Soil fumigation with chloropicrin (CP) is an effective means of overcoming continuous cropping obstacles (CCO) in Panax notoginseng and improving its yield quality. CP can change the microbial community soil. Therefore, a key step after rapid restoration soil microorganisms promotion beneficial microorganism proliferation as dominant flora. In this study, continuously cropped P. was fumigated CP, general organic fertilizer (GOF) or (MOF) used to restore fumigation. physical chemical...

Abstract Metabolic abnormality is the major feature of laryngeal squamous cell carcinoma (LSCC), however, underlying mechanism remain largely elusive. Fatty acid desaturase 1 (FADS1), as key rate-limiting enzyme polyunsaturated fatty acids (PUFAs), catalyzes dihomo-gamma-linolenic (DGLA) to arachidonic (AA). In this study, we reported that expression FADS1 was upregulated in LSCC, high closely associated with advanced clinical features and poor prognosis recurrent LSCC patients after...

The ozonolysis of aflatoxin B1 (400 μg/mL) in acetonitrile solution was conducted with an ozone concentration 6.28 mg/L at the flow rate 60 mL/min for different times. results showed that effective detoxification agent because its powerful oxidative role. Thin-layer chromatography and liquid chromatography–quadrupole time-of-flight mass spectra were applied to confirm identify products B1. A total 13 identified, 6 them main products. structural identification these provided information...

Abstract Phenyllactic acid (PLA) has gained a lot of attention due to its broad antimicrobial activity, but the mechanism antifungal action been barely reported until now. Herein, inhibitory activity PLA against Aspergillus flavus spore germination and were preliminarily investigated. Results indicated that had strong A. with minimal concentration (MIC) fungicidal (MFC) 6 12 mg/ml, respectively. As observed by scanning electron microscopy (SEM), spores displayed wrinkled shrunken appearance...

Abstract Long non-coding RNAs (lncRNAs) play an essential role in diverse biological processes and disease development. Accurate classification of lncRNAs mRNAs is important for the identification tissue- or disease-specific lncRNAs. Here, we present our tool LncDC (Long RNA detection) that able to accurately predict with XGBoost model using features extracted from sequences, secondary structures, translated proteins. Benchmarking experiments showed consistently outperformed six...

In order to elucidate the effects of different nitrogen (N), phosphorus (P), and potassium (K) fertilization timing sequences management on nutrient absorption utilization in drip irrigation cotton, field experiments were conducted from 2020 2021. There are six methods for NPK (S1-S6: 1/3Time N-1/3Time PK-1/3Time W, 1/2Time NPK-1/2Time 1/4Time W-1/4Time N-1/4Time PK-1/4Time W-1/3Time NPK-1/3Time W), among which S6 is current method sequences, S7 non N. The results showed that during main...

In a full automatic cervical cytology screening process, one of the essential steps is segmentation nuclei. Despite some progress, there need to improve sensitivity, speed, level automation, and reduce non-cellular artifacts. This paper presents practical nuclei algorithm for solving these problems. The proposed approach first preprocess V channel image from HSV color space thus allowing accentuating contrast between nuclei/leukocyte cytoplast. order overcome non-uniform illumination,...

Long non-coding RNAs (lncRNAs) play critical roles in various biological processes plants. Extensive studies utilizing high-throughput RNA sequencing have revealed that many lncRNAs are involved plant disease resistance. Oryza sativa RNase P protein 30 (OsRpp30) has been identified as a positive regulator of rice immunity against fungal and bacterial pathogens. Nevertheless, the specific functions relation to OsRpp30-mediated resistance remain elusive.We conducted comprehensive analysis...

Purpose: Despite strong evidence demonstrating that normal lens development requires regulation governed by microRNAs (miRNAs), the functional role of specific miRNAs in mammalian remains largely unexplored. Methods: A comprehensive analysis miRNA transcripts newborn mouse lens, exploring both differential expression between epithelial cells and fiber overall abundance, was conducted sequencing. Mouse lenses lacking each three abundantly expressed (miR-184, miR-26, miR-1) were analyzed to...

