A5085518723- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Extracellular vesicles in disease
- Ubiquitin and proteasome pathways
- Galectins and Cancer Biology
- Cancer, Hypoxia, and Metabolism
- S100 Proteins and Annexins
- Carbohydrate Chemistry and Synthesis
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Cancer Research and Treatments
- Genomics and Chromatin Dynamics
- Metabolism, Diabetes, and Cancer
- Ferroptosis and cancer prognosis
- Retinal Development and Disorders
- Calpain Protease Function and Regulation
- Colorectal Cancer Treatments and Studies
- Protease and Inhibitor Mechanisms
- Immune cells in cancer
- Neurotransmitter Receptor Influence on Behavior
- Fibroblast Growth Factor Research
- Neuropeptides and Animal Physiology
- 14-3-3 protein interactions
Boston University
2016-2024
Macquarie University
2012-2019
Abstract Introduction Extracellular vesicles (EVs) from human Alzheimer's disease (AD) biospecimens contain amyloid beta (Aβ) peptide and tau. While AD EVs are known to affect brain pathobiology, their biochemical molecular characterizations remain ill defined. Methods were isolated the cortical gray matter of 20 18 control brains. Tau Aβ levels measured by immunoassay. Differentially expressed EV proteins assessed quantitative proteomics machine learning. Results Levels pS396 tau Aβ1–42...
Advances in colorectal cancer (CRC) diagnosis will be enhanced by development of more sensitive and reliable methods for early detection the disease when treatment is effective. Because many known biomarkers are membrane-bound glycoproteins with important biological functions, we chose to compare N-glycan profiles membrane proteins from three phenotypically different CRC cell lines, LIM1215, LIM1899, LIM2405, representing moderately differentiated metastatic, primary, poorly (aggressive)...
Glycomics may assist in uncovering the structure–function relationships of protein glycosylation and identify glycoprotein markers colorectal cancer (CRC) research. Herein, we performed label-free quantitative glycomics on a carbon-liquid chromatography–tandem mass spectrometry-based analytical platform to accurately profile N-glycosylation changes associated with CRC malignancy. N-Glycome profiling was isolated membrane proteomes paired tumorigenic adjacent non-tumorigenic colon tissues...
Abstract While aberrant protein glycosylation is a recognized characteristic of human cancers, advances in glycoanalytics continue to discover new associations between glycoproteins and tumorigenesis. This glycomics‐centric study investigates possible link paucimannosylation, an under‐studied class N ‐glycosylation [Man 1‐3 GlcNAc 2 Fuc 0‐1 ], cancer. The paucimannosidic glycans (PMGs) 34 cancer cell lines 133 tissue samples spanning 11 types matching non‐cancerous specimens are profiled...
Glioblastoma (GBM) is the most common and malignant primary brain tumor. The extracellular matrix, also known as matrisome, helps determine glioma invasion, adhesion, growth. Little attention, however, has been paid to glycosylation of matrix components that constitute majority glycosylated protein mass presumed biological properties. To acquire a comprehensive understanding functions matrisome its components, including proteoglycans (PGs) glycosaminoglycans (GAGs), in GBM tumorigenesis,...
Recent findings show that effective integration of novel information in the brain requires coordinated processes homo- and heterosynaptic plasticity. In this work, we hypothesize activity-dependent remodeling peri-synaptic extracellular matrix (ECM) contributes to these processes. We clusters ECM, recognized by CS56 antibody, emerge response sensory stimuli, showing temporal spatial coincidence with dendritic spine Using co-immunoprecipitation synaptosomal proteins, identify several...
We describe a protocol for glycomic and proteomic profiling that uses serial enzyme digestions from the surface of fresh frozen or fixed tissue slides. The abundances extracted glycans peptides are determined using liquid chromatography-mass spectrometry. In typical experiment, our method quantifies 14 heparan sulfate disaccharides, 11 chondroitin 50 N-glycan compositions approximately 1200 proteins 1.8 mm circle, on slide rat brain. Each enzymatic digestion is incubated overnight with...
Medulloblastoma (MB) is the most common malignant pediatric brain tumor. Patient survival has remained largely same for past 20 years, with therapies causing significant health, cognitive, behavioral and developmental complications those who survive In this study, we profiled total transcriptome proteome of two established MB cell lines, Daoy UW228, using high-throughput RNA sequencing (RNA-Seq) label-free nano-LC-MS/MS-based quantitative proteomics, coupled advanced pathway analysis. While...
A high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis.
Abstract Substance abuse is a pressing problem with few therapeutic options. The identification of addiction resilience factors potential strategy to identify new mechanisms that can be targeted therapeutically. Heparan sulfate (HS) linear sulfated polysaccharide component the cell surface and extracellular matrix. modulates activity distribution set negatively charged signaling peptides proteins — known as HS interactome by acting co-receptor or alternative receptor for growth other...
In this manuscript, we describe a shotgun proteomics approach for comprehensive proteomic analysis of samples including total lysates, membrane, secretome, and exosome fractions from panel colorectal cancer cell lines. We will present an our data in two alternative formats. First discuss traditional data, which identify number cancer-associated proteins using various tools. second approach, use chromosome format to organize the on 7, allowing identification clusters genes with boundaries...
Abstract Introduction Extracellular vesicles (EVs) from human Alzheimer’s disease (AD) biospecimens contain amyloid-β peptide (Aβ) and tau. While AD EVs are known to affect brain pathobiology, their biochemical molecular characterizations remain ill defined. Methods were isolated the cortical grey matter of 20 18 control brains. Tau Aβ levels measured by immunoassay. Differentially expressed EV proteins assessed quantitative proteomics machine learning. Results Levels pS396 tau significantly...
V-erb-b2 erythroblastic leukemia viral oncogene homologue 2, known as ERBB2, is an important in the development of certain cancers. It can form a heterodimer with other epidermal growth factor receptor family members and activate kinase-mediated downstream signaling pathways. ERBB2 gene located on chromosome 17 amplified subset cancers, such breast, gastric, colon cancer. Of particular interest to Chromosome-Centric Human Proteome Project (C-HPP) initiative amplification mechanism that...