A5100375616- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Bone and Dental Protein Studies
- Wireless Communication Security Techniques
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Genomics and Phylogenetic Studies
- Cancer-related Molecular Pathways
- Bone Metabolism and Diseases
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Oral microbiology and periodontitis research
- Fuel Cells and Related Materials
- Coccidia and coccidiosis research
- Cancer-related molecular mechanisms research
- Advanced Image Fusion Techniques
- Veterinary medicine and infectious diseases
Northeast Forestry University
2025
Xinjiang Institute of Ecology and Geography
2024
Chinese Academy of Sciences
2015-2024
Heilongjiang Academy of Forestry
2024
Yantai University
2022-2024
Chongqing University
2022-2024
Xi'an Institute of Optics and Precision Mechanics
2022-2024
Institute of Physics
2015-2024
Songshan Lake Materials Laboratory
2022-2024
Capital Medical University
2012-2024
Abstract To identify novel biomarkers of metastasis colorectal cancer (CRC), we developed an orthotopic implantation model murine CRC and selected in vivo M5, a subclone the SW480 cell line with enhanced potential for to liver. We compared differences gene expression profiles between M5 cells using profiling. found that special AT‐rich sequence‐binding protein 2 (SATB2) was down‐regulated cells. Immunohistochemical analysis 146 tumour samples showed underexpression SATB2 strongly correlated...
Significance Transcription initiation by RNA polymerase (RNAP) is a highly regulated rate-limiting step in many genes and involves numerous intermediate states that remain incompletely understood. Here, we report the characterization of previously hypothesized slow pathway involving RNAP backtracking pausing. This backtracked paused state observed when all nucleoside triphosphates (NTPs) are present at physiologically relevant concentrations, but becomes more prevalent with unbalanced NTP...
Measurement of cardiac troponin I in the blood is crucial for early diagnosis acute myocardial infarction. Herein, a novel and ultrasensitive electrochemiluminescence (ECL) immunosensor has been developed determination (cTnI) by using Au nanoclusters hybridization chain reaction (HCR) signal amplification. In this ECL immunosensor, were dual-labeled at each end hairpin DNA (H1 H2) acted as luminophore. initiator strands (T1) secondary antibody (Ab2) conjugated on nanoparticles (AuNPs) to...
Abstract Programmed cell death 1 (PD-1) is inhibitory receptor and immune checkpoint protein. Blocking the interaction of PD-1 its ligands PD-L1/ L2 able to active T-cell-mediated antitumor response. Monoclonal antibody-based drugs targeting pathway have exhibited great promise in cancer therapy. Here we show that MW11-h317, an anti-PD-1 monoclonal antibody, displays high affinity for blocks interactions with PD-L1/L2. MW11-h317 can effectively induce response inhibit tumor growth mouse...
Acute kidney injury (AKI) is a clinical syndrome with high morbidity and mortality worldwide, there currently no effective means to prevent it. Dioscin naturally present in the dioscoreaceae plants has antioxidant anti-inflammatory effects. Here, we found that dioscin protective against cisplatin-induced AKI. Pathological ultrastructural observations revealed reduced renal tissue lesions mitochondrial damage. Furthermore, markedly suppressed reactive oxygen species malondialdehyde levels...
In kinesin-3, the coiled-coil 1 (CC1) can sequester preceding neck coil (NC) for autoinhibition, but underlying mechanism is poorly understood. Here, we determined structures of uninhibited motor domain (MD)-NC dimer and inhibited MD-NC-CC1 monomer kinesin-3 KIF13B. monomer, CC1 broken into two short helices that unexpectedly interact with both NC MD. Compared MD-NC dimer, CC1-mediated integration MD not only blocks formation, also prevents linker (NL) undocking ADP release from Mutations...
Lupus nephritis (LN) is a major determinant of morbidity and mortality in systemic lupus erythematosus (SLE). Pim-1 regulates lymphocyte proliferation activation. The role autoimmune disease remains unclear. This study was undertaken to test the hypothesis that inhibition would have therapeutic potential patients with LN.Pim-1 expression analyzed lupus-prone (NZB × NZW)F1 mice (n = 6), human peripheral blood mononuclear cells (PBMCs) from SLE 10), glomeruli LN 8). effect inhibitor AZD1208...
Rob, which serves as a paradigm of the large AraC/XylS family transcription activators, regulates diverse subsets genes involved in multidrug resistance and stress response. However, underlying mechanism how it engages bacterial RNA polymerase promoter DNA to finely respond environmental stimuli is still elusive. Here, we present two cryo-EM structures Rob-dependent activation complex (Rob-TAC) comprising Escherichia coli (RNAP), Rob-regulated Rob alternative conformations. The show that...
Transcription activation is established through extensive protein-protein and protein-DNA interactions that allow an activator to engage remodel RNA polymerase. SoxS, a global transcription activator, diversely regulates subsets of stress response genes with different promoters, but the detailed SoxS-dependent initiation mechanisms remain obscure. Here, we report cryo-EM structures three complexes (SoxS-TACI, SoxS-TACII SoxS-TACIII) comprising Escherichia coli polymerase (RNAP), SoxS protein...
Radio-immunotherapy exploits the immunostimulatory features of ionizing radiation (IR) to enhance antitumor effects and offers emerging opportunities for treating invasive tumor indications such as melanoma. However, insufficient dose deposition immunosuppressive microenvironment (TME) solid tumors limit its efficacy. Here we report a programmable sequential therapeutic strategy based on multifunctional fusogenic liposomes (Lip@AUR-ACP-aptPD-L1) overcome intrinsic radio-immunotherapeutic...
Abstract Sen1 is an essential helicase for factor-dependent transcription termination in Saccharomyces cerevisiae, whose molecular-motor mechanism has not been well addressed. Here, we use single-molecule experimentation to better understand the determinants of its action on RNA polymerase II (Pol II) complex. We quantify translocation activity single-stranded DNA (ssDNA), finding elevated rates, high levels processivity and ATP affinities. Upon deleting N- C-terminal domains, or further...
The Bidirectional Reflectance Distribution Function (BRDF) is a critical spatial distribution parameter in the quantitative research of remote sensing and has wide range applications radiometric correction, elemental inversion, surface feature estimation. As new means BRDF modeling, UAV push-broom hyperspectral imaging limited by method, multi-angle information often difficult to obtain. In addition, random variation solar illumination during low-altitude flight makes irradiance between...
Abstract Factor-dependent termination uses molecular motors to remodel transcription machineries, but the associated mechanisms, especially in eukaryotes, are poorly understood. Here we use single-molecule fluorescence assays characterize real time composition and catalytic states of Saccharomyces cerevisiae complexes remodeled by Sen1 helicase. We confirm that takes RNA transcript as its substrate translocates along it hydrolyzing multiple ATPs form an intermediate with a stalled polymerase...
This study aimed to investigate the function and signaling pathway of Toll-like receptor 4 (TLR4) in oxidized low-density lipoprotein (oxLDL)-induced up-regulated expressions LDL 1 (LOX-1), monocyte chemoattractant protein (MCP-1), vascular cell adhesion molecule (VCAM-1) human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with different oxLDL concentrations (0, 20, 40, 60, 80 μg/mL) for 24 48 h. The influence on TLR4, LOX-1, MCP-1, VCAM-1 was detected by real-time...
This study aimed to analyze the correlation between phenotype and genotype of Chinese patients with early-onset lamin A (LMNA)-related muscular dystrophy (MD). The clinical myopathological data 21 pediatric LMNA-related MD were collected analyzed. LMNA gene mutation analysis was performed by direct sequencing genomic DNA. Sublocalization wild-type mutant proteins observed immunofluorescence using cultured fibroblasts human embryonic kidney 293 (HEK 293) cell. Seven diagnosed Emery-Dreifuss...