A5013048035- Advanced MRI Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Advanced NMR Techniques and Applications
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Cardiovascular Function and Risk Factors
- Metabolomics and Mass Spectrometry Studies
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Diabetes Management and Research
- Metabolism and Genetic Disorders
- Medical Imaging Techniques and Applications
- Growth Hormone and Insulin-like Growth Factors
- Biochemical effects in animals
- Lanthanide and Transition Metal Complexes
- Advanced Neuroimaging Techniques and Applications
- Atomic and Subatomic Physics Research
- Diet, Metabolism, and Disease
- Cardiovascular and exercise physiology
- Cardiovascular Disease and Adiposity
- Liver Disease and Transplantation
- MRI in cancer diagnosis
- Glycogen Storage Diseases and Myoclonus
- Mitochondrial Function and Pathology
Medical University of Vienna
2016-2025
Vienna General Hospital
2003-2025
Christian Doppler Laboratory for Thermoelectricity
2015-2023
Karl Landsteiner Society
2021
University of Vienna
1995-2020
Wienerberger (Czechia)
2020
Hanusch Hospital
2016
Collaborative Research Group
2016
Yamagata University
2013
VPDiagnostics (United States)
2013
To examine the mechanism by which metformin lowers endogenous glucose production in type 2 diabetic patients, we studied seven subjects, with fasting hyperglycemia (15.5 +/- 1.3 mmol/l), before and after 3 months of treatment. Seven healthy matched for sex, age, BMI, served as control subjects. Rates net hepatic glycogenolysis, estimated 13C nuclear magnetic resonance spectroscopy, were combined estimates contributions to gluconeogenesis measured labeling blood 2H from ingested 2H2O. Glucose...
Inhibition of tumor angiogenesis through blockade the vascular endothelial growth factor (VEGF) signaling pathway is a novel treatment modality in oncology. Preclinical findings suggest that long-term clinical outcomes may improve with additional proangiogenic receptor tyrosine kinases: platelet-derived receptors (PDGFR) and fibroblast (FGFR). BIBF 1120 an indolinone derivative potently blocking VEGF (VEGFR), PDGFR FGFR kinase activity enzymatic assays (IC(50), 20-100 nmol/L). inhibits...
Insulin resistance, a major factor in the pathogenesis of type 2 diabetes mellitus, is due mostly to decreased stimulation glycogen synthesis muscle by insulin. The primary rate-controlling step responsible for decrease not known, although hexokinase activity and glucose transport have been implicated.
Plasma concentrations of amino acids are frequently elevated in insulin-resistant states, and a protein-enriched diet can impair glucose metabolism. This study examined effects short-term plasma acid (AA) elevation on whole-body disposal cellular insulin action skeletal muscle. Seven healthy men were studied for 5.5 h during euglycemic (5.5 mmol/l), hyperinsulinemic (430 pmol/l), fasting glucagon (65 ng/l), growth hormone (0.4 μg/l) somatostatin clamp tests the presence low (∼1.6 mmol/l)...
Key points Considerable controversy exists regarding the role of irisin, a putative exercise‐induced myokine, in human metabolism. We therefore studied irisin and its precursor Fndc5 obesity, type 2 diabetes exercise. Complex clinical studies combined with cell culture work revealed that /irisin was decreased vivo , but not muscle cells vitro indicating diabetes‐related factor(s) regulate . Several attributes diabetes, such as hyperglycaemia, triglyceridaemia, visceral adiposity...
The translation of MRS to clinical practice has been impeded by the lack technical standardization. There are multiple methods acquisition, post‐processing, and analysis whose details greatly impact interpretation results. These often not fully reported, making it difficult assess studies on a standardized basis. This hampers reviewing manuscripts, limits reproducibility study results, complicates meta‐analysis literature. In this paper consensus group experts provides minimum guidelines for...
Decreased skeletal muscle glucose disposal and increased endogenous production (EGP) contribute to postprandial hyperglycemia in type 2 diabetes, but the contribution of hepatic glycogen metabolism remains uncertain. Hepatic EGP were monitored diabetic patients nondiabetic volunteer control subjects (CON) after mixed meal ingestion during hyperglycemic-hyperinsulinemic-somatostatin clamps applying 13C nuclear magnetic resonance spectroscopy (NMRS) variable infusion dual-tracer technique....
Muscular insulin resistance is frequently characterized by blunted increases in glucose-6-phosphate (G-6-P) reflecting impaired glucose transport/phosphorylation. These abnormalities likely relate to excessive intramyocellular lipids and mitochondrial dysfunction. We hypothesized that alterations action function should be present even nonobese patients with well-controlled type 2 diabetes mellitus (T2DM).We measured G-6-P, ATP synthetic flux (i.e., synthesis) lipid contents of skeletal...
Insulin resistance correlates with intramyocellular lipid content (IMCL) and plasma free fatty acids (FFAs) was recently linked to mitochondrial dysfunction. We examined the underlying relationships by measuring skeletal muscle ATP synthase flux, glucose transport/phosphorylation, IMCL in response different insulin FFA concentrations. Healthy men were studied twice during hyperinsulinemic-euglycemic clamps (LIP) or without (CON) infusion (plasma FFA: CON ∼36 vs. LIP ∼1,034 μmol/l, P <...
