A5060437129- Extracellular vesicles in disease
- Cardiovascular Disease and Adiposity
- Circular RNAs in diseases
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Pulmonary Hypertension Research and Treatments
- Angiogenesis and VEGF in Cancer
- Systemic Sclerosis and Related Diseases
- Congenital heart defects research
- T-cell and B-cell Immunology
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Mesenchymal stem cell research
- Lymphatic System and Diseases
- Immunodeficiency and Autoimmune Disorders
- Hearing, Cochlea, Tinnitus, Genetics
- Autophagy in Disease and Therapy
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Electrospun Nanofibers in Biomedical Applications
- Metabolism, Diabetes, and Cancer
- Diabetes and associated disorders
- Biomarkers in Disease Mechanisms
- Sulfur Compounds in Biology
- Cardiovascular Function and Risk Factors
Temple University
2016-2025
Benedict College
2015-2023
University of Alabama at Birmingham
1997-2017
Indianapolis Zoo
2012-2017
Louisiana State University Health Sciences Center New Orleans
2016
Northwestern University
2015
Case Western Reserve University
2009-2012
Zionsville Community High School
2012
Princeton University
2000
Rationale: Embryonic stem cells (ESCs) hold great promise for cardiac regeneration but are susceptible to various concerns. Recently, salutary effects of have been connected exosome secretion. ESCs the ability produce exosomes, however, their effect in context heart is unknown. Objective: Determine ESC-derived repair ischemic myocardium and whether c-kit + progenitor (CPCs) function can be enhanced with ESC exosomes. Methods Results: This study demonstrates that mouse exosomes (mES Ex)...
Abstract Circular RNAs are generated from many protein-coding genes, but their role in cardiovascular health and disease states remains unknown. Here we report identification of circRNA transcripts that differentially expressed post myocardial infarction (MI) mouse hearts including circFndc3b which is significantly down-regulated the post-MI hearts. Notably, human ortholog also cardiac tissues ischemic cardiomyopathy patients. Overexpression endothelial cells increases vascular growth...
Rationale: Systemic inflammation compromises the reparative properties of endothelial progenitor cell (EPC) and their exosomes on myocardial repair, although underlying mechanism loss function from inflamed EPCs is still obscure. Objective: To determine mechanisms IL-10 (interleukin-10) deficient-EPC–derived exosome dysfunction in repair to investigate if modification specific cargo can rescue activity. Methods Results: Using knockout mice mimicking systemic condition, we compared...
Increased miR-375 levels has been implicated in rodent models of myocardial infarction (MI) and with patients heart failure. However, no prior study had established a therapeutic role ischemic myocardium. Therefore, we assessed whether inhibition MI-induced by LNA anti-miR-375 can improve recovery after acute MI. Ten weeks old mice were treated either control or anti induction MI LAD ligation. The inflammatory response, cardiomyocyte apoptosis, capillary density left ventricular (LV)...
Summary: In mice, the absence of terminal deoxynucleotidyl transferase (Tdt) expression during fetal and neonatal life provides a window in development where clones lymphocytes are generated that provide protective immunity. Introducing premature Tdt activity interferes with these results an impaired ability to make antibodies. Conversely, gene‐targeted disruption prevents N additions at all stages T B‐lymphocyte promotes fetal‐like B‐cell into adulthood, accompanying alterations repertoire....
Background: Activated fibroblasts (myofibroblasts) play a critical role in cardiac fibrosis; however, their origin the diseased heart remains unclear, warranting further investigation. Recent studies suggest contribution of bone marrow fibroblast progenitor cells (BM-FPCs) pressure overload–induced fibrosis. We have previously shown that interleukin-10 (IL10) suppresses IL10 inhibition BM-FPC–mediated fibrosis is not known. hypothesized inhibits homing BM-FPCs to and transdifferentiation...
Poor survival and function of transplanted cells in ischemic inflamed myocardium likely compromises the functional benefit stem cell-based therapies. We have earlier reported that co-administration interleukin (IL)-10 BMPAC enhances cell improves left ventricular (LV) functions after acute myocardial infarction (MI) mice. hypothesized IL-10 regulates microRNA-375 (miR-375) signaling BMPACs to enhance their MI attenuates dysfunction MI. miR-375 expression is significantly upregulated upon...
Background and Purpose: Myocardial infarction (MI) in diabetic patients results higher mortality morbidity. We others have previously shown that bone marrow-endothelial progenitor cells (EPCs) promote cardiac neovascularization attenuate ischemic injury. Lately, small extracellular vesicles (EVs) emerged as major paracrine effectors mediating the benefits of stem cell therapy. Modest clinical outcomes autologous cell-based therapies suggest diabetes-induced EPC dysfunction may also reflect...
Heart failure (HF) is one of the leading causes mortality worldwide. Extracellular vesicles, including small extracellular vesicles or exosomes, and their molecular cargo are known to modulate cell-to-cell communication during multiple cardiac diseases. However, role systemic vesicle biogenesis inhibition in HF models not well documented remains unclear.
