A5001177197- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Diabetes and associated disorders
- Metabolism, Diabetes, and Cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Diet, Metabolism, and Disease
- Hearing, Cochlea, Tinnitus, Genetics
- Growth Hormone and Insulin-like Growth Factors
- RNA modifications and cancer
- Regulation of Appetite and Obesity
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Diabetes Management and Research
- Diabetes Treatment and Management
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and disorders
- Toxic Organic Pollutants Impact
- Effects and risks of endocrine disrupting chemicals
- Genetics and Neurodevelopmental Disorders
- Metabolism and Genetic Disorders
- Connexins and lens biology
- Lipoproteins and Cardiovascular Health
- Liver Disease Diagnosis and Treatment
- Cellular transport and secretion
- Diet and metabolism studies
Slovak Academy of Sciences
2014-2023
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2014-2023
Comenius University Bratislava
2021-2023
Centre for Advanced Material Application of the Slovak Academy of Sciences
2023
Biomedical Research Center of the Slovak Academy of Sciences
2019-2022
Key points Considerable controversy exists regarding the role of irisin, a putative exercise‐induced myokine, in human metabolism. We therefore studied irisin and its precursor Fndc5 obesity, type 2 diabetes exercise. Complex clinical studies combined with cell culture work revealed that /irisin was decreased vivo , but not muscle cells vitro indicating diabetes‐related factor(s) regulate . Several attributes diabetes, such as hyperglycaemia, triglyceridaemia, visceral adiposity...
Dietary obesity is a major factor in the development of type 2 diabetes and associated with intra-adipose tissue hypoxia activation hypoxia-inducible 1α (HIF1α). Here we report that, mice, Hif1α visceral white adipocytes critical to maintain dietary pathologies, including glucose intolerance, insulin resistance, cardiomyopathy. This function linked its capacity suppress β-oxidation, part, through transcriptional repression sirtuin ( Sirt2 ) NAD + -dependent deacetylase. Reduced directly...
KCNJ11 mutations cause permanent neonatal diabetes through pancreatic ATP-sensitive potassium channel activation. 90% of patients successfully transfer from insulin to oral sulfonylureas with excellent initial glycaemic control; however, whether this control is maintained in the long term unclear. Sulfonylurea failure seen about 44% people type 2 after 5 years treatment. Therefore, we did a 10-year multicentre follow-up study large international cohort address key questions relating...
The development of obesity and associated insulin resistance involves a multitude gene products, including proteins involved in lipid synthesis oxidation, thermogenesis, cell differentiation. genes encoding these are essence the blueprints that we have inherited from our parents. However, what determines way which interpreted is largely dictated by collection environmental factors. nutrients consume among most influential During early stages evolutionary development, functioned as primitive...
Abstract To study the mechanisms responsible for hypotriglyceridemic effect of marine oils, we monitored effects high dietary intake n−3 PUFA on hepatic and muscular β‐oxidation, plasma leptin concentration, receptor gene expression, in vivo insulin action. Two groups male Wistar rats were fed either a high‐fat diet [28% (w/w) saturated fat] or containing 10% 18% fat 3 wk. The was accompanied by increased oxidation palmitoyl‐CoA (125%, P <0.005) palmitoyl‐ l ‐carnitine (480%, <0.005)....
Mutations in the KCNJ11 and ABCC8 genes encoding pancreatic beta-cell K(ATP) channel have recently been shown to be most common cause of permanent neonatal diabetes mellitus (PNDM). Information regarding frequency PNDM has based mainly on nonpopulation or short-term collections only. Thus, aim this study was identify incidence Slovakia switch patients sulfonylurea (SU) where applicable.We searched for Slovak Children Diabetes Registry. In insulin-treated who matched clinical criteria PNDM,...
A recent genome-wide association study identified hepatocyte nuclear factor 1-α (HNF1A) as a key regulator of fucosylation. We hypothesized that loss-of-function HNF1A mutations causal for maturity-onset diabetes the young (MODY) would display altered fucosylation N-linked glycans on plasma proteins and glycan biomarkers could improve efficiency diagnosis HNF1A-MODY. In pilot comparison 33 subjects with HNF1A-MODY 41 type 2 diabetes, 15 29 measurements differed between two groups. The...
Obesity is a well-known risk factor for the development of secondary complications such as type 2 diabetes. However, only part obese population develops metabolic disorders. Here, we identify transcription retinoid-related orphan receptor gamma (RORγ) negative regulator adipocyte differentiation through expression its newly identified target gene matrix metalloproteinase 3. In vivo progenitor cells from Rorγ-deficient mice enhanced and Rorγ(-/-) show decreased sizes. These small adipocytes...
