A5015718586- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Immunodeficiency and Autoimmune Disorders
- Genetic and phenotypic traits in livestock
- Immune Response and Inflammation
- Genetic Mapping and Diversity in Plants and Animals
- Diabetes and associated disorders
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Cell Adhesion Molecules Research
- Galectins and Cancer Biology
- Blood groups and transfusion
- Reproductive Physiology in Livestock
- Bacterial Genetics and Biotechnology
- Chronic Lymphocytic Leukemia Research
- Peptidase Inhibition and Analysis
- Pediatric health and respiratory diseases
- Atherosclerosis and Cardiovascular Diseases
- Antimicrobial Peptides and Activities
- Cancer-related molecular mechanisms research
- Protein purification and stability
- CAR-T cell therapy research
University of Alabama at Birmingham
2015-2024
Technological University Dublin
2024
King's College - North Carolina
2020
King's College London
2020
University of Washington
2020
Curtin University
2019
Action Network
2019
Research Network (United States)
2019
University of East Anglia
2019
University of Wisconsin–Madison
2019
Abstract We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid lines producing pure monoclonal antibodies. Screening specificity and classes was done modified enzyme-linked immunosorbent assay.
A rat monoclonal antibody specific for immunoglobulin (Ig) heavy chain binding protein (BiP) has allowed the examination of association BiP with assembling Ig precursors in mouse B lymphocyte-derived cell lines. The anti-BiP immunoprecipitates along noncovalently associated chains. is a component endoplasmic reticulum and binds free intracellular chains nonsecreting pre-B (mu+, L-) lines or incompletely assembled (H+, L+) secreting hybridomas myelomas. In absence light synthesis, remain are...
The antiphosphocholine (PC) antibody in normal mouse sera (NMS) provides protection against intravenous infection with encapsulated strain WU2 of type 3 Streptococcus pneumoniae. Mice unable to make anti-PC antibody, as a result suppression anti-T-15 idiotype or inheritance the xid gene CAB/N mice, are highly susceptible WU2. inheriting can be protected NMS from immunologically mice IgM hybridoma antibody. protective effect removed PC-containing immunoabsorbents.
Abstract Mouse splenic B cells can be separated based on their distinctive expression of cell surface antigens. Marginal zone (MZ) are CD38 high CD21 CD23 low/− , while follicular (FO) int and newly formed (NF) dim/− − . Exploiting these phenotypic distinctions, we isolated the three subsets assessed other differences functional capabilities in vitro. FO proliferate more than response to either IgM or cross‐linking. MZ better when stimulated with lipopolysaccharide (LPS), sub‐optimal levels...
Although antibody diversity arises mainly from apparently random combinatorial and somatic mutational mechanisms acting upon a limited number of germline genes, the repertoire develops in an ordered fashion during mammalian ontogeny. A series early pre-B B-lymphocyte cell lines were examined to determine whether rearrangement gene families variable region immunoglobulin heavy chains (VH) may be basis for programmed development response. The results indicated that VH fetal B-lineage cells is...
Heavy chain-binding protein (BiP) associates posttranslationally with nascent Ig heavy chains in the endoplasmic reticulum (ER) and remains associated these until they assemble light chains. The chain-BiP complex can be precipitated by antibody reagents against either component. To identify sites on chain molecules that are important for association BiP, we have examined 30 mouse myelomas hybridomas synthesize well characterized deletions. Mutant lack CH1 domain could not demonstrated to...
Summary: The rate of elimination a pathogenic agent is critical importance for the host and determines extent consequences infection. Antibody production, along with activity other cells immune system, plays an important role early late in response contributes to all containment organism. B‐cell clones reaching mature long‐lived pool are heterogeneous: some belong B1 subset, enriched CD21 high compartment (mostly marginal zone (MZ)), whereas others recirculate primarily among follicles (FO)....
Ag encounter will recruit Ag-specific cells from the pool of mature B lymphocytes in spleen and activate them to perform effector functions: generation Ab-forming (plasma cells) presentation T cells. We have compared ability follicular marginal zone develop into The their localization sinus area are cell CD40 ligand independent, suggesting that they do not represent a postgerminal center population. Compared with recirculating cells, express several characteristics previous antigenic...
Abstract The early involvement of marginal zone (MZ) B lymphocytes in T-independent immune responses is well established. In this study we compared the abilities MZ and follicular (FO) cells to collaborate with T cells. After immunization soluble hen egg lysozyme, both FO captured Ag migrated cell areas response lysozyme. were far superior inducing CD4+ expansion vitro vivo. MZ, but not FO, cells, after interaction differentiated into plasma addition they stimulated Ag-specific produce high...
Spores of Bacillus anthracis, the causative agent anthrax, are enclosed by a prominent loose-fitting, balloon-like layer called exosporium. Although exosporium serves as source surface antigens and primary permeability barrier spore, its molecular structure function not well characterized. In this study, we identified five major proteins in purified B. anthracis (Sterne strain) exosporia. One protein was recently collagen-like glycoprotein BclA, which appears to be structural component...