A5077187844- Circular RNAs in diseases
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Histone Deacetylase Inhibitors Research
- Glutathione Transferases and Polymorphisms
- RNA modifications and cancer
- Biochemical effects in animals
- Aldose Reductase and Taurine
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Advanced Surface Polishing Techniques
- Advanced machining processes and optimization
- 14-3-3 protein interactions
- Genomics and Chromatin Dynamics
- RNA regulation and disease
- Cancer Cells and Metastasis
- Electromagnetic wave absorption materials
- CRISPR and Genetic Engineering
- Cardiovascular Disease and Adiposity
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Metallurgy and Material Forming
- RNA Interference and Gene Delivery
- Thermal Radiation and Cooling Technologies
Beijing Technology and Business University
2022-2024
Sun Yat-sen University
2024
Sun Yat-sen Memorial Hospital
2024
University of Pennsylvania
2014-2023
Shanghai Liangyou (China)
2017-2018
Philadelphia University
2012-2015
Drexel University
2012-2015
Oslo University Hospital
2011-2013
University of Oslo
2011-2013
Thomas Jefferson University
2006-2010
Recent deep sequencing studies have revealed thousands of circular noncoding RNAs generated from protein-coding genes. These are produced when the precursor messenger RNA (pre-mRNA) splicing machinery “backsplices” and covalently joins, for example, two ends a single exon. However, mechanism by which spliceosome selects only certain exons to circularize is largely unknown. Using extensive mutagenesis expression plasmids, we show that miniature introns containing splice sites along with short...
Thousands of eukaryotic protein-coding genes are noncanonically spliced to produce circular RNAs. Bioinformatics has indicated that long introns generally flank exons circularize in Drosophila , but the underlying mechanisms by which these RNAs generated largely unknown. Here, using extensive mutagenesis expression plasmids and RNAi screening, we reveal circularization laccase2 gene is regulated both intronic repeats trans -acting splicing factors. Analogous what been observed humans mice,...
Abstract Circular RNAs are generated from many protein-coding genes, but their role in cardiovascular health and disease states remains unknown. Here we report identification of circRNA transcripts that differentially expressed post myocardial infarction (MI) mouse hearts including circFndc3b which is significantly down-regulated the post-MI hearts. Notably, human ortholog also cardiac tissues ischemic cardiomyopathy patients. Overexpression endothelial cells increases vascular growth...
Circular RNAs (circRNAs) are generated from many protein-coding genes. Most accumulate in the cytoplasm, but how circRNA localization or nuclear export is controlled remains unclear. Using RNAi screening, we found that depletion of Drosophila DExH/D-box helicase Hel25E results accumulation long (>800-nucleotide), not short, circRNAs. The human homologs similarly regulate localization, as UAP56 (DDX39B) URH49 (DDX39A) causes and short circRNAs, respectively, to become enriched nucleus....
Cellular homeostasis requires transcriptional outputs to be coordinated, and many events post-transcription initiation can dictate the levels functions of mature transcripts. To systematically identify regulators inducible gene expression, we performed high-throughput RNAi screening Drosophila Metallothionein A (MtnA) promoter. This revealed that Integrator complex, which has a well-established role in 3′ end processing small nuclear RNAs (snRNAs), attenuates MtnA transcription during copper...
CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for detecting and depleting RNAs of interest. Near perfect complementarity between a target RNA the Cas13-associated guide is required activation Cas13 ribonuclease activity. Nonetheless, specificity in eukaryotic cells has been debated nuclease domains can be exposed on enzyme surface, providing potential promiscuous cleavage nearby (so-called collateral damage). Here, using co-transfection assays...
Adaptation to hypoxic microenvironment is critical for tumor survival and metastatic spread.Hypoxiainducible factor 1␣ (HIF-1␣) plays a key role in this adaptation by stimulating the production of proangiogenic factors inducing enzymes necessary anaerobic metabolism.Histone deacetylase inhibitors (HDACIs) produce marked inhibition HIF-1␣ expression are currently clinical trials partly based on their potent antiangiogenic effects.Although it has been postulated that HDACIs affect enhancing...
Hypoxia-inducible factor 1 (HIF-1) transcriptionally promotes production of adenosine triphosphate (ATP) whereas AMPK senses and regulates cellular energy homeostasis. A histone deacetylase (HDAC) activity has been proven to be critical for HIF-1 activation but the underlying mechanism its role in homesostasis remain unclear. Here, we demonstrate that depends on a cytosolic, enzymatically active HDAC5. HDAC5 knockdown impairs hypoxia-induced HIF-1α accumulation transactivation,...
