosity mapping several affected Jewish Iranian families, combined whole genome sequence analysis, identified a 0.5 Mb disease-associated chromosome 2q35 locus (maximal LOD score 6.8)...
Abstract The plasticity of human retinal pigment epithelium (RPE) has been observed during proliferative vitreoretinopathy, a defective repair process which injured RPE gives rise to fibrosis. In contrast, following injury, the embryonic chicken can be reprogrammed regenerate neural retina in an FGF2-dependent manner. To characterize mechanisms underlying reprogramming, we used laser capture microdissection isolate RNA from 1) intact RPE, 2) transiently (t-rRPE) 6 hours post-retinectomy, and...
Abstract Purpose Despite strong evidence demonstrating that normal lens development requires regulation governed by miRNAs, the functional role of specific miRNAs in mammalian remains largely unexplored. Methods A comprehensive analysis miRNA transcripts newborn mouse lens, exploring both differential expression between epithelial cells and fiber overall abundance was conducted miRNA-seq. Mouse lenses lacking each three abundantly expressed miRNAs: miR-184, miR-26 miR-1 were analyzed to...
ABSTRACT During tissue regeneration, proliferation, dedifferentiation and reprogramming are necessary to restore lost structures. However, it is not fully understood how metabolism intersects with these processes. Chicken embryos can regenerate their retina through retinal pigment epithelium (RPE) when treated fibroblast factor 2 (FGF2). Using transcriptome profiling, we uncovered extensive regulation of gene sets pertaining neurogenesis glycolysis throughout RPE-to-neural reprogramming. By...
Abstract Ocular lens development entails epithelial to fiber cell differentiation, defects in which cause congenital cataract. We report the first single-cell multiomic atlas of development, leveraging snRNA-seq, snATAC-seq, and CUT&RUN-seq discover novel mechanisms fate determination cataract-linked regulatory networks. A comprehensive profile cis - trans -regulatory interactions, including for transcription factor MAF, is established across a temporal trajectory differentiation....
During tissue regeneration, proliferation, dedifferentiation, and reprogramming are necessary to restore lost structures. However, it is not fully understood how metabolism intersects with these processes. Chicken embryos can regenerate their retina through retinal pigment epithelium (RPE) when treated fibroblast factor 2 (FGF2). Using transcriptome profiling, we uncovered extensive regulation of gene sets pertaining neurogenesis, glycolysis throughout RPE-to-neural reprogramming. By...
<title>Abstract</title> Background Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Methods Here we generated transgenic newt reporter line which can be visualized <italic>in vivo</italic>. Using this new tool, analyzed the location of regeneration. We uncovered early gene expression changes using bulk RNAseq two species, <italic>Notophthalmus viridescens</italic> and <italic>Pleurodeles...
ABSTRACT Background A promising avenue toward human retina regeneration lies in identifying the factors that promote cellular reprogramming to retinal neurons organisms able undergo regeneration. The embryonic chick can regenerate a complete neural retina, after retinectomy, via pigment epithelium (RPE) presence of FGF2. Cellular resets epigenetic landscape drive shifts transcriptional programs and cell identity. Here, we systematically analyzed competent RPE prior injury, during different...