OBJECTIVE Steatosis associates with insulin resistance and may even predict type 2 diabetes cardiovascular complications. Because muscular relates to myocellular fat deposition disturbed energy metabolism, we hypothesized that reduced hepatic ATP turnover (fATP) underlies elevated hepatocellular lipid (HCL) contents. RESEARCH DESIGN AND METHODS We measured fATP using 31P magnetic resonance spectroscopy in patients age- body mass–matched controls. Peripheral (M M/I) (suppression of endogenous...
Abstract BACKGROUND: Because of intratumoral heterogeneity, diffusely infiltrating gliomas that lack significant contrast enhancement on magnetic resonance imaging are prone to tissue sampling error. Subsequent histologic undergrading may delay adjuvant treatments. 5‐Aminolevulinic acid (5‐ALA) leads accumulation fluorescent porphyrins in malignant glioma tissue, and is currently used for resection gliomas. The aim this study was clarify whether 5‐ALA might serve as marker visualization...
The initial effects of free fatty acids (FFAs) on glucose transport/phosphorylation were studied in seven healthy men the presence elevated (1.44 +/- 0.16 mmol/l), basal (0.35 0.06 and low (<0.01 mmol/l; control) plasma FFA concentrations (P < 0.05 between all groups) during euglycemic-hyperinsulinemic clamps. Concentrations glucose-6-phosphate (G-6-P), inorganic phosphate (Pi), phosphocreatine, ADP, pH calf muscle measured every 3.2 min for 180 by using 31P nuclear magnetic...
Increased hepatocellular lipids relate to insulin resistance and are typical for individuals with type 2 diabetes mellitus (T2DM). Steatosis T2DM have been further associated impaired muscular adenosine triphosphate (ATP) turnover indicating reduced mitochondrial fitness. Thus, we tested the hypothesis that hepatic energy metabolism could be even in metabolically well-controlled T2DM. We measured lipid volume fraction (HLVF) absolute concentrations of gammaATP, inorganic phosphate (Pi),...
Objective Carnosine is a naturally present dipeptide in humans and an over‐the counter food additive. Evidence from animal studies supports the role for carnosine prevention treatment of diabetes cardiovascular disease, yet there limited human data. This study investigated whether supplementation individuals with overweight or obesity improves risk factors. Methods In double‐blind randomized pilot trial nondiabetic (age 43 ± 8 years; body mass index 31 4 kg/m 2 ), 15 were randomly assigned...
With a 40‐year history of use for in vivo studies, the terminology used to describe methodology and results magnetic resonance spectroscopy (MRS) has grown substantially is not consistent many aspects. Given platform offered by this special issue on advanced MRS methodology, authors decided implicated terms, pinpoint differences their meanings suggest specific uses or definitions. This work covers terms all aspects MRS, starting from description MR signal its theoretical basis acquisition...
Abstract Hepatic steatosis develops when lipid influx and production exceed the liver’s ability to utilize/export triglycerides. Obesity promotes is characterized by leptin resistance. A role of in hepatic handling highlighted observation that recombinant reverses hypoleptinemic patients with lipodystrophy an unknown mechanism. Since mainly functions via CNS signaling, we here examine rats whether regulates flux brain a series stereotaxic infusion experiments. We demonstrate protects from...
Depletion of muscle glycogen is considered a limiting performance factor during prolonged exercise, whereas the role intramyocellular lipid (IMCL) pool not yet fully understood. We examined 1) and utilization 2) resynthesis lipids recovery, 3) changes in content between nonexercising exercising muscles recovery. Subjects ran on treadmill at submaximal intensity until exhaustion. Glycogen concentrations were assessed thigh, calf, forearm muscle, IMCL was measured soleus using magnetic...
Insulin resistance is frequently associated with increased lipid content in muscle and liver. excess stimulates tissue accumulation. To examine the effects of insulin improved glycemia on sensitivity intracellular lipids, we performed stepped (1, 2, 4 mU · min−1 kg−1) hyperinsulinemic-euglycemic clamps eight type 2 diabetic six nondiabetic control subjects at baseline after 12 67 h insulin-mediated near-normoglycemia (118 ± 7 mg/dl). Intrahepatocellular lipids (IHCLs) intramyocellular...
Women with previous gestational diabetes (pGDM) are frequently insulin-resistant, which could relate to intramyocellular lipid content (IMCL). IMCL were measured 1H nuclear magnetic resonance spectroscopy in soleus (IMCL-S) and tibialis-anterior muscles (IMCL-T) of 39 pGDM (32 ± 2 years, waist-to-hip ratio 0.81 0.01) 22 women normal glucose tolerance (NGT; 31 1 0.76 0.02) at 4–6 months after delivery. Body fat mass (BFM) was assessed from bioimpedance analysis, insulin sensitivity index...