As one of the four most valuable animal medicines, Fel Ursi, named Xiong Dan (XD) in China, has effect clearing heat, calming liver, and brightening eyes. However, due to special source XD its high price, other animals' bile is often sold as or mixed with on market, seriously affecting clinical efficacy consumers' rights interests. In order realize identification adulteration analysis XD, UHPLC-QTOF-MS

Angelicae sinensis radix (ASR) and pubescentis (APR), as traditional herbal medicines, are often confused doped in the material market. However, identification method is to characterize whole herb with a single or few components, which do not have representation cannot realize effective utilization of unknown components. Consequently, result convincing. In addition, process time-consuming labor-intensive. To avoid confusion adulteration ASR APR well strengthen quality control improve...

Ginseng Radix et Rhizoma (GRR), Panacis Quinquefolii (PQR), Notoginseng radix rhizoma (NRR) and Platycodonis (PR) are often confused in the material market because of similar appearances characteristics. Moreover, chemical identification methods tend to characterize whole herb with regard a single or few components, which is an inaccurate representation does not demonstrate effective utilization unknown result unconvincing. In order strengthen quality control, improve efficiency, realize...

This study was targeted to investigate the effect of combination aqueous ozone (AO) and sodium citrate (SC) pretreatment on quality vacuum packaged beef stored at chilling temperature. The steers were randomly divided into five treatment groups, containing group C (steers without any treatment), DW pretreated with distilled water), AO, SC AO+SC. During processing, parameters included total Viable Count (TVC), Volatile Basic Nitrogen (TVB-N), pH, meat color, metmyoglobin (Met-Mb),...

The role of miRNAs as crucial components in carcinogenesis has been well documented. However, whether and how miR-214 influences oral cancer cells' drug resistance remains to be elucidated, its downstream targets are still under investigation. Hence, this research is aimed at determining ULK1 expression before after chemotherapy their correlations with cell growth. Human normal epithelial cells human tongue squamous carcinoma CAL-27 were cultured detect levels. It was found that...

Abstract Purpose Despite strong evidence demonstrating that normal lens development requires regulation governed by miRNAs, the functional role of specific miRNAs in mammalian remains largely unexplored. Methods A comprehensive analysis miRNA transcripts newborn mouse lens, exploring both differential expression between epithelial cells and fiber overall abundance was conducted miRNA-seq. Mouse lenses lacking each three abundantly expressed miRNAs: miR-184, miR-26 miR-1 were analyzed to...

To elucidate the variation characteristics of nitrogen (N), phosphorus (P), and potassium (K) content accumulation in cotton at different yield levels, a two-year experiment was conducted using variety Lumianyan 24 under four N treatments (506, 402.5, 299, 195.5 kg ha−1, designated as N1, N2, N3, N4, respectively). The P K fertilization ratios were (PK-M1, 25% applied squaring stage (SS) 75% bloom-bolling stages (BS)), 50%:50% (PK-M2, 50% each stage), 75%:25% (PK-M3, SS BS) 100%:0% (PK-M4,...

Significant progress has been made in research on stress tolerance trait genes of wheat crops, which greatly promoted the development crop improvement and food security. Through high-throughput sequencing technology genome-wide association analysis (GWAS), researchers have successfully identified multiple key regulatory networks related to response, especially under conditions such as drought, salinity, low temperature. The application gene editing technologies, CRISPR/Cas9, provides new...

Introduction Under the background of digitalization traditional Chinese medicine (TCM), this study aimed to realize digital identification and adulteration analysis Codonopsis Radix (CR) Stellariae (SR) based on chemical analysis. Methods This combined concepts conducted a CR SR from different batches UHPLC-QTOF-MS E . Furthermore, shared ions were extracted as their “ion characterization” after quantization. Then, data matrices unique relative screened out, top-N outputted “digital...

Occlusion is probably the most challenging part of multi-person tracking. In this paper, we exploit a new discriminative affinity model which combines deep convolution appearance features, random walk motion model, and object shape to robustly handle occlusion during long period time. Moreover, We propose hierarchical association framework based on observation that increase uncertainty when occluded over time, decompose data problem into several sub-problems. Our approach runs online from...

The goal of this study was to establish the feasibility using critical nitrogen (N) dilution curve cotton leaves for diagnosing N nutrition, so as provide a theoretical basis and guidance accurate fertilization in drip-irrigated fields. We conducted field experiments Lumianyan 24 under four different reduced treatments including (506 kg hm−2 (N1), 402.5 (N2), 299 (N3), 195.5 (N4)) between 2018 2019. subsequently measured concentration leaf dry matter (LDM). also calculated (Nc) model,...