A5033836686- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Helicobacter pylori-related gastroenterology studies
- Nutrition and Health in Aging
- Clinical Nutrition and Gastroenterology
- Gastric Cancer Management and Outcomes
- Multiple and Secondary Primary Cancers
- Diabetes and associated disorders
- Diet and metabolism studies
- Pancreatitis Pathology and Treatment
- Lung Cancer Treatments and Mutations
- Metabolism and Genetic Disorders
- Pediatric health and respiratory diseases
- Advanced Proteomics Techniques and Applications
- Bone health and treatments
- Digestive system and related health
- Pancreatic function and diabetes
- Muscle metabolism and nutrition
- Stoma care and complications
- Bacteriophages and microbial interactions
- Proteoglycans and glycosaminoglycans research
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Surgical Treatments
National Institute for Bioprocessing Research and Training
2011-2024
University of Gothenburg
2014-2019
AstraZeneca (Sweden)
2019
Polskie Towarzystwo Gastroenterologii
2015
Jesuit University Ignatianum
2015
University College Dublin
2011-2013
St. Vincent's University Hospital
2013
BlackRock (United States)
2011-2012
All immunoglobulin G molecules carry N-glycans, which modulate their biological activity. Changes in N-glycosylation of IgG associate with various diseases and affect the activity therapeutic antibodies intravenous immunoglobulins. We have developed a novel 96-well protein monolithic plate used it to rapidly isolate from plasma 2298 individuals three isolated human populations. N-glycans were released by PNGase F, labeled 2-aminobenzamide analyzed hydrophilic interaction chromatography...
The mucin O-glycosylation of 10 individuals with and without gastric disease was examined in depth order to generate a structural map human glycosylation. In the stomach, these mucins their protect epithelial surface from acidic juice provide first point interaction for pathogens such as Helicobacter pylori, reported cause gastritis, duodenal ulcers cancer. rational present study that pathogen may come contact with. An enormous diversity glycosylation found, which varied both between within...
One of today's key challenges is the ability to decode functions complex carbohydrates in various biological contexts. To generate high-quality glycomics data a high-throughput fashion, we developed robotized and low-cost N-glycan analysis platform for glycoprofiling immunoglobulin G antibodies (IgG), which are central players immune system vital importance biopharmaceutical industry. The features include (a) rapid IgG affinity purification sample concentration, (b) protein denaturation...
A recent genome-wide association study identified hepatocyte nuclear factor 1-α (HNF1A) as a key regulator of fucosylation. We hypothesized that loss-of-function HNF1A mutations causal for maturity-onset diabetes the young (MODY) would display altered fucosylation N-linked glycans on plasma proteins and glycan biomarkers could improve efficiency diagnosis HNF1A-MODY. In pilot comparison 33 subjects with HNF1A-MODY 41 type 2 diabetes, 15 29 measurements differed between two groups. The...
The majority of human proteins are post-translationally modified by covalent addition one or more complex oligosaccharides (glycans). Alterations in glycosylation processing associated with numerous diseases and glycans attracting increasing attention both as disease biomarkers targets for novel therapeutic approaches. Using a recently developed high-throughput high-performance liquid chromatography (HPLC) analysis method, we have reported, pilot genome-wide association study 13 glycan...
Aberrant glycosylation has been associated with a number of diseases including cancer. Our aim was to elucidate changes in whole plasma N-glycosylation between colorectal cancer (CRC) cases and controls one the largest cohorts its kind. A set 633 CRC patients 478 age gender matched analysed. Additionally, were stratified into four stages. Moreover, N-glycan analysis carried out 40 collected prior initial diagnosis CRC. Statistically significant differences observed N-glycome at all stages...
Abstract While aberrant protein glycosylation is a recognized characteristic of human cancers, advances in glycoanalytics continue to discover new associations between glycoproteins and tumorigenesis. This glycomics‐centric study investigates possible link paucimannosylation, an under‐studied class N ‐glycosylation [Man 1‐3 GlcNAc 2 Fuc 0‐1 ], cancer. The paucimannosidic glycans (PMGs) 34 cancer cell lines 133 tissue samples spanning 11 types matching non‐cancerous specimens are profiled...
The majority of proteins present in human serum/plasma are glycoproteins, validating this fluid as an ideal starting material for N-glycan analysis and discovery potential biomarkers. glycoprotein content both serum plasma is very similar, except removed the coagulation process, including fibrinogen. Our aim was to characterize fibrinogen glycosylation order determine its contribution differences between N-glycomes. N-Glycans from were released, labeled, analyzed by HILIC-HPLC MS. Structural...
Objective. Glycosylation is the most common post-translational modification and altered in disease. The typical glycosylation change patients with inflammatory arthritis (IA) a decrease galactosylation levels on IgG. aim of this study to evaluate effect anti-TNF therapy whole serum from IA determine whether these alterations glycome upon treatment
Galactose intoxication and over-restriction in galactosemia may affect glycosylation pathways cause multisystem effects. In this study, we describe an applied hydrophilic interaction chromatography ultra-performance liquid high-throughput method to analyze whole serum extracted IgG N-glycans with measurement of agalactosylated (G0), monogalactosylated (G1), digalactosylated (G2) structures as a quantitative measure galactose incorporation. This was nine children severe (genotype Q188R/Q188R)...
Complex carbohydrates are rapidly becoming excellent biomarker candidates because of their high sensitivity to pathological changes. However, the discovery clinical glycobiomarkers has been slow, due scarcity high-throughput glycoanalytical workflows that allow rapid glycoprofiling large sample sets. To generate high-quality quantitative glycomics data in a fashion, we have developed robotized platform for serum-based N-glycan preparation. The preparation workflow features fully automated,...
Helicobacter suis colonizes the stomach of most pigs and is prevalent non-Helicobacter pylori species found in human stomach. In host, H. contributes to development chronic gastritis, peptic ulcer disease MALT lymphoma, whereas it associated with decreased growth ulcers. Here, we demonstrate that level binding pig gastric mucins varies between individuals dependent specificity. The optimum at neutral pH has an acidic optimum, bind are different than those to. Mass spectrometric analysis...
Helicobacter suis is the most prevalent non-Helicobacter pylori species in human stomach and associated with chronic gastritis, peptic ulcer disease, gastric mucosa-associated lymphoid tissue (MALT) lymphoma. H. colonizes mucosa of 60-95% pigs at slaughter age, decreased weight gain, ulcers. Here, we show that experimental infection changes mucin composition glycosylation, decreasing amount suis-binding glycan structures pig mucus niche. Similarly, ability mucins from pylori-infected humans...
Glycosylation is highly variable depending on many environmental factors. Using our fully quantitative high-throughput normal phase hydrophilic interaction liquid chromatography platform we have identified glycosylation changes associated with medication in the plasma N-glycome from three different population cohorts: ORCADES Orkney Islands Scotland and CROATIA-Vis CROATIA-Korcula Croatian islands of Vis Korcula. Associations between use hormones (oral contraceptives, hormone replacement...
Juvenile idiopathic arthritis (JIA) encompasses all forms of chronic that arise before age 16. Previous studies have found JIA to be associated with lower Fc galactosylation circulating IgG, but the overall spectrum glycan changes and net impact on IgG function are unknown. Using ultra performance liquid chromatography (UPLC), we compared glycosylation in 54 subjects recent-onset untreated 98 healthy pediatric controls, paired biophysical profiling affinity for 20 receptors using a...
Rheumatoid arthritis is associated with an excess of agalactosylated (G0) IgG that considered relatively proinflammatory. Assessment this association in juvenile idiopathic (JIA) complicated by age-dependent glycan variation. The aim study was to conduct the first large-scale survey glycans healthy children and patients JIA, a focus on early childhood, time peak JIA incidence.IgG from disease-modifying antirheumatic drug-naive were characterized using high-performance liquid chromatography....