Bone marrow cell (BMC)-based treatment for critical limb ischemia in diabetic patients yielded a modest therapeutic effect resulting from dysfunction. Therefore, approaches that improve stem/progenitor functions may provide benefits. Here, we tested the hypothesis restoration of hydrogen sulfide (H2S) production BMCs improves their reparative capacities.Mouse were isolated by density-gradient centrifugation. Unilateral hind was conducted 12- to 14-week-old db/+ and db/db mice ligation left...
Background: Mechanisms of macrophage switching from pro-inflammatory to anti-inflammatory phenotypes are not well understood. Circular RNAs (circRNAs), a new class non-coding RNAs, implicated in immune modulation. We recently identified circ-cdr1as as regulator phenotype bone marrow derived macrophages (BMDM), however, their role immunomodulation during cardiovascular injury remains unknown. Methods: Cell-specific expression levels was determined mouse hearts post-myocardial infarction (MI)....
Cardiac amyloidosis is a secondary phenomenon of an already pre-existing chronic condition. Whether cardiac represents one the complications post myocardial infarction (MI) has yet to be fully understood. Here, we show that occurs after MI and amyloid fibers are composed macrophage-derived serum A 3 (SAA3) monomers. SAA3 overproduction in macrophages triggered by exosomal communication from stromal cells (CSCs), which, response MI, activate expression platelet aggregation-inducing type I...
Mutations in OTOF gene, encoding otoferlin, cause DFNB9 deafness and non-syndromic auditory neuropathy (AN). The aim of this study is to identify mutations Chinese patients with neuropathy.73 unrelated Han AN, including one case temperature sensitive (TS-NSRAN) 92 ethnicity-matched controls normal hearing were screened. Forty-five pairs PCR primers designed amplify all the exons their flanking regions gene. products sequenced analyzed for mutation identification.Five novel possibly...
Abstract Historically, a lower incidence of cardiovascular diseases (CVD) and related deaths in women as compared with men the same age has been attributed to female sex hormones, particularly estrogen its receptors. Autologous bone marrow stem cell (BMSC) clinical trials for cardiac therapy overwhelmingly included male patients. However, meta-analysis data from these suggest better functional outcome postmenopausal aged-matched men. Mechanisms governing sex-specific reparative activity...
Streptococcus pneumoniae is the most common pathogen associated with otitis media. To examine role of Toll-like receptor 2 (TLR2) in host defense against infection middle ear, wild-type (WT; C57BL/6) and TLR2-deficient (TLR2(-/-)) mice were inoculated (1 x 10(6) CFU) through tympanic membrane. Nineteen 37 TLR2(-/-) showed bacteremia died within 3 days after challenge, compared to only 4 32 WT that died. Of those survived, more severe hearing loss than was indicated by an elevation...
Podoplanin, a small mucine-type transmembrane glycoprotein, has been recently shown to be expressed by lymphangiogenic, fibrogenic and mesenchymal progenitor cells in the acutely chronically infarcted myocardium. Podoplanin binds CLEC-2, C-type lectin-like receptor 2 highly CD11bhigh following inflammatory stimuli. Why podoplanin expression appears only after organ injury is currently unknown. Here, we characterize role of different stages myocardial repair infarction propose...
Genetic predisposition is recognized as an important pathogenetic factor in otitis media (OM) and associated diseases. Mutant Lmna mice heterozygous for the disheveled hair ears allele (LmnaDhe/+) exhibit early-onset, profound hearing deficits other pathological features mimicking human laminopathy with LMNA mutation. We assessed effects of LmnaDhe/+ mutation on development OM abnormalities characteristic laminopathy. Malformation abnormal positioning eustachian tube, accompanied by OM, were...
Macrophages are crucial for the initiation and resolution of an inflammatory response. Non-coding circular RNAs ubiquitously expressed in mammalian tissue, highly conserved among species, recently implicated regulation macrophage activation. We sought to determine whether circRNAs modulate monocyte/macrophage biology function.We performed circRNA microarray analyses assess transcriptome changes using RNA isolated from bone marrow derived macrophages polarized a pro-inflammatory phenotype...
Rare entities in the Pap test, including neoplastic and non-neoplastic conditions, pose challenges due to their infrequent occurrence daily practice of cytology. Furthermore, these conditions give rise important diagnostic pitfalls. Infections such as tuberculosis cervicitis may be erroneously diagnosed carcinoma, whereas others, schistosomiasis, are associated with squamous cell carcinoma. These cases include granuloma inguinale (donovanosis), tuberculosis, coccidioidomycosis, taeniasis,...
Conclusion: GJB2 mutation was frequent in sporadic outpatients and its frequency significant higher the prelingual group than postlingual group, whereas of mtDNA A1555G SLC26A4 very rare Chinese with nonsyndromic sensorineural hearing loss (NSHL). Standard comprehensive inclusion grouping criteria are necessary for epidemiological studies deafness-related gene mutations. Objectives: This study aimed to examine mutations three common deafness genes GJB2, SLC26A4, NSHL discuss factors that...