Impairment of translation initiation and its regulation within the integrated stress response (ISR) related unfolded-protein has been identified as a cause several multisystemic syndromes. Here, we link MEHMO syndrome, whose genetic etiology was unknown, to this group disorders. is rare X-linked syndrome characterized by profound intellectual disability, epilepsy, hypogonadism hypogenitalism, microcephaly, obesity. We have C-terminal frameshift mutation (Ile465Serfs) in EIF2S3 gene three...
High dietary intake of saturated fat impairs insulin sensitivity and lipid metabolism. The influence fatty acid chain length, however, is not yet fully understood, but evidence exists for different effects long-chain (LC) versus medium-chain (MC) acids (FA).To investigate the FA male Wistar rats were fed high-fat diets containing triacylglycerols composed either MC- or LCFA 4 weeks; maintenance diet served as a control. animals underwent euglycemic hyperinsulinemic clamping oral metabolic...
To examine the regulatory aspects of zinc-α2-glycoprotein (ZAG) association with obesity-related insulin resistance. ZAG mRNA and protein were analyzed in subcutaneous adipose tissue (AT) circulation lean, obese, prediabetic, type 2 diabetic men; both visceral AT explored lean extremely obese. Clinical ex vivo findings corroborated by results vitro silencing experiment. Subcutaneous was reduced obesity, a trend to further decrease prediabetes diabetes. 3.3-fold higher than individuals. All...
OBJECTIVE ABCC8 mutations cause neonatal diabetes mellitus that can be transient (TNDM) or, less commonly, permanent (PNDM); ∼90% of individuals treated with oral sulfonylureas instead insulin. Previous studies suggested people ABCC8-PNDM require lower sulfonylurea doses and have milder neurological features than those KCNJ11-PNDM. However, these were short-term included combinations ABCC8-TNDM. We aimed to assess the long-term glycemic outcomes in sulfonylurea-treated ABCC8-PNDM. RESEARCH...
The aim of this study was to determine the effectiveness Type 1 Diabetes Genetic Risk Score (T1D GRS) for prioritisation children with newly diagnosed hyperglycaemia genetic testing monogenic diabetes. Methods: A cohort 808 and adolescents were collected. All underwent standard clinical follow-up based on knowledge means accessible at time. In in 189 control subjects (165 diabetes patients 24 healthy individuals), we assessed T1D GRS2 10 SNP GRS scores. We cut-off our investigated its...
Abstract Objectives Mitochondrial DNA (mtDNA) mutations account for up to 5% of hereditary hearing loss cases. Most commonly, the m.3243A>G mtDNA variant contributes rare monogenic MIDD (Maternally Inherited Diabetes and Deafness) or MELAS (Mitochondrial Encephalopathy, Lactic Acidosis, Stroke-like episodes) syndromes. Different proportions mutated (heteroplasmy) among affected tissues result in variability clinical manifestation severity phenotype. The aim presented study was establish...
This study was aimed to evaluate possible obesogenic and diabetogenic impact of highly increased serum level persistent organochlorinated pollutants POPs, such as polychlorinated biphenyls (PCBs), dichlorodiethyl-dichloroethylene (p,p'-DDE), hexachlorobenzene (HCB), on the obesity markers (cholesterol triglyceride in serum, body mass index [BMI]) diabetes (fasting glucose fasting insulin serum) inhabitants Eastern Slovakia.In young (21-40 years) males (n=248) females (n=330) well old (41-75...
Carnosine is a naturally present dipeptide abundant in skeletal muscle and an over-the counter food additive. Animal data suggest role of carnosine supplementation the prevention treatment obesity, insulin resistance, type 2 diabetes cardiovascular disease but only limited human exists.Samples vastus lateralis were obtained by needle biopsy. We measured levels (high-performance liquid chromatography), % body fat (bioimpedance), abdominal subcutaneous visceral adiposity (magnetic resonance...
Recently, the genetic cause of several syndromic forms glycemia dysregulation has been described. One them, MEHMO syndrome, is a rare X-linked syndrome recently linked to EIF2S3 gene mutations. characterized by Mental retardation, Epilepsy, Hypogonadism/hypogenitalism, Microcephaly, and Obesity. Moreover, patients with had also diabetes endocrine phenotype, but detailed information missing. We aimed provide more details on phenotype in two previously reported male probands carrying...
Abstract Objective: Many adipokines, inflammatory cytokines, and other proteins produced by adipose tissue have been shown to be involved in the development of obesity‐related insulin resistance. Nevertheless, new factors that play an important role these processes are still emerging. Therefore, we screened level 120 different biopsies subcutaneous (ScAT) lean obese subjects. Research Methods Procedures: All studied volunteers (12 with BMI >30 6 <25 kg/m 2 ) were young, clinically...