A5007835296- Click Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
- Ion Transport and Channel Regulation
- Hormonal Regulation and Hypertension
- Molecular Sensors and Ion Detection
- Nitric Oxide and Endothelin Effects
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Sulfur Compounds in Biology
- Heme Oxygenase-1 and Carbon Monoxide
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Chemical Synthesis and Analysis
- Cancer-related gene regulation
- Porphyrin Metabolism and Disorders
- Mitochondrial Function and Pathology
- Bacterial Genetics and Biotechnology
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Heat shock proteins research
- Extracellular vesicles in disease
- Antimicrobial Peptides and Activities
University of California, Berkeley
2019-2025
Shenzhen Institutes of Advanced Technology
2025
China National Nuclear Corporation
2021-2024
Chengdu Medical College
2021-2024
QB3
2024
South China Agricultural University
2024
National Health and Family Planning Commission
2024
Xi'an Jiaotong University
2024
Institute of Industrial Economics
2022-2023
Jiangsu University
2022-2023
Site-selective bioconjugation to native protein residues is a powerful tool for functionalization, with cysteine and lysine side chains being the most common points attachment owing their high nucleophilicity. We now report strategy histidine modification using thiophosphorodichloridate reagents that mimic post-translational phosphorylation, enabling fast selective labeling of histidines under mild conditions where various payloads can be introduced via copper-assisted alkyne–azide...
Hypoxia-inducible factor 1 (HIF-1) transcriptionally promotes production of adenosine triphosphate (ATP) whereas AMPK senses and regulates cellular energy homeostasis. A histone deacetylase (HDAC) activity has been proven to be critical for HIF-1 activation but the underlying mechanism its role in homesostasis remain unclear. Here, we demonstrate that depends on a cytosolic, enzymatically active HDAC5. HDAC5 knockdown impairs hypoxia-induced HIF-1α accumulation transactivation,...
Abstract Coupling photocrosslinking reagents with mass spectrometry has become a powerful tool for studying protein–protein interactions in living systems, but it still suffers from high rates of false-positive identifications as well the lack information on interaction interface due to challenges deciphering crosslinking peptides. Here we develop genetically encoded photo-affinity unnatural amino acid that introduces spectrometry-identifiable label (MS-label) captured prey proteins after...
The AlkB family of nonheme Fe(II)/2-oxoglutarate-dependent oxygenases are essential regulators RNA epigenetics by serving as erasers one-carbon marks on with release formaldehyde (FA). Two major human members, FTO and ALKBH5, both act oxidative demethylases N6-methyladenosine (m6A) but furnish different products, N6-hydroxymethyladenosine (hm6A) adenosine (A), respectively. Here we identify foundational mechanistic differences between ALKBH5 that promote these distinct biochemical outcomes....
We have developed a genetically encoded, selenium-based cleavable photo-cross-linker that allows for the separation of bait and prey proteins after protein photo-cross-linking. further demonstrated efficient capture in situ generated selenenic acid on cleaved proteins. Our strategy involves tagging with an alkyne-containing dimethoxyaniline molecule subsequently labeling azide-bearing fluorophore or biotin probe. This cleavage-and-capture photo-cross-linking are readily accessible by...
http://isyslab.info/StraPep.
Abstract A genetically encoded, multifunctional photocrosslinker was developed for quantitative and comparative proteomics. By bearing a bioorthogonal handle releasable linker in addition to its photoaffinity warhead, this probe enables the enrichment of transient low‐abundance prey proteins after intracellular photocrosslinking prey–bait separation, which can be subject stable isotope dimethyl labeling mass spectrometry analysis. This strategy (termed isoCAPP) allowed proteomic approach...
Activity-based protein profiling (ABPP) is a versatile strategy for identifying and characterizing functional sites compounds therapeutic development. However, the vast majority of ABPP methods covalent drug discovery target highly nucleophilic amino acids such as cysteine or lysine. Here, we report methionine-directed platform using Redox-Activated Chemical Tagging (ReACT), which leverages biomimetic oxidative ligation selective methionine modification. Application ReACT to oncoprotein...
This study aimed to investigate the function and signaling pathway of Toll-like receptor 4 (TLR4) in oxidized low-density lipoprotein (oxLDL)-induced up-regulated expressions LDL 1 (LOX-1), monocyte chemoattractant protein (MCP-1), vascular cell adhesion molecule (VCAM-1) human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with different oxLDL concentrations (0, 20, 40, 60, 80 μg/mL) for 24 48 h. The influence on TLR4, LOX-1, MCP-1, VCAM-1 was detected by real-time...
Significance HdeA and HdeB are essential chaperones for many Gram-negative enteric bacteria to survive acid stress. How they cooperate protect a broad range of client proteins from denaturation while avoiding nonspecific binding remains elusive. Here we developed comparative proteomic strategy combining genetically encoded releasable photocross-linking with 2D difference gel electrophoresis, which allows an unbiased side-by-side comparison the entire pools these two acid-activated in...
Strengthening economic resilience is the key to stable growth in various regions. Previous studies have paid more attention level and evolution trend of resilience, seldom Dissected reasons for differences regional resilience. This paper takes 243 cities China as research object explains from perspective industrial diversification. The results show that diversification helps improve Compared with related diversification, unrelevant has a significant effect on region’s own endowment...
Fluorescence imaging the interplay between lipid droplets (LDs) and protein aggregates (PAs) is extremely valuable for elucidating molecular mechanisms of aging. Here, we describe first dual-functional fluorescent probe, LW-1, simultaneously LDs PAs in distinct fluorescence channels to dissect interplaying roles during Notably, based on an intriguing mechanism hydrogen bonds regulating single bond rotation, LW-1 selectively detected a red channel. Meanwhile, another intramolecular charge...
Background: Stanniocalcin 2 (STC2) expression is upregulated under multiple stress conditions including hypoxia, nutrient starvation and radiation. Overexpression of STC2 correlates with tumor progression poor prognosis. Purpose: We previously demonstrated that overexpression in nasopharyngeal carcinomas (NPC) positively radiation resistance metastasis, two major clinical obstacles to the improvement NPC management. However, it remains elusive whether a critical contributing factor for...