Aberrant translation initiation at non-AUG start codons is associated with multiple cancers and neurodegenerative diseases. Nevertheless, how may be regulated differently from canonical poorly understood. Here, we used codon-specific reporters ribosome profiling to characterize responds protein synthesis inhibitors in human cells. These analyses surprisingly revealed that of non-AUG-encoded the endogenous GUG-encoded
Hypoxia-inducible factors (HIFs) are heterodimeric transcription regulating the oxygen supply, glucose metabolism, and angiogenesis. HIF function requires recruitment of p300/CREB-binding protein, two coactivators with histone acetyltransferase activity, by C-terminal transactivation domain HIF-alpha (HIF-alphaCAD). Histone deacetylase inhibitors (HDAIs) induce differentiation or apoptosis repress tumor growth angiogenesis, hence being explored intensively as anti-cancer agents. Using...
Hypoxia is an important environmental change in many cancers. Hypoxic niches can be occupied by cancer stem/progenitor-like cells that are associated with tumor progression and resistance to radiotherapy chemotherapy. However, it has not yet been fully elucidated how hypoxia influences the stem-like properties of prostate cells. In this report, we investigated effects on human cell lines, PC-3 DU145. comparison normoxia (20% O2), 7% O2 induced higher expressions HIF-1α HIF-2α, which were...
Abstract Background To study whether hypoxia influences the stem-like properties of ovarian cancer cells and their biological behavior under hypoxia. Method Ovarian cell lines ES-2 OVCAR-3 were cultivated in different oxygen tensions for proliferation, cycling invasion analyses. The clonogenic potential was examined by colony formation sphere assays. Stem surface markers, SP CD44 bright dim analyzed flow cytometry. Protein expression HIF-1α, HIF-2α, Ot3/4 Sox2 investigated Western blotting....
Glutaminolysis and Warburg effect are the two most noticeable metabolic features of tumor cells whereas their biological significance in cell proliferation remains elusive. A widely accepted current hypothesis is that use glutamine as a preferred carbon source for energy reducing power, which has been used to explain both glutaminolysis effect. Here we provide evidence show supplying nitrogen, not skeleton, underlies major importance proliferating cells. We alternative nitrogen mechanisms...
Abstract p300/cyclic AMP–responsive element binding protein–binding protein (CBP) are general coactivators for multiple transcription factors involved in various cellular processes. Several highly conserved domains of p300/CBP serve as interacting sites and regulatory proteins. Particularly, the intrinsic histone acetyltransferase (HAT) activity transactivation (TAD) play essential roles their coactivating function. Autoacetylation is commonly observed cell-free HAT assays has been...
Glutamine (Gln) and glucose (Glc) represent two important nutrients for proliferating cells, consistent with the observations that oncogenic processes are associated enhanced glycolysis glutaminolysis. Gln depletion Glc have been shown to trigger growth arrest eventually cell death. Solid tumors often outgrow blood supply, resulting in ischemia, which is hypoxia nutrient insufficiency. Whereas oxygen-sensing adaptive mechanisms well-studied, how cells directly sense respond insufficiency...
Notch signaling involves the processes that govern cell proliferation, fate decision, differentiation and stem maintenance. Due to its fundamental role in cells, it has been speculated during recent years family may have critical functions cancer cells or with a phenotype, therefore playing an important process of oncogenesis. In this study, expression KYSE70, KYSE140 KYSE450 squamous esophageal lines virus transformed epithelial line Het-1A was examined by quantitative RT-PCR. Compared...
Bone marrow metastases are formed in the late phases of prostate cancer disease. Stem cell factor (SCF) and granulocyte colony-stimulating (G-CSF) present microenvironment bone play a vital role biology therein. The study was to investigate influence SCF G-CSF on stem-like properties lines. Upon stimulation with or G-CSF, higher levels CD117, ABCG2, CD44 were observed PC-3 DU145 cells examined by flow cytometry. Simultaneously, expressions Oct3/4 Nanog upregulated. Moreover, quantitative...
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Intracranial aneurysm is common in stroke and, once rupturing, will cause disaster to patients. Nowadays, endovascular treatment has become a routine reduce the risk of intracranial aneurysms rupture. Successive methods, like balloon-assisted coiling, stent-assisted and flow diversion, have new choices for doctors. More more doctors been entering this field. Understanding current general situation crucial medical workers learn aneurysms. In past, many devices ideas about appeared. Although...