A5075818644- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Proteoglycans and glycosaminoglycans research
- Monoclonal and Polyclonal Antibodies Research
- Silk-based biomaterials and applications
- Galectins and Cancer Biology
- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Studies on Chitinases and Chitosanases
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Biochemical and Structural Characterization
- ATP Synthase and ATPases Research
- Pancreatic function and diabetes
- Protein Interaction Studies and Fluorescence Analysis
- Angiogenesis and VEGF in Cancer
- Retinal Development and Disorders
- Protein purification and stability
- Botulinum Toxin and Related Neurological Disorders
- Microbial Metabolic Engineering and Bioproduction
- Connective tissue disorders research
- Mitochondrial Function and Pathology
- Nanoparticle-Based Drug Delivery
- Immunotherapy and Immune Responses
ARC Centre of Excellence in Synthetic Biology
2022-2025
Macquarie University
2015-2025
Advances in software-driven glycopeptide identification have facilitated N-glycoproteomics studies reporting thousands of intact N-glycopeptides, i.e., N-glycan-conjugated peptides, but the automated process remains to be scrutinized. Herein, we compare site-specific glycoprofiling efficiency PTM-centric search engine Byonic relative manual expert annotation utilizing typical glycoproteomics acquisition and data analysis strategies with a single glycoprotein, uncharacterized multiple...
Metabolic diseases such as obesity and type 2 diabetes are marked by insulin resistance
Abstract While aberrant protein glycosylation is a recognized characteristic of human cancers, advances in glycoanalytics continue to discover new associations between glycoproteins and tumorigenesis. This glycomics‐centric study investigates possible link paucimannosylation, an under‐studied class N ‐glycosylation [Man 1‐3 GlcNAc 2 Fuc 0‐1 ], cancer. The paucimannosidic glycans (PMGs) 34 cancer cell lines 133 tissue samples spanning 11 types matching non‐cancerous specimens are profiled...
The mammalian glycome is structurally complex and diverse, composed of many glycan classes such as N- O-linked glycans, glycosaminoglycans (GAGs), glycosphingolipids (GSLs), other distinct features polysialic acids (PolySia), sulfation, proteoglycan attachment stubs. Various methods are used to analyze these different components the glycome, but they require prefractionated/partitioned samples target each class individually. To address this need for a knowledge relationship between...
Surface-enhanced Raman scattering (SERS) nanotags functionalized with lectins as the biological recognition element can be used to target carbohydrate portion of carbohydrate-carrying molecules (glycoconjugates).
Human soluble CD52 is a short glycopeptide comprising 12 amino acids (GQNDTSQTSSPS) which functions as an immune regulator by sequestering the pro-inflammatory high mobility group box protein 1 (HMGB1) and supressing responses. Recombinant has been shown to act broad anti-inflammatory agent, dampening both adaptive innate This heavily glycosylated, with complex sialylated N-linked glycan at N3 reported O-linked glycosylation possible on several serine threonine residues. Previously we...
Glycosylation is known to play an important role in IgG antibody structure and function. Polymeric IgM, the largest humans, displays five potential N-glycosylation sites on each heavy chain monomer. IgM can exist as a pentamer with connecting singly N-glycosylated J-chain (with total of 51 glycosylation sites) or hexamer (60 sites). In this study, recombinant pentameric hexameric produced by same human cell type culture conditions was site-specifically profiled RP-LC-CID/ETD-MS/MS using...
Growth factors are key molecules involved in angiogenesis, a process critical for tissue repair and regeneration. Despite the potential of growth factor delivery to stimulate limited clinical success has been achieved with this approach. interact extracellular matrix (ECM), particularly heparan sulphate (HS), bind potentiate their signalling. Here we show that engineered short forms perlecan, major HS proteoglycan vascular ECM, signal angiogenic factors, including fibroblast 2 endothelial...
Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking. Altered profiles are associated with cancer inflammatory diseases, well impacting efficacy of therapeutic monoclonal antibodies. Consisting oligosaccharides attached to asparagine residues, enzymatically released N- linked glycans analytically challenging due diversity isomeric structures exist. A commonly used technique for quantitative...
Precise characterization of a tissue's extracellular matrix (ECM) protein composition (matrisome) is essential for biomedicine. However, ECM extraction that requires organ-specific optimization still major limiting factor in matrisome studies. In particular, the mouse kidneys understudied, despite models being crucial renal research. Here, we comprehensively characterized healthy C57BL/6 mice using two methods combination with liquid chromatography tandem mass spectrometry (LC-MS/MS),...
The analysis of the structures glycans present on glycoproteins is an essential component for determining glycoprotein function; however, detailed glycan structural assignment glycopeptides from proteomics mass spectrometric data remains challenging. Glycoproteomic by spectrometry currently can provide significant, yet incomplete, information about present, including monosaccharide composition and in some circumstances site(s) glycosylation. Advancements resolution, using high-mass accuracy...
Wastewater from processing crustacean shell features ultrahigh chloride content. Bioremediation of the wastewater is challenging due to high ion content, making it inhospitable for most microorganisms survive and growth. In this study, mangrove wetland-derived fungi were first tested their salt tolerance, highly tolerant isolates cultured in shrimp concentration was monitored. Notably, filamentous fungal species Aspergillus piperis could remove over 70% within 3 days, with fastest biomass...
Abstract Opioid use for treatment of persistent pain has increased dramatically over the past two decades, but it not resulted in improved management outcomes. To understand molecular mechanisms opioids, signatures that arise from opioid exposure are often sought after, using various analytical methods. In this study, we performed proteomics, and multiglycomics via sequential analysis polysialic acids, glycosaminoglycans, N-glycans O-glycans, same cerebral spinal fluid (CSF) sample patients...
Skeletal dysplasias are a group of rare genetic disorders that affect growth and development the skeleton, leading to physical deformities other medical problems. High-throughput genome sequencing technologies have made it easier genotype disorder, but do not always reflect phenotypic outcome. CHST3-related skeletal dysplasia is caused by reduced function carbohydrate sulfotransferase sulfates chondroitin sulfate glycosaminoglycans. We show in this pilot study we were able phenotype patients...
Abstract Synthetic vascular grafts are used to bypass significant arterial blockage when native blood vessels unsuitable, yet their propensity fail due poor compatibility and progressive graft stenosis remains an intractable challenge. Perlecan is the major heparan sulfate (HS) proteoglycan in vessel wall with inherent ability regulate cell activities associated these failure modes. Here of engineered form perlecan domain V (rDV) bind angiogenic growth factors tuned endothelial proliferation...
Human soluble CD52 is a short glycopeptide comprising 12 amino acids (GQNDTSQTSSPS) which functions as an immune regulator by sequestering the pro-inflammatory high mobility group box protein 1 (HMGB1) and supressing responses. Recombinant has been shown to act broad anti-inflammatory agent, dampening both adaptive innate This heavily glycosylated, with complex sialylated N-linked glycan at N3 reported O-linked glycosylation possible on several serine threonine residues. Previously we...