A5068769876- Cancer, Hypoxia, and Metabolism
- Radiopharmaceutical Chemistry and Applications
- RNA modifications and cancer
- Crystallization and Solubility Studies
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Medical Imaging Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- X-ray Diffraction in Crystallography
- Cardiovascular Function and Risk Factors
- Cardiac Imaging and Diagnostics
- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Metabolism, Diabetes, and Cancer
- Peroxisome Proliferator-Activated Receptors
- Eating Disorders and Behaviors
- Sepsis Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Medical Imaging and Pathology Studies
University Hospital Carl Gustav Carus
2006-2025
TU Dresden
2006-2025
National Center for Tumor Diseases
2023-2025
German Cancer Research Center
2023
Heidelberg University
2023
ETH Zurich
2012-2022
Carl Gustav Carus-Institut
2022
Institute of Cell Biology
2012
University of Ferrara
2008
Czech Academy of Sciences, Institute of Physiology
2007
Trained innate immunity fosters a sustained favorable response of myeloid cells to secondary challenge, despite their short lifespan in circulation. We thus hypothesized that trained acts via modulation hematopoietic stem and progenitor (HSPCs). Administration β-glucan (prototypical trained-immunity-inducing agonist) mice induced expansion progenitors the lineage, which was associated with elevated signaling by immune mediators, such as IL-1β granulocyte-macrophage colony-stimulating factor...
Trained innate immunity, induced via modulation of mature myeloid cells or their bone marrow progenitors, mediates sustained increased responsiveness to secondary challenges. Here, we investigated whether anti-tumor immunity can be enhanced through induction trained immunity. Pre-treatment mice with β-glucan, a fungal-derived prototypical agonist resulted in diminished tumor growth. The effect β-glucan-induced was associated transcriptomic and epigenetic rewiring granulopoiesis neutrophil...
Dietary obesity is a major factor in the development of type 2 diabetes and associated with intra-adipose tissue hypoxia activation hypoxia-inducible 1α (HIF1α). Here we report that, mice, Hif1α visceral white adipocytes critical to maintain dietary pathologies, including glucose intolerance, insulin resistance, cardiomyopathy. This function linked its capacity suppress β-oxidation, part, through transcriptional repression sirtuin ( Sirt2 ) NAD + -dependent deacetylase. Reduced directly...
Radiotherapy is one of the curative treatment options for localized prostate cancer (PCa).The potential radiotherapy mediated by irradiation-induced oxidative stress and DNA damage in tumor cells.However, PCa radiocurability can be impeded resistance mechanisms normal tissue toxicity.Metabolic reprogramming major hallmarks progression therapy resistance.Specific metabolic features might serve as therapeutic targets radiosensitization biomarkers Ivyspring International Publisheridentifying...
Microglia, the parenchymal immune cells of central nervous system, orchestrate neuroinflammation in response to infection or damage, and promote tissue repair. However, aberrant microglial responses are integral neurodegenerative diseases critically contribute disease progression. Thus, it is important elucidate how microglia - mediated regulated by endogenous factors. Here, we explored effect Nerve Growth Factor (NGF), an abundant neurotrophin, on inflammatory responses. NGF, via its high...
The causative role of inflammation in hypertension-related cardiovascular diseases is evident and calls for development specific immunomodulatory therapies. We tested the therapeutic efficacy mechanisms action developmental endothelial locus-1 (DEL-1), an endogenous antiinflammatory factor, angiotensin II- (ANGII-) deoxycorticosterone acetate-salt-induced (DOCA-salt-induced) organ damage hypertension. By using mice with overexpression DEL-1 (EC-Del1 mice) performing preventive interventional...
Abstract Background Chemotherapy (CT) is central to the treatment of triple negative breast cancer (TNBC), but drug toxicity and resistance place strong restrictions on regimes. Fasting sensitizes cells a range chemotherapeutic agents also ameliorates CT-associated adverse effects. However, molecular mechanism(s) by which fasting, or short-term starvation (STS), improves efficacy CT poorly characterized. Methods The differential responses near normal cell lines combined STS were assessed...
<p dir="ltr">Objective: Progression of prediabetes to type 2 diabetes has been associated with beta cell dysfunction, whereas its remission normoglycemia related improvement insulin sensitivity. To understand the mechanisms and identify potential biomarkers trajectories, we compared proteomics metabolomics profile people progressing or reversing within a year. </p><p dir="ltr">Research Design Methods: The fasting plasma concentrations 1389 proteins fasting, 30 min 120...
OBJECTIVE Progression of prediabetes to type 2 diabetes has been associated with β-cell dysfunction, whereas its remission normoglycemia related improvement insulin sensitivity. To understand the mechanisms and identify potential biomarkers trajectories, we compared proteomics metabolomics profile people progressing or reversing within 1 year. RESEARCH DESIGN AND METHODS The fasting plasma concentrations 1,389 proteins fasting, 30-min, 120-min post–oral glucose tolerance test (OGTT) 152...
<p dir="ltr">Objective: Progression of prediabetes to type 2 diabetes has been associated with beta cell dysfunction, whereas its remission normoglycemia related improvement insulin sensitivity. To understand the mechanisms and identify potential biomarkers trajectories, we compared proteomics metabolomics profile people progressing or reversing within a year. </p><p dir="ltr">Research Design Methods: The fasting plasma concentrations 1389 proteins fasting, 30 min 120...
Abstract Background Hypoxia is a critical physiological and pathological condition known to influence various cellular processes, including steroidogenesis. While previous studies, our own, have highlighted the regulatory effects of Hypoxia-Inducible Factor 1α (HIF1α) on steroid production, specific molecular mechanisms remain poorly understood. This study investigates role hypoxia HIF1α in biosynthesis across multiple experimental models during acute exposure low oxygen levels. Methods To...
Abstract The immaturity of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is a major limitation for their use in drug screening to identify pro-arrhythmogenic or cardiotoxic molecules. Here, we demonstrate an approach that combines lipid-enriched maturation medium with high concentration calcium, nanopatterning culture surfaces and electrostimulation generate iPSC-CMs advanced electrophysiological, structural metabolic phenotypes. Systematic testing reveals the key...
Background: Ventricular arrhythmia and sudden cardiac death are the most common lethal complications after myocardial infarction. Antiarrhythmic pharmacotherapy remains a clinical challenge novel concepts highly desired. Here, we focus on cardioprotective CNP (C-type natriuretic peptide) as antiarrhythmic principle. We hypothesize that effects of mediated by PDE2 (phosphodiesterase 2), which has unique property to be stimulated cGMP primarily hydrolyze cAMP. Thus, might promote beneficial...
Rheumatoid arthritis is an inflammatory joint disease in which synovial iron deposition has been described. Transferrin receptor 2 (Tfr2) represents a critical regulator of systemic levels. Loss Tfr2 function humans and mice results overload. As contributes to processes, we investigated whether Tfr2-deletion affects the pathogenesis iron-dependent manner.Using global conditional genetic disruption Tfr2, assessed relevance K/BxN serum-transfer (STA) macrophage polarization.Male Tfr2-/-...
Abstract Myotonic dystrophy type 2 (DM2) is a tetranucleotide CCTG repeat expansion disease associated with an increased prevalence of autoimmunity. Here, we identified elevated I interferon (IFN) signature in peripheral blood mononuclear cells and primary fibroblasts DM2 patients as trigger chronic immune stimulation. Although RNA-repeat accumulation was prevalent the cytosol DM2-patient fibroblasts, type-I IFN release did not depend on innate RNA sensors but rather DNA sensor cGAS...
The quality of biological samples used in metabolomics research is significantly influenced by preanalytical factors, such as the timing centrifugation and freezing. This study aimed to evaluate how like delays freezing, affect research. Blood samples, collected various tube types, were subjected controlled pre- postcentrifugation delays. Metabolite levels quantified using NMR spectroscopy fitted linear mixed models predict changes metabolite concentrations over time. results showed that...
Glucocorticoids regulate hematopoiesis, but how chronic elevation of endogenous glucocorticoid production affects hematopoietic stem cell (HSC) function and immune development remains incompletely understood. Using an adrenocortical cell-specific HIF1α (Hypoxia inducible Factor-1α)-deficient mouse model (P2H1Ad.Cortex) resulting in elevated (GC) levels, we here demonstrate that sustained GC exposure promotes progenitor (HSPC) expansion while shifting HSCs toward a more quiescent...
Enhancers are genomic regulatory elements conferring spatiotemporal and signal-dependent control of gene expression. Recent evidence suggests that enhancers can generate noncoding enhancer RNAs, but their (patho)biological functions remain largely elusive.We performed chromatin immunoprecipitation-coupled sequencing histone marks combined with RNA left ventricular biopsies from experimental genetic mouse models human cardiac hypertrophy to identify transcripts revealing localization,...
Corticosteroids regulate vital processes, including stress responses, systemic metabolism, and blood pressure. Here, we show that corticosteroid synthesis is related to the polyunsaturated fatty acid (PUFA) content of mitochondrial phospholipids in adrenocortical cells. Inhibition rate-limiting enzyme PUFA synthesis, desaturase 2 (FADS2), leads perturbations lipidome diminishes steroidogenesis. Consistently, mitochondria Fads2 −/− mice fed a diet with low concentration are structurally...