A5049689971- Epigenetics and DNA Methylation
- Air Quality and Health Impacts
- Vehicle emissions and performance
- interferon and immune responses
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Viral Infections and Vectors
- Molecular Biology Techniques and Applications
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Air Quality Monitoring and Forecasting
- Pancreatic function and diabetes
- Cancer-related gene regulation
- Hematopoietic Stem Cell Transplantation
- Retinal Development and Disorders
- Prostate Cancer Treatment and Research
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Atmospheric chemistry and aerosols
- Pancreatic and Hepatic Oncology Research
- RNA Research and Splicing
- Zebrafish Biomedical Research Applications
TU Dresden
2016-2025
Center for Systems Biology Dresden
2015-2025
Helmholtz-Zentrum Dresden-Rossendorf
2024
OncoRay
2024
Heinrich Heine University Düsseldorf
2024
German Center for Neurodegenerative Diseases
2024
German Center for Diabetes Research
2024
Deutsches Diabetes-Zentrum e.V.
2024
Paul Langerhans Institute Dresden
2024
Oslo University Hospital
2022
Trained innate immunity fosters a sustained favorable response of myeloid cells to secondary challenge, despite their short lifespan in circulation. We thus hypothesized that trained acts via modulation hematopoietic stem and progenitor (HSPCs). Administration β-glucan (prototypical trained-immunity-inducing agonist) mice induced expansion progenitors the lineage, which was associated with elevated signaling by immune mediators, such as IL-1β granulocyte-macrophage colony-stimulating factor...
High-throughput sequencing technology enables population-level surveys of human genomic variation. Here, we examine the joint allele frequency distributions across continental populations and present an approach for combining complementary aspects whole-genome, low-coverage data targeted high-coverage data. We apply this to generated by pilot phase Thousand Genomes Project, including whole-genome 2–4× coverage 179 samples from HapMap European, Asian, African panels as well target exons 800...
Evolutionary expansion of the human neocortex reflects increased amplification basal progenitors in subventricular zone, producing more neurons during fetal corticogenesis. In this work, we analyze transcriptomes distinct progenitor subpopulations isolated by a cell polarity-based approach from developing mouse and neocortex. We identify 56 genes preferentially expressed apical radial glia that lack orthologs. Among these, ARHGAP11B has highest degree glia-specific expression. arose partial...
Abstract Salamanders serve as important tetrapod models for developmental, regeneration and evolutionary studies. An extensive molecular toolkit makes the Mexican axolotl ( Ambystoma mexicanum ) a key representative salamander investigations. Here we report sequencing assembly of 32-gigabase-pair genome using an approach that combined long-read sequencing, optical mapping development new assembler (MARVEL). We observed size expansion introns intergenic regions, largely attributable to...
Trained innate immunity, induced via modulation of mature myeloid cells or their bone marrow progenitors, mediates sustained increased responsiveness to secondary challenges. Here, we investigated whether anti-tumor immunity can be enhanced through induction trained immunity. Pre-treatment mice with β-glucan, a fungal-derived prototypical agonist resulted in diminished tumor growth. The effect β-glucan-induced was associated transcriptomic and epigenetic rewiring granulopoiesis neutrophil...
The oncofetal mRNA-binding protein IGF2BP1 and the transcriptional regulator SRF modulate gene expression in cancer. In cancer cells, we demonstrate that promotes of a conserved N6-methyladenosine (m6A)-dependent manner by impairing miRNA-directed decay mRNA. This results enhanced SRF-dependent activity tumor cell growth invasion. At post-transcriptional level, sustains various SRF-target genes. majority these SRF/IGF2BP1-enhanced genes, including PDLIM7 FOXK1, show upregulation with...
The transition from maternal to zygotic control is fundamental the life cycle of all multicellular organisms. It widely believed that genomes are transcriptionally inactive fertilization until genome activation (ZGA). Thus, earliest genes expressed probably support rapid cell divisions precede morphogenesis and, if so, might be evolutionarily conserved. Here, we identify transcripts in zebrafish, Danio rerio, through metabolic labeling and purification RNA staged embryos. Surprisingly,...
Abstract The planarian Schmidtea mediterranea is an important model for stem cell research and regeneration, but adequate genome resources this species have been lacking. Here we report a highly contiguous assembly of S. , using long-read sequencing de novo assembler (MARVEL) enhanced low-complexity reads. polymorphic repetitive, harbours novel class giant retroelements. Furthermore, the lacks number conserved genes, including critical components mitotic spindle checkpoint, planarians...
Genome integrity is continuously challenged by the DNA damage that arises during normal cell metabolism. Biallelic mutations in genes encoding genome surveillance enzyme ribonuclease H2 (RNase H2) cause Aicardi-Goutières syndrome (AGS), a pediatric disorder shares features with autoimmune disease systemic lupus erythematosus (SLE). Here we determined heterozygous parents of AGS patients exhibit an intermediate phenotype and demonstrated genetic association between rare RNASEH2 sequence...
Macrophages populate every organ during homeostasis and disease, displaying features of tissue imprinting heterogeneous activation. The disconnected picture macrophage biology that has emerged from these observations is a barrier for integration across models or with in vitro activation paradigms. We set out to contextualize heterogeneity mouse tissues inflammatory conditions, specifically aiming define common framework built predictive model which we mapped the macrophages 12 25 biological...
Pancreatic islet beta cell failure causes type 2 diabetes in humans. To identify transcriptomic changes diabetic islets, the Innovative Medicines Initiative for Diabetes: Improving beta-cell function and identification of diagnostic biomarkers treatment monitoring Diabetes (IMIDIA) consortium ( www.imidia.org ) established a comprehensive, unique multicentre biobank human islets pancreas tissues from organ donors metabolically phenotyped pancreatectomised patients (PPP).Affymetrix...
The HIV restriction factor, SAMHD1 (SAM domain and HD domain-containing protein 1), is a triphosphohydrolase that degrades deoxyribonucleoside triphosphates (dNTPs). Mutations in cause Aicardi-Goutières syndrome (AGS), an inflammatory disorder shares phenotypic similarity with systemic lupus erythematosus, including activation of antiviral type 1 interferon (IFN). To further define the pathomechanisms underlying autoimmunity AGS due to mutations, we investigated physiological properties SAMHD1.
Aicardi-Goutie `res syndrome (AGS), a hereditary autoimmune disease, clinically and biochemically overlaps with systemic lupus erythematosus (SLE) and, like SLE, is characterized by spontaneous type I interferon (IFN) production.The finding that defects of intracellular nucleases cause AGS led to the concept accumulation nucleic acids triggers inappropriate production IFN autoimmunity.AGS can also be caused SAMHD1, 3 0 exonuclease deoxynucleotide (dNTP) triphosphohydrolase.Human SAMHD1 an...
Human brains are prone to neurodegeneration, given that endogenous neural stem/progenitor cells (NSPCs) fail support neurogenesis. To investigate the molecular programs potentially mediating neurodegeneration-induced NSPC plasticity in regenerating organisms, we generated an Amyloid-β42 (Aβ42)-dependent neurotoxic model adult zebrafish brain through cerebroventricular microinjection of cell-penetrating Aβ42 derivatives. deposits neurons and causes phenotypes reminiscent amyloid...
Prostate cancer is the second most common among men worldwide. Alterations in DNA methylation pattern can be one of leading causes for prostate formation. This study first high-throughput sequencing investigating genome-wide patterns a large cohort 51 tumor and 53 benign samples using methylated immunoprecipitation sequencing. Comparative analyses identified more than 147,000 cancer-associated epigenetic alterations. In addition, global show significant differences based on TMPRSS2-ERG...