A5008266972- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Nitrogen and Sulfur Effects on Brassica
- Catalytic Cross-Coupling Reactions
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- Studies on Chitinases and Chitosanases
- Photoreceptor and optogenetics research
- Catalytic C–H Functionalization Methods
- Metalloenzymes and iron-sulfur proteins
- Higher Education Practises and Engagement
- Higher Education Research Studies
- Machine Learning in Materials Science
- Synthetic Organic Chemistry Methods
- Asymmetric Hydrogenation and Catalysis
- Mentoring and Academic Development
- Oxidative Organic Chemistry Reactions
- Metabolism and Genetic Disorders
- Biochemical and Structural Characterization
- ATP Synthase and ATPases Research
- Metal complexes synthesis and properties
- Evolution and Genetic Dynamics
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
University of California, Berkeley
2020-2025
Lawrence Berkeley National Laboratory
2021-2024
QB3
2022
University of Utah
2014-2017
Salt Lake Regional Medical Center
2014
Aschaffenburg University of Applied Sciences
1881
Prokaryotic nanocompartments, also known as encapsulins, are a recently discovered proteinaceous organelle-like compartment in prokaryotes that compartmentalize cargo enzymes. While initial studies have begun to elucidate the structure and physiological roles of bioinformatic evidence suggests great diversity encapsulin nanocompartments remains unexplored. Here, we describe novel freshwater cyanobacterium
Significance Product distributions of chemical reactions are dictated by a myriad interactions between molecular species. Identifying which these features affects reaction selectivity is key facet for mechanistically understanding transformation. Such insight often facilitates optimization as well indicates types substrates (substrate scope) suited to the method. Unfortunately, assessment impactful frequently qualitative endeavor that would significantly benefit from quantitation. We...
A new enzymatic method is reported for constructing protein- and DNA-AuNP conjugates. The strategy relies on the initial functionalization of AuNPs with phenols, followed by activation enzyme tyrosinase. Using an oxidative coupling reaction, activated phenols are coupled to proteins bearing proline, thiol, or aniline functional groups. Activated phenol-AuNPs also conjugated a small molecule biotin commercially available thiol-DNA. Advantages this approach AuNP bioconjugation include: (1)...
Photosynthetic organisms utilize dynamic and complex networks of pigments bound within light-harvesting complexes to transfer solar energy from antenna reaction centers. Understanding the principles underlying efficiency these processes, how they may be incorporated into artificial systems, is facilitated by construction easily tunable model systems. We describe a protein-based mimic directional between using circular permutant tobacco mosaic virus coat protein (cpTMV), which self-assembles...
Instrumental resolution of Fourier transform-charge detection mass spectrometry instruments with electrostatic ion trap individual ions depends on the precision which energy is determined. Energy can be selected using optic filters or from harmonic amplitude ratios (HARs) that provide Fellgett's advantage and eliminate necessity transmission loss to improve resolution. Unlike energy-filtering method, HAR method increases charge (improved S/N) thus mass. An analysis current instrumentation...
Pd-catalyzed allylic relay Suzuki cross-coupling reactions of secondary alkyl tosylates, featuring a sterically-hindered oxidative addition and precise control β-hydride elimination, are reported. The identification linear free energy relationship between the relative rates substrate consumption electronic nature alkene suggests that requires direct involvement. A study effect chain length on reaction outcome supports chelation-controlled addition.
Short-time Fourier transforms with short segment lengths are typically used to analyze single ion charge detection mass spectrometry (CDMS) data either overcome effects of frequency shifts that may occur during the trapping period or more precisely determine time at which an changes charge, enters unstable orbit. The can lead scalloping loss unless a large number zero-fills used, making computational significant factor in real-time analysis data. Apodization specific fitting leads 9-fold...
The undergraduate transfer process has well-documented challenges, especially for those who identify with groups historically excluded from science, technology, engineering, and mathematics (STEM) programs. Because students gain later access to university networking research opportunities than first-time-in-college students, interested in pursuing postbaccalaureate degrees chemistry have a significantly shortened timeline which conduct research, crucial component graduate school...
Photosynthetic light harvesting requires efficient energy transfer within dynamic networks of light-harvesting complexes embedded phospholipid membranes. Artificial models are valuable tools for understanding the structural features underpinning absorption and chromophore arrays. Here, a method attaching protein-based model to planar, fluid supported lipid bilayer (SLB) is developed. The protein consists tobacco mosaic viral capsid proteins that gene-doubled create tandem dimer (dTMV)....
Abstract Prokaryotic nanocompartments, also known as encapsulins, are a recently discovered proteinaceous organelle in prokaryotes that compartmentalize cargo enzymes. While initial studies have begun to elucidate the structure and physiological roles of bioinformatic evidence suggests great diversity encapsulin nanocompartments remains unexplored. Here, we describe novel freshwater cyanobacterium Synechococcus elongatus PCC 7942. This nanocompartment is upregulated upon sulfate starvation...
The tobacco mosaic viral capsid protein (TMV) is a frequent target for derivatization myriad applications, including drug delivery, biosensing, and light harvesting. However, solutions of the stacked disk assembly state TMV are difficult to characterize quantitatively due their large size multiple assembled states. Charge detection mass spectrometry (CDMS) addresses need heterogeneous populations complexes in solution quickly accurately. Using CDMS, previously unobserved states TMV,...
The bis(pyridine)silver(I) permanganate promoted hydroxylation of diketopiperazines has served as a pivotal transformation in the synthesis complex epipolythiodiketopiperazine alkaloids. This late-stage C-H oxidation chemistry is strategically critical to access N-acyl iminium ion intermediates necessary for nucleophilic thiolation advanced en route potent anticancer compounds. In this study, we develop an informative mathematical model using hydantoin derivatives training set substrates by...
Despite extensive studies, many questions remain about what structural and energetic factors give rise to the remarkable energy transport efficiency of photosynthetic light-harvesting protein complexes, owing largely inability synthetically control such in these natural systems. Herein, we demonstrate transfer within a biomimetic complex consisting identical chromophores attached circular array scaffold derived from tobacco mosaic virus coat protein. We confirm capability by observing...
Abstract Mechanistic studies suggest that the oxidative addition requires direct alkene involvement.
The undergraduate transfer process has well-documented challenges, especially for those who identify with groups histori-cally excluded from science, technology, engineering, and mathematics (STEM) programs. Because students gain later access to university networking research opportunities than first-time-in-college students, inter-ested in pursuing post-baccalaureate degrees chemistry have a significantly shortened timeline which conduct re-search, crucial component graduate school...
Abstract Photosynthetic light harvesting requires efficient energy transfer within dynamic networks of complexes embedded phospholipid membranes. Artificial models are valuable tools for understanding the structural features underpinning absorption and chromophore arrays. Most artificial static or in solution phase, rather than a two-dimensional fluid environment as natural photosynthesis. We have developed method attaching protein-based model to supported lipid bilayer (SLB